Synthetic and Viral-based Systems for Gene Silencing Workshop

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	Overview Lecture and Lab Topics	Previous Guest Lecturers Feedback from Attendees

Overview

RNAi has emerged as an important mainstream tool for both basic and applied research, and it has revolutionized the novel discipline known as functional genomics.  This three-day workshop will provide hands-on technical training for packaging and transduction of lentiviral shRNAs, experimental design, and troubleshooting.  Featured lectures will provide in depth discussion on the fundamentals of RNA interference as well as advanced screening solutions utilizing RNAi for drug discovery and target validation.  After mastering lentiviral technology, participants will have all of the tools needed to silence genes in standard tumor cell lines, primary cells and for in vivo applications.  Registrants should be familiar with basic mammalian cell culture techniques.

Lecture and Lab Topics

Synthetic and Viral-based Systems for Gene Silencing Lectures

• Introduction to RNAi Technology
• siRNA Design and Delivery
• Monitoring siRNA Off-target Effects
• Introduction to shRNA and Lentiviral Delivery Systems
• Viral Packaging
• Lentiviral Transduction Process and Detection of Knockdown
• Monitoring shRNA Off-target Effects
• Culturing Primary Cells
• Advanced Delivery Methods for Difficult Cell Types
• Screening Strategies
• Downstream Detection Assays and Deconvolution Strategies
• Screening Applications
• In Vivo Application of RNAi

Synthetic and Viral-based Systems for Gene Silencing Labs

• Lentiviral packaging
• Lentiviral transduction
• Lentiviral Magnetic Transduction

Previous Guest Lecturers

Ross Dickins, Ph.D., Nossal Fellow at Walter and Eliza Hall Institute of Medical Research, Australia
Ernesto Guccione, Ph.D., Assistant Professor at Institute of Molecular and Cell Biology, Singapore
Daniel G. Anderson, Ph.D., David H. Koch Institute for Integrative Cancer Research, MIT
Rick Cohen, Ph.D., Director of hESC Core Facility at Rutgers
Aubrey Thompson, Ph.D., Professor of Biochemistry and Molecular Biology at Mayo Clinic
Richard Hotchkiss, M.D., Professor of Anesthesiology, Medicine and Surgery at Washington University
Michael McManus, Ph.D., Assistant Professor at UCSF Diabetes Center
Sheila Stewart, Ph.D., Assistant Professor Cell Biology and Physiology at Washington University
Manika Pal Bhadra, Ph.D., Scientist El, Institute of Chemical Technology, Hyderabad, India

Feedback from Attendees

"Absolutely awesome. Everyone at Sigma is very receptive and ready to assist with all needs. I would highly recommend this workshop to anyone in the field. I am very glad that I had this opportunity." — Laia Gómez Baldó, Institute Català d'Oncologia, Spain

"An exquisitely crafted workshop with educational goals and highly recommended." — Hwei Ling Tan, National Cancer Center, Singapore

"I just wanted to thank you for the hospitality you have shown us during our stay in St. Louis. It was a very pleasant experience and it felt very good to know that there are people that not only care about the science but also about fellow human beings. The workshop was very nicely arranged, if there were any hiccups, I did not see any. The crew was also very helpful and friendly, that counts a lot for me." — Phiyani Lebea, at CSIR Biosciences, South Africa

BioU grad, Beverly Chilton talks in depth about her experiences with our workshops.

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