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Functional Genomics & RNAi
TargeTron | ||
Genetic Engineering that is Right on Target!
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Introduction to TargeTron |
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Features
- Targeted and permanent gene disruption
- Simple, streamlined protocol; Knockouts in 3 days or less
- Minimal screening to isolate mutants
- >90% successful targeted insertion
- No specific host factor requirements
- Pre-screen your target sequence on the TargeTron Test Site
Resources
TargeTron Vectors
Detailed Description and Algorithm
TargeTron Test Site
TargeTron Design Site
User Guide (1.1 MB PDF)
pACD4K-C Vector Sequence (7.7 KB TXT)
Updated License Agreement (86 KB PDF)
Frequently Asked Questions
References
Ordering Information
Host Range
TargeTron has been validated in multiple organisms; however, work to adapt the system to other organisms is ongoing and data will be provided as it becomes available.
Related Products
Below is an easy guide to materials needed for or that complement the TargeTron Gene Knockout System.
- TargeTron Vectors
- GenElute™ PCR Clean-up Kit ( NA1020 )
- Quick Link™ DNA Ligation Kit ( LIG-2 )
- Water, Molecular Biology Reagent ( W4502 )
- GC5™ Competent Cells ( G3169 )
- BL21 (DE3)-T1 R Competent Cells ( B2935 )
- SOC Medium ( S1797 )
- LB Broth, liquid ( L2542 )
- LB Agar, EZ Mix™ Powder ( L7533 )
- LB agar plates
- IPTG ( I6758 )
- X-gal ( B9146 )
- Pre-cast Agarose Gels
Related Technologies
Functional Genomics
This product and its use are the subject of one or more of U.S. Patent Nos. 5,698,421, 5,804,418, 6,027,895 and 6,001,608.
| Organism | Reference |
| Agrobacterium tumefaciens | Gene Targeting in Gram-Negative Bacteria by Use of a Mobile Group II Intron ("Targetron") Expressed from a Broad-Host-Range Vector |
| Bacillus subtilis (TargeTron Vector pNL9164, Product No. T6701 ) | — |
| Clostridium acetobutylicum | The ClosTron: A universal gene knock-out system for the genus Clostridium |
| Clostridium botulinum | The ClosTron: A universal gene knock-out system for the genus Clostridium |
| Clostridium difficile | The ClosTron: A universal gene knock-out system for the genus Clostridium |
| Clostridium perfringens (TargeTron Vector pJIR750ai, Product No. T7701 ) | Disruption of a toxin gene by introduction of a foreign gene into the chromosome of Clostridium perfringens using targetron-induced mutagenesis |
| Clostridium sporogenes | The ClosTron: A universal gene knock-out system for the genus Clostridium |
| Escherichia coli (TargeTron Gene Knockout System, Product No. TA0100 ) | — |
| Lactococcus lactis (TargeTron Gene Knockout System, Product No. TA0100 ) | — |
| Proteus mirabilis | Reference in press |
| Pseudomonas aeruginosa | Gene Targeting in Gram-Negative Bacteria by Use of a Mobile Group II Intron ("Targetron") Expressed from a Broad-Host-Range Vector |
| Salmonella typhimurium (TargeTron Gene Knockout System, Product No. TA0100 ) | — |
| Serratia marcescens | Unpublished to date |
| Shigella flexneri (TargeTron Gene Knockout System, Product No. TA0100 ) | — |
| Staphylococcus aureus (TargeTron Vector pNL9164, Product No. T6701 ) | — |
| Xylella fastidiosa | Unpublished to date |