Biofiles 4.7

Reagents for Quantitative PCR and RT-PCR

Sigma offers a broad array of qPCR kits for all detection chemistries and all instrument platforms. Researchers can simply choose between
SYBR® Green and Probe-based detection, then select from the following formats:

  • Standard qPCR - kits for plate-based and tube-based instruments
  • Capillary tube qPCR - kits for the Roche LightCycler® 2.0 & 1.0
  • High-Throughput qPCR - kits with reference dye premixed into the ReadyMix. Compatible with most ABI instruments
  • One-Step qRT-PCR - kits that include reverse transcriptase for streamlined quantification of RNA levels

SYBR® Green Detection

Compatible Platforms: ABI instruments; Roche LightCycler® 480; Bio-Rad/MJ instruments; Stratagene instruments; Corbett Rotor- Gene 6000 and Rotor-Gene 3000; Eppendorf Mastercycler® ep realplex instruments
Name Cat. No
SYBR® Green JumpStart™ Taq ReadyMix™ S4438
SYBR® Green JumpStart™ Taq ReadyMix™ S5193
Compatible Platforms: Roche LightCycler 2.0 & 1.0
Name Cat. No.
SYBR® Green JumpStart™ Taq ReadyMix™ for Quantitative PCR, Capillary Formulation S1816
SYBR® Green JumpStart™ Taq ReadyMix™ S5193
 
High Throughput qPCR
Compatible Platforms: ABI 7300, 7700, 7900, and StepOne™
Name Cat. No.
SYBR® Green JumpStart™ Taq ReadyMix™ for High Throughput QPCR S9194
One-Step qRT-PCR
Compatible Platforms: ABI instruments; Roche LightCycler 480; Roche LightCycler 2.0 & 1.0; Bio-Rad/MJ instruments; Stratagene instruments; Corbett Rotor-Gene 6000 and Rotor-Gene 3000; Eppendorf Mastercycler ep realplex instruments
Name Usage Cat. NO
SYBR® Green Quantitative RT-PCR Kit 100 PCR reactions (50 µl) QR0100
 

Probe-based Detection

Standard qPCR
Compatible Platforms: ABI instruments; Roche LightCycler 480; Bio-Rad/MJ instruments; Stratagene instruments; Corbett Rotor- Gene 6000 and Rotor-Gene 3000; Eppendorf Mastercycler ep realplex instruments
Name Cat. No.
JumpStart™ Taq ReadyMix™ for Quantitative PCR D7440
JumpStart™ Taq ReadyMix™ with dUTP D9191
High-Throughput qPCR
Compatible Platforms: ABI 7300, 7700, 7900, and StepOne
Name Cat. No.
JumpStart™ Taq ReadyMix™ for High Throughput Quantitative PCR D6442
 
One-Step qRT-PCR
Compatible Platforms: ABI instruments; Roche LightCycler 480; Roche LightCycler 2.0 & 1.0; Bio-Rad/MJ instruments; Stratagene instruments; Corbett Rotor-Gene 6000 and Rotor-Gene 3000; Eppendorf Mastercycler ep realplex instruments
Name Usage Cat. No.
Quantitative RT-PCR ReadyMix™ 100 PCR reactions (50 µl) QR0200
 
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