BioFiles Volume 5, Number 4 — Cryopreservation

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Table of Contents

 


New Products



CryoStor™

The CryoStor™ line of serum free cryoprotective products allow higher recovery rates than using media with 10% FBS and DMSO (Figure 1). CryoStor allows researchers to optimize, and if necessary, minimize the amount of DMSO needed (Figure 2). The CryoStor line is serum-free so concerns of viruses, BSE or other FBS/proteinbased contaminations are eliminated. In addition, CryoStor is robust and has been tested with the following cell types.

  • Liver Cells (Hepatocytes & C3A) Cardiomyocytes
  • Stem Cells Hybridomas
  • PBMC Pancreatic Islets
  • Cord Blood Skeletal Muscle Cells (SKMC)
  • T-Cells Smooth Muscle Cells (CASMC)
  • Lymphocytes PBSC
  • Coronary Artery Endothelial Cells (CAEC) Chondrocytes
  • Cancer Cells (PC3, LNCaP) CHO
  • Skeletal Myoblasts C2C12
  • Neuronal Cells Ocular (BCE, HCE)
  • Keratinocytes (A431) Engineered Tissue
  • Renal Cells (RPTEC, MDCK) Organ / Tissue
  • Fibroblasts (NHDF, 3T3) Vascular Tissue
  • Transfected Cells Hair Follicle
  • HEK Dendritic Cells

 


CryoStor is ideal for cryopreservation applications that
must be serum free and where high recovery is critical.


Figure 1. Viability of NHDF cells following exposure to cell culture media + 5% DMSO or CryoStor + 5% DMSO (CS5). Solution efficacy was investigated following a 10 minute hold at 2–8 °C , following ice-nucleation, and after LN2 storage. For each of the test conditions, samples were removed, thawed, diluted with media and plated. Relative cell viability was determined 1 day post-thaw and overall viability was compared to 37 °C non-cryopreserved control cultures.

 

Figure 2. Post-thaw viability of cells following cryopreservation with varying concentrations of DMSO in either cell culture media or CryoStor. Relative cell viability was determined 1 day post-thaw as described in the methods and overall viability was compared to 37 °C non-cryopreserved control cultures. DMSO concentration and vehicle solution impact cell viability post-thaw.

 

CryoStor cell cryopreservation media
CryoStor, a series of cell-specific, optimized preservation media, is uniquely formulated to address the molecular and biological aspects of cells during the cryopreservation process thereby directly reducing the level of Cryopreservation-Induced Delayed-Onset Cell Death and improving post-thaw cell viability and function. (store at: 2–8 °C)

CS2 – CryoStor CS2 is formulated to contain 2% dimethyl sulfoxide (DMSO). Suggested when reducing DMSO is of primary concern.
Product No. C3124-100ML

CS5 – Cryostor CS5 is formulated to contain 5% dimethyl sulfoxide (DMSO). Recommended for cryopreservation of most cell types.
Product No. C2999-100ML

CS10 – CryoStor CS10 is a uniquely formulated cryopreservation medium containing 10% dimethyl sulfoxide (DMSO). Recommended for the preservation of hepatocytes, tissue samples and other extremely sensitive cell types.
Product No. C2874-100ML



HypoThermosol®

Figure 3 and figure 4 clearly show that HypoThermosol outperforms commonly used hypothermic preservation media on Human Mesenchymal Stem Cells (hMSC) and on Human Dermal Fibroblasts. In addition to these cell lines, hypothermosol has been tested on the following cell lines, Tissues and organs:

  • Liver Cells (Hepatocytes & C3A) Cardiomyocytes
  • Stem Cells Hybridomas
  • PBMC Pancreatic Islets
  • Cord Blood Skeletal Muscle Cells (SKMC)
  • T-Cells Smooth Muscle Cells (CASMC)
  • Lymphocytes PBSC
  • Coronary Artery Endothelial Cells (CAEC) Chondrocytes
  • Cancer Cells (PC3, LNCaP) CHO
  • Skeletal Myoblasts C2C12
  • Neuronal Cells Ocular (BCE, HCE)
  • Keratinocytes (A431) Engineered tissue
  • Renal Cells (RPTEC, MDCK) Organ / Tissue
  • Fibroblasts (NHDF, 3T3) Vascular Tissue
  • Transfected Cells Hair Follicle
  • HEK Dendritic Cells


Figure 3. Cells were assayed for metabolic activity following 24 hours recovery post-preservation.

 

Figure 4. Cells were assayed for metabolic activity following 24 hours recovery post-preservation.

 

HypoThermosol-FRS Preservation Solution
HypoThermosol is an optimized hypothermic (2–8 °C) preservation media that enables improved and extended preservation of cells, tissues and organs. HypoThermosol-FRS is uniquely formulated to address the molecular-biological response of cells during the hypothermic preservation process. HypoThermosol-FRS includes key ions at concentrations that balance the intracellular state at hypothermic temperatures. Additional components include pH buffers, energy substrates, free radical scavengers, and osmotic/oncotic stabilizers. (store at 2–8 °C)

Product No. H4416-100ML

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