Cold Spring Harbor Laboratory Protocols

Sigma® has prepared product lists for commonly used protocols from Cold Spring Harbor Protocols.

The product lists correspond to protocols for:

• Antibodies
• RNAi
• Genomics
• Neuroscience
• Proteomics
• Stem Cell Biology

Antibodies

• Removal of Cross-reactive Antibodies from Antiserum: Affinity Chromatography
  For a product list of materials for this protocol, click here.
  
  
• Histological Preparation of Embryonic and Adult Zebrafish Eyes
  
  
• Nondenaturing Protein Immunoprecipitation from Mammalian Cells
  
  
• Yeast Chromatin Immunoprecipitation (ChIP) Assay
  
  
• Epitope Mapping by Competition Assay

RNAi

Buy this book, Z700401

Selected Protocols from "Manipulating the Mouse Embryo," 3rd Edition, Andras Nagy et al.

• Culturing Trophoblast Stem (TS) Cell Lines
  For a product list of materials for this protocol, click here.
  
  
• In Situ Hybridization of Whole-Mount Mouse Embryos with RNA Probes: Hybridization, Washes, and Histochemistry
  For a product list of materials for this protocol, click here.
  
  
• Production of Endoribonuclease-Prepared Short Interfering RNAs (esiRNAs) for Specific and Effective Gene Silencing in Mammalian Cells
  For a product list of materials for this protocol, click here.

• Production of dsRNA for RNAi in Avian Embryos
  For a product list of materials for this protocol, click here.
  
  
• Microinjection of dsRNA into Mouse Oocytes and Early Embryos
  For a product list of materials for this protocol, click here.
  
  
• Selection of siRNA Sequences
  For a product list of materials for this protocol, click here.
  
  
• Annealing siRNAs to Produce siRNA Duplexes
  For a product list of materials for this protocol, click here.
  
  
• Cell Culture and Preparation of Cells in 24-Well Plates
  For a product list of materials for this protocol, click here.
  
  
• Cotransfection of Luciferase Reporter Plasmids with siRNA Duplexes
  For a product list of materials for this protocol, click here.
  
  
• Transfection of siRNA Duplexes
  For a product list of materials for this protocol, click here.
  
  
• Immunofluorescence Detection of Protein Knockdown
  For a product list of materials for this protocol, click here.
  
  
• Detection of Protein Knockdown by Western Blotting
  For a product list of materials for this protocol, click here.
  
  
• Genetic Manipulation of Mammalian Cells by Microinjection
  For a product list of materials for this protocol, click here.
  
  
• Knockdown Transgenic Mice Generated by Silencing Lentiviral Vectors
  For a product list of materials for this protocol, click here.
  
  
• Lentivirus Transduction of Hematopoietic Cells
  For a product list of materials for this protocol, click here.

Genomics

• Preparation of Biodegradable Nanoparticles and Their Use in Transfection
  For a product list of materials for this protocol, click here.
  
  
• Genome Modification by Inclusion of loxP Transgene Sequences
  For a product list of materials for this protocol, click here.
  
  
• Fate-Mapping Technique: Targeted Whole-Embryo Electroporation of DNA Constructs into the Germ Layers of Mouse Embryos 7–7.5 Days Post-coitum
  For a product list of materials for this protocol, click here.
  
  
• Generation of Transgenic Xenopus laevis
  For a product list of materials for this protocol, click here.
  
  
• Genotyping by Oligonucleotide Ligation Assay (OLA)
  For a product list of materials for this protocol, click here.
  
  
• Chromatin Immunoprecipitation (ChIP) on Unfixed Chromatin from Cells and Tissues to Analyze Histone Modifications
  For a product list of materials for this protocol, click here.
  
  
• PCR-Based Analysis of Immunoprecipitated Chromatin
  For a product list of materials for this protocol, click here.

Buy this book, M8265

Selected Protocols from "Molecular Cloning: A Laboratory Manual, 3rd ed., Vols 1, 2, and 3," Joe Sambrook and David Russell.

• Purification of PCR Products in Preparation for Cloning
  For a product list of materials for this protocol, click here.
  
