Life Science Cold Spring Harbor Laboratory Protocols

Sigma® has prepared product lists for commonly used protocols from Cold Spring Harbor Protocols.

The product lists correspond to protocols for:

• Antibodies
• RNAi
• Genomics
• Neuroscience
• Proteomics
• Stem Cell Biology

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Antibodies Removal of Cross-reactive Antibodies from Antiserum: Affinity Chromatography For a product list of materials for this protocol, click here. Histological Preparation of Embryonic and Adult Zebrafish Eyes For a product list of materials for this protocol, click here. Nondenaturing Protein Immunoprecipitation from Mammalian Cells For a product list of materials for this protocol, click here. Yeast Chromatin Immunoprecipitation (ChIP) Assay For a product list of materials for this protocol, click here. Selected Protocols from "Using Antibodies," Ed Harlow and David Lane. Epitope Mapping by Competition Assay For a product list of materials for this protocol, click here. Buy this book, A8968 Back to Top RNAi Selected Protocols from "Manipulating the Mouse Embryo," 3rd Edition, Andras Nagy et al. Culturing Trophoblast Stem (TS) Cell Lines For a product list of materials for this protocol, click here. In Situ Hybridization of Whole-Mount Mouse Embryos with RNA Probes: Hybridization, Washes, and Histochemistry For a product list of materials for this protocol, click here. Production of Endoribonuclease-Prepared Short Interfering RNAs (esiRNAs) for Specific and Effective Gene Silencing in Mammalian Cells For a product list of materials for this protocol, click here. Buy this book, Z700401 Selected Protocols from "RNAi, A Guide to Gene Silencing," Gregory J. Hannon. Mammalian RNA Interference, Thomas Tuschl (Chapter 13, pg. 280-288) Production of dsRNA for RNAi in Avian Embryos For a product list of materials for this protocol, click here. Microinjection of dsRNA into Mouse Oocytes and Early Embryos For a product list of materials for this protocol, click here. Selection of siRNA Sequences For a product list of materials for this protocol, click here. Annealing siRNAs to Produce siRNA Duplexes For a product list of materials for this protocol, click here. Cell Culture and Preparation of Cells in 24-Well Plates For a product list of materials for this protocol, click here. Cotransfection of Luciferase Reporter Plasmids with siRNA Duplexes For a product list of materials for this protocol, click here. Transfection of siRNA Duplexes For a product list of materials for this protocol, click here. Immunofluorescence Detection of Protein Knockdown For a product list of materials for this protocol, click here. Detection of Protein Knockdown by Western Blotting For a product list of materials for this protocol, click here. Buy this book, Z701130 Selected Protocols from "Gene Transfer: Delivery and Expression of DNA and RNA," by Theodore Friedmann and John Rossi. Genetic Manipulation of Mammalian Cells by Microinjection For a product list of materials for this protocol, click here. Knockdown Transgenic Mice Generated by Silencing Lentiviral Vectors For a product list of materials for this protocol, click here. Lentivirus Transduction of Hematopoietic Cells For a product list of materials for this protocol, click here. Buy this book, Z730033 Back to Top Genomics Selected Protocols from "Gene Transfer: Delivery and Expression of DNA and RNA," by Theodore Friedmann and John Rossi. Preparation of Biodegradable Nanoparticles and Their Use in Transfection For a product list of materials for this protocol, click here. Genome Modification by Inclusion of loxP Transgene Sequences For a product list of materials for this protocol, click here. Fate-Mapping Technique: Targeted Whole-Embryo Electroporation of DNA Constructs into the Germ Layers of Mouse Embryos 7–7.5 Days Post-coitum For a product list of materials for this protocol, click here. Generation of Transgenic Xenopus laevis For a product list of materials for this protocol, click here. Genotyping by Oligonucleotide Ligation Assay (OLA) For a product list of materials for this protocol, click here. Chromatin Immunoprecipitation (ChIP) on Unfixed Chromatin from Cells and Tissues to Analyze Histone Modifications For a product list of materials for this protocol, click here. PCR-Based Analysis of Immunoprecipitated Chromatin For a product list of materials for this protocol, click here. Buy this book, Z730033 Selected Protocols from "Molecular Cloning: A Laboratory Manual, 3rd ed., Vols 1, 2, and 3," Joe Sambrook and David Russell. Purification of PCR Products in Preparation for Cloning For a product list of materials for this protocol, click here. Selection of Poly(A)+ RNA by Oligo(dT)-Cellulose Chromatography For a product list of materials for this protocol, click here. Isolation of BAC DNA from Large-scale Cultures For a product list of materials for this protocol, click here. Quantitative PCR For a product list of materials for this protocol, click here. The Inoue Method for Preparation and Transformation of Competent E. coli: "Ultra Competent" Cells For a product list of materials for this protocol, click here. Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation For a product list of materials for this protocol, click here. Removal of Ethidium Bromide from DNA by Extraction with Organic Solvents For a product list of materials for this protocol, click here. Buy this book, M8265 Back to Top Neuroscience PEI Nanoparticles for Targeted Gene Delivery For a product list of materials for this protocol, click here. Selected Protocols from "Transfecting Cultured Hippocampal Neurons with an Actin-GFP Plasmid," D. Spector & R. Goldman. Transfecting Cultured Hippocampal Neurons with an Actin-GFP Plasmid For a product list of materials for this protocol, click here. Buy this book, Z652024 Back to Top Proteomics Selected Protocols from "Protein-Protein Interactions," 2nd Edition, E. Golemis, P. Adam. Protein Interactions Captured by Chemical Cross-linking: One-Step Cross-linking with Formaldehyde For a product list of materials for this protocol, click here. cAMP Assays For a product list of materials for this protocol, click here. Using Genetically Engineered Kinases to Screen for Novel Protein Kinase Substrates For a product list of materials for this protocol, click here. Purification of Expressed Proteins from Inclusion Bodies For a product list of materials for this protocol, click here. Phosphorylation of Substrates For a product list of materials for this protocol, click here. Identification of a Mutant Kinase/ATP Analog Pair For a product list of materials for this protocol, click here. Preparation of Glutathione-S-Transferase (GST) Fusion Proteins For a product list of materials for this protocol, click here. Synthesis and Probing of Membrane-bound Peptide Arrays For a product list of materials for this protocol, click here. Fine Mapping of Genomic Targets of Nuclear Proteins in Cultured Cells For a product list of materials for this protocol, click here. Screening Kinase Phosphorylation Motifs Using Peptide Libraries For a product list of materials for this protocol, click here. Digestion of Proteins and Extraction of Peptides from an Acrylamide Gel For a product list of materials for this protocol, click here. Direct Enzymatic Digestion of Protein Complexes For a product list of materials for this protocol, click here. Buy this book, Z719145 Selected Protocol from "Proteins and Proteomics: A Laboratory Manual," Richard J. Simpson. Preparation of Reversed-Phase Microcolumns For a product list of materials for this protocol, click here. Characterization of Phosphopeptides Using a Combination of Immobilized Metal Ion Affinity Media and Direct Analysis by MALDI-TOF-MS For a product list of materials for this protocol, click here. Analysis of the Topology of Protein Complexes Using Cross-Linking and Mass Spectrometry For a product list of materials for this protocol, click here. Preparation and Use of an Integrated Microcapillary HPLC Column and ESI Device for Proteomic Analysis For a product list of materials for this protocol, click here. Analysis of Complex Protein Mixtures Using Multidimensional Protein Identification Technology (MuDPIT) For a product list of materials for this protocol, click here. General Method for MALDI-MS Analysis of Proteins and Peptides For a product list of materials for this protocol, click here. Isotope-coded Affinity Tagging of Proteins For a product list of materials for this protocol, click here. In Vivo Isotopic Labeling of Proteins for Quantitative Proteomics For a product list of materials for this protocol, click here. Buy this book, Z700428 Back to Top Stem Cell Biology Culturing Trophoblast Stem (TS) Cell Lines For a product list of materials for this protocol, click here. Preparation of Biodegradable Nanoparticles and Their Use in Transfection For a product list of materials for this protocol, click here. In Situ Hybridization of Whole-Mount Mouse Embryos with RNA Probes: Hybridization, Washes, and Histochemistry For a product list of materials for this protocol, click here. Back to Top