Customer Education and Training

Biology and Analytical Chemistry Webinar Guide

 
Biology Webinar Guide Quality Systems and Regulatory Compliance Drug Discovery Webinar
Guide
Analytical Webinar
Guide
Materials Science Webinar Guide
 
Biology Webinar Guide
Title Topics Covered Length
Proteomics
Single Step Lysis and Purification cell lysis, protein purification, protein extraction 20 minutes
Winning Westerns western blot, protein, troubleshooting, immunoblotting, electrophoresis 60 minutes
Human Protein Atlas Webinar proteome, antibody, biomarker 36 minutes
qPCR
Primer and Probe Design
primer, probe, assay design
7 minutes
An Introduction to qPCR Concepts gene expression, mRNA, assay design 10 minutes
Selecting a qPCR Basic Detection Chemistry assay design, molecular beacons, scorpion probes, locked nucleic acid probes 13 minutes
Choosing a Fluorophore/Quencher Combination assay design, probe design 12 minutes
Chemistries for More Challenging qPCR Assays assay design, optimization, probe design 15 minutes
MIQE Concepts assay standardization, assay reproducibility 3 minutes
Reference Gene Validation normalization, reference gene, NormFinder, GeNorm, GenEX 13 minutes
Data Analysis Guidelines absolute quantification, relative quantification, statistical analysis 11 minutes
MIQE: Assay Design Considerations MIQE, assay design, sample quality, optimization 18 minutes
MIQE: Sample Derived Inhibitors sample quality, inhibitors, assay design, SPUD assay 13 minutes
MIQE: RNA Quality Considerations RNA quality, assay design, integrity assay 16 minutes
MIQE: RNA Quantity and RT Considerations RNA quantification, reverse transcription 17 minutes
PCR Webinar Series: Basic Principles of PCR, qPCR and ddPCR PCR, qPCR, ddPCR, choice of detection method 53 minutes
PCR Webinar Series: qPCR Assay Design evaluation of pre-designed assays, assay design, amplicon and transcript selection, primers, probe design, reverse transcription protocols 56 minutes
PCR Webinar Series: Importance of Sample Quality in qPCR Analysis sample quality 51 minutes
PCR Webinar Series: Improving Assay Quality by Optimization and Validation assay optimization, assay validation 50 minutes
PCR Webinar Series: Data Analysis qPCR data analysis, absolute quantification, relative quantification, expression profiling 61 minutes
PCR Webinar Series: Troubleshooting assay troubleshooting 61 minutes
CRISPR    
Methods for Validating a Gene Editing Approach to Treating Brain Disease with Zinc Fingers, TALEs, and CRISPR/Cas9 CRISPR, Cas9, TALEs, ZFN, Genome Editing, Neuroscience, quantitative Western blot 60 minutes *broadcast
November 9, 2016
Register now*
Designing the Next Phase in Genome Editing: Advanced CRISPR Applications CRISPR, Cas9, gRNA, Genome Editing 43 minutes
Sigma Pooled CRISPR Screening for Functional Genomics CRISPR, pooled screening, cell culture, FACS, genome editing 50 minutes
Sigma CRISPR Paired Nickases for Genome Editing CRISPR/Cas9, Specificity, Paired Nickase 29 minutes
Practical Utilization of CRISPR in the Laboratory Workflow CRISPR, Cas9, Genome Editing, Cell Engineering 33 minutes
Sigma Pooled CRISPR Screening for Functional Genomics CRISPR, pooled screening, cell culture, FACS, genome editing 49 minutes
Genome Editing with CRISPR Systems CRISPR/cas9, genome editing 61 minutes
SygRNA™-Synthetic Two Part CRISPR RNA System Cas9, RNA, Genome Editing, sgRNA sequencing 58 minutes
Sanger Arrayed CRISPR Screening in Partnership with Evotec Evotec Partnership, Lentiviral CRISPR KO libraries, CRISPR pooled 50 minutes
Cell Culture    
3D Nephrotoxicity applications & Nortis' microfluidic organ-on-chip technology

3D Nephrotoxicity applications using an in vitro kidney proximal tubule cell based model on a microphysiological system.

Nortis’s microfluidic organ-on-chip technology

120 minutes
Advanced Cell Culture Technology for Generation of In Vivo-like Tissue Models
2D vs 3D cell culture debate, Review of alternative approaches and the development of new technologies, Challenges facing 3D culture methods, in terms of technologies available and methods used, Showcase applications where 3D technology makes a difference, Future perspective for 3D cell culture technology and further development 60 minutes
New 3D-Printable Hydrogel-Based and Particle-Based Biