Reagents for Quantitative PCR and RT-PCR

Sigma offers a broad array of qPCR kits for all detection chemistries and all instrument platforms. Researchers can simply choose between
SYBR® Green and Probe-based detection, then select from the following formats:

Standard qPCR - kits for plate-based and tube-based instruments
Capillary tube qPCR - kits for the Roche LightCycler® 2.0 & 1.0
High-Throughput qPCR - kits with reference dye premixed into the ReadyMix. Compatible with most ABI instruments
One-Step qRT-PCR - kits that include reverse transcriptase for streamlined quantification of RNA levels

SYBR® Green Detection

Standard qPCR

Compatible Platforms: ABI instruments; Roche LightCycler® 480; Bio-Rad/MJ instruments; Stratagene instruments; Corbett Rotor- Gene 6000 and Rotor-Gene 3000; Eppendorf Mastercycler® ep realplex instruments

Name	Cat. No
SYBR® Green JumpStart^™ Taq ReadyMix^™	S4438
SYBR® Green JumpStart^™ Taq ReadyMix^™	S5193

Capillary tube qPCR

Compatible Platforms: Roche LightCycler 2.0 & 1.0

Name	Cat. No.
SYBR® Green JumpStart^™ Taq ReadyMix^™ for Quantitative PCR, Capillary Formulation	S1816
SYBR® Green JumpStart^™ Taq ReadyMix^™	S5193

High Throughput qPCR

Compatible Platforms: ABI 7300, 7700, 7900, and StepOne^™

Name	Cat. No.
SYBR® Green JumpStart^™ Taq ReadyMix^™ for High Throughput QPCR	S9194

One-Step qRT-PCR

Compatible Platforms: ABI instruments; Roche LightCycler 480; Roche LightCycler 2.0 & 1.0; Bio-Rad/MJ instruments; Stratagene instruments; Corbett Rotor-Gene 6000 and Rotor-Gene 3000; Eppendorf Mastercycler ep realplex instruments

Name	Usage	Cat. NO
SYBR® Green Quantitative RT-PCR Kit	100 PCR reactions (50 µl)	QR0100

Probe-based Detection

Standard qPCR

Compatible Platforms: ABI instruments; Roche LightCycler 480; Bio-Rad/MJ instruments; Stratagene instruments; Corbett Rotor- Gene 6000 and Rotor-Gene 3000; Eppendorf Mastercycler ep realplex instruments

Name	Cat. No.
JumpStart^™ Taq ReadyMix^™ for Quantitative PCR	D7440
JumpStart^™ Taq ReadyMix^™ with dUTP	D9191

High-Throughput qPCR

Compatible Platforms: ABI 7300, 7700, 7900, and StepOne

Name	Cat. No.
JumpStart^™ Taq ReadyMix^™ for High Throughput Quantitative PCR	D6442

One-Step qRT-PCR

Name	Usage	Cat. No.
Quantitative RT-PCR ReadyMix^™	100 PCR reactions (50 µl)	QR0200

your favorite gene search

Service & Support

Ordering

Corporate

©2011 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA, SAFC, SIGMA-ALDRICH, ALDRICH, and SUPELCO are trademarks of Sigma-Aldrich Co. LLC, registered in the US and other countries. FLUKA is a trademark of Sigma-Aldrich GmbH, registered in the US and other countries. Sigma brand products are sold through Sigma-Aldrich, Inc. Purchaser must determine the suitability of the product(s) for their particular use. Additional terms and conditions may apply. Site Use Terms | Privacy