Cold Spring Harbor Laboratory Protocols

The following Cold Spring Harbor Laboratory Press protocols are brought to you by BioSupplyNet in a partnership with Sigma-Aldrich. A list of products is available to use in each protocol.

• Removal of Cross-reactive Antibodies from Antiserum: Affinity Chromatography
  For a product list of materials for this protocol, click here.
  
  
• Histological Preparation of Embryonic and Adult Zebrafish Eyes
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• Nondenaturing Protein Immunoprecipitation from Mammalian Cells
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• Yeast Chromatin Immunoprecipitation (ChIP) Assay
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• Epitope Mapping by Competition Assay
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• Culturing Trophoblast Stem (TS) Cell Lines
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• In Situ Hybridization of Whole-Mount Mouse Embryos with RNA Probes: Hybridization, Washes, and Histochemistry
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• Production of Endoribonuclease-Prepared Short Interfering RNAs (esiRNAs) for Specific and Effective Gene Silencing in Mammalian Cells
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• Production of dsRNA for RNAi in Avian Embryos
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• Microinjection of dsRNA into Mouse Oocytes and Early Embryos
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• Selection of siRNA Sequences
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• Annealing siRNAs to Produce siRNA Duplexes
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• Cell Culture and Preparation of Cells in 24-Well Plates
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• Cotransfection of Luciferase Reporter Plasmids with siRNA Duplexes
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• Transfection of siRNA Duplexes
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• Immunofluorescence Detection of Protein Knockdown
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• Detection of Protein Knockdown by Western Blotting
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• Genetic Manipulation of Mammalian Cells by Microinjection
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• Knockdown Transgenic Mice Generated by Silencing Lentiviral Vectors
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• Lentivirus Transduction of Hematopoietic Cells
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• Preparation of Biodegradable Nanoparticles and Their Use in Transfection
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• Genome Modification by Inclusion of loxP Transgene Sequences
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• Fate-Mapping Technique: Targeted Whole-Embryo Electroporation of
  DNA Constructs into the Germ Layers of Mouse Embryos 7–7.5 Days
  Post-coitum
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• Generation of Transgenic Xenopus laevis
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• Genotyping by Oligonucleotide Ligation Assay (OLA)
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• Chromatin Immunoprecipitation (ChIP) on Unfixed Chromatin from Cells
  and Tissues to Analyze Histone Modifications
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• PCR-Based Analysis of Immunoprecipitated Chromatin
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• Purification of PCR Products in Preparation for Cloning
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• Selection of Poly(A)⁺ RNA by Oligo(dT)-Cellulose Chromatography
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• Isolation of BAC DNA from Large-scale Cultures
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• Quantitative PCR
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• The Inoue Method for Preparation and Transformation of Competent E. coli: "Ultra Competent" Cells
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• Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation
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• Removal of Ethidium Bromide from DNA by Extraction with Organic Solvents
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• PEI Nanoparticles for Targeted Gene Delivery
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• Transfecting Cultured Hippocampal Neurons with an Actin-GFP Plasmid
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• Protein Interactions Captured by Chemical Cross-linking: One-Step
  Cross-linking with Formaldehyde
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• cAMP Assays
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• Using Genetically Engineered Kinases to Screen for Novel Protein
  Kinase Substrates
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• Purification of Expressed Proteins from Inclusion Bodies
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• Phosphorylation of Substrates
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• Identification of a Mutant Kinase/ATP Analog Pair
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• Preparation of Glutathione-S-Transferase (GST) Fusion Proteins
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• Synthesis and Probing of Membrane-bound Peptide Arrays
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• Fine Mapping of Genomic Targets of Nuclear Proteins in Cultured Cells
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• Screening Kinase Phosphorylation Motifs Using Peptide Libraries
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• Digestion of Proteins and Extraction of Peptides from an Acrylamide Gel
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• Direct Enzymatic Digestion of Protein Complexes
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• Preparation of Reversed-Phase Microcolumns
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• Characterization of Phosphopeptides Using a Combination of Immobilized Metal Ion Affinity Media and Direct Analysis by MALDI-TOF-MS
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• Analysis of the Topology of Protein Complexes Using Cross-Linking and Mass Spectrometry
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• Preparation and Use of an Integrated Microcapillary HPLC Column and ESI Device for Proteomic Analysis
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• Analysis of Complex Protein Mixtures Using Multidimensional Protein Identification Technology (MuDPIT)
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• General Method for MALDI-MS Analysis of Proteins and Peptides
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• Isotope-coded Affinity Tagging of Proteins
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• In Vivo Isotopic Labeling of Proteins for Quantitative Proteomics
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• Culturing Trophoblast Stem (TS) Cell Lines
  For a product list of materials for this protocol, click here.
  
  
• Preparation of Biodegradable Nanoparticles and Their Use in Transfection
  For a product list of materials for this protocol, click here.
  
  
• In Situ Hybridization of Whole-Mount Mouse Embryos with RNA Probes: Hybridization, Washes, and Histochemistry
  For a product list of materials for this protocol, click here.