  
• Selection of Poly(A)⁺ RNA by Oligo(dT)-Cellulose Chromatography
  For a product list of materials for this protocol, click here.
  
  
• Isolation of BAC DNA from Large-scale Cultures
  For a product list of materials for this protocol, click here.
  
  
• Quantitative PCR
  For a product list of materials for this protocol, click here.
  
  
• The Inoue Method for Preparation and Transformation of Competent E. coli: "Ultra Competent" Cells
  For a product list of materials for this protocol, click here.
  
  
• Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation
  For a product list of materials for this protocol, click here.
  
  
• Removal of Ethidium Bromide from DNA by Extraction with Organic Solvents
  For a product list of materials for this protocol, click here.

Neuroscience

• PEI Nanoparticles for Targeted Gene Delivery
  For a product list of materials for this protocol, click here.

Buy this book, Z652024

Selected Protocols from "Transfecting Cultured Hippocampal Neurons with an Actin-GFP Plasmid," D. Spector & R. Goldman.

• Transfecting Cultured Hippocampal Neurons with an Actin-GFP Plasmid
  For a product list of materials for this protocol, click here.

Proteomics

• Protein Interactions Captured by Chemical Cross-linking: One-Step Cross-linking with Formaldehyde
  For a product list of materials for this protocol, click here.
  
  
• cAMP Assays
  For a product list of materials for this protocol, click here.
  
  
• Using Genetically Engineered Kinases to Screen for Novel Protein Kinase Substrates
  For a product list of materials for this protocol, click here.
  
  
• Purification of Expressed Proteins from Inclusion Bodies
  For a product list of materials for this protocol, click here.
  
  
• Phosphorylation of Substrates
  For a product list of materials for this protocol, click here.
  
  
• Identification of a Mutant Kinase/ATP Analog Pair
  For a product list of materials for this protocol, click here.
  
  
• Preparation of Glutathione-S-Transferase (GST) Fusion Proteins
  For a product list of materials for this protocol, click here.
  
  
• Synthesis and Probing of Membrane-bound Peptide Arrays
  For a product list of materials for this protocol, click here.
  
  
• Fine Mapping of Genomic Targets of Nuclear Proteins in Cultured Cells
  For a product list of materials for this protocol, click here.
  
  
• Screening Kinase Phosphorylation Motifs Using Peptide Libraries
  For a product list of materials for this protocol, click here.
  
  
• Digestion of Proteins and Extraction of Peptides from an Acrylamide Gel
  For a product list of materials for this protocol, click here.
  
  
• Direct Enzymatic Digestion of Protein Complexes
  For a product list of materials for this protocol, click here.
  
• Preparation of Reversed-Phase Microcolumns
  For a product list of materials for this protocol, click here.
  
  
• Characterization of Phosphopeptides Using a Combination of Immobilized Metal Ion Affinity Media and Direct Analysis by MALDI-TOF-MS
  For a product list of materials for this protocol, click here.
  
  
• Analysis of the Topology of Protein Complexes Using Cross-Linking and Mass Spectrometry
  For a product list of materials for this protocol, click here.
  
  
• Preparation and Use of an Integrated Microcapillary HPLC Column and ESI Device for Proteomic Analysis
  For a product list of materials for this protocol, click here.
  
  
• Analysis of Complex Protein Mixtures Using Multidimensional Protein Identification Technology (MuDPIT)
  For a product list of materials for this protocol, click here.
  
  
• General Method for MALDI-MS Analysis of Proteins and Peptides
  For a product list of materials for this protocol, click here.
  
  
• Isotope-coded Affinity Tagging of Proteins
  For a product list of materials for this protocol, click here.
  
  
• In Vivo Isotopic Labeling of Proteins for Quantitative Proteomics
  For a product list of materials for this protocol, click here.

Stem Cell Biology

• Culturing Trophoblast Stem (TS) Cell Lines
  For a product list of materials for this protocol, click here.
  
  
• Preparation of Biodegradable Nanoparticles and Their Use in Transfection
  For a product list of materials for this protocol, click here.
  
  
• In Situ Hybridization of Whole-Mount Mouse Embryos with RNA Probes: Hybridization, Washes, and Histochemistry
  For a product list of materials for this protocol, click here.

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