TargeTron

Genetic Engineering that is Right on Target! Introduction to TargeTron The TargeTron Gene Knockout System ( TA0100 ) is a revolutionary method for rapid and specific disruption of genes in prokaryotic organisms. Utility of the technology has been demonstrated for prokaryotic genetic engineering, systems biology and functional genomics approaches. View Technical Animation and NEW presentations on TargeTron and group II intron biology ! Features Targeted and permanent gene disruption Simple, streamlined protocol; Knockouts in 3 days or less Minimal screening to isolate mutants >90% successful targeted insertion No specific host factor requirements Pre-screen your target sequence on the TargeTron Test Site   Resources TargeTron Vectors Detailed Description and Algorithm TargeTron Test Site TargeTron Design Site User Guide (1.1 MB PDF) pACD4K-C Vector Sequence (7.7 KB TXT) Updated License Agreement (86 KB PDF) Poster (1.6 MB PDF) Frequently Asked Questions References Ordering Information Host Range TargeTron has been validated in multiple organisms; however, work to adapt the system to other organisms is ongoing and data will be provided as it becomes available. Organism Reference Agrobacterium tumefaciens Gene Targeting in Gram-Negative Bacteria by Use of a Mobile Group II Intron ("Targetron") Expressed from a Broad-Host-Range Vector Bacillus subtilis (TargeTron Vector pNL9164, Product No. T6701 ) — Clostridium acetobutylicum The ClosTron: A universal gene knock-out system for the genus Clostridium Clostridium botulinum The ClosTron: A universal gene knock-out system for the genus Clostridium Clostridium difficile The ClosTron: A universal gene knock-out system for the genus Clostridium Clostridium perfringens (TargeTron Vector pJIR750ai, Product No. T7701 ) Disruption of a toxin gene by introduction of a foreign gene into the chromosome of Clostridium perfringens using targetron-induced mutagenesis Clostridium sporogenes The ClosTron: A universal gene knock-out system for the genus Clostridium Escherichia coli (TargeTron Gene Knockout System, Product No. TA0100 ) — Lactococcus lactis (TargeTron Gene Knockout System, Product No. TA0100 ) — Proteus mirabilis Reference in press Pseudomonas aeruginosa Gene Targeting in Gram-Negative Bacteria by Use of a Mobile Group II Intron ("Targetron") Expressed from a Broad-Host-Range Vector Salmonella typhimurium (TargeTron Gene Knockout System, Product No. TA0100 ) — Serratia marcescens Unpublished to date Shigella flexneri (TargeTron Gene Knockout System, Product No. TA0100 ) — Staphylococcus aureus (TargeTron Vector pNL9164, Product No. T6701 ) — Xylella fastidiosa Unpublished to date Back to Top Related Products Below is an easy guide to materials needed for or that complement the TargeTron Gene Knockout System. TargeTron Vectors GenElute™ PCR Clean-up Kit ( NA1020 ) Quick Link™ DNA Ligation Kit ( LIG-2 ) Water, Molecular Biology Reagent ( W4502 ) GC5™ Competent Cells ( G3169 ) BL21 (DE3)-T1 R Competent Cells ( B2935 ) SOC Medium ( S1797 ) LB Broth, liquid ( L2542 ) LB Agar, EZ Mix™ Powder ( L7533 ) LB agar plates IPTG ( I6758 ) X-gal ( B9146 ) Pre-cast Agarose Gels   Related Technologies Functional Genomics siRNA MISSION® shRNA   This product and its use are the subject of one or more of U.S. Patent Nos. 5,698,421 804,418 869,634 027,895 001,608, and 6,306,596 and/or other pending U.S. and foreign patent applications controlled by InGex, LLC. TargeTron is a registered trademark of InGex, LLC.