Vector pJIR750ai

TargeTron vector pJIR750ai is a circularized vector that can be used for targeted gene knockouts in gram-positive bacteria such as Clostridium perfringens type A.¹ Expression of the group II intron RNA is under the control of the β-2 toxin gene promoter, cpb2.¹ As supplied, the pJIR750ai vector is re-targeted to the α-toxin (plc) gene. α-Toxin, a phospholipase C, is an extracellular toxin produced by all C. perfringens isolates.¹ For validation in a specific C. perfringens strain, this vector can be used directly to knockout plc without any further modifications to the enclosed protocol. In order to re-target this vector to knockout other genes, the plc specific IBS-EBS fragment (350 bp) between the Hind III and BsrG I sites can be cut out and replaced with another gene specific fragment. For re-targeting protocols, refer to the TargeTron Gene Knockout System, TA0100 User Guide (1.16 MB PDF).

Technical Bulletin Vector Map Vector Sequence(11 Kb txt file) Ordering Information References Chen, Y. et. al, Appl Environ Microbiol. 71(11): 7542-7547(2005).   Related Products TargeTron Gene Knockout System

TargeTron is a registered trademark of InGex, LLC.

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TargeTron® Gene Knockout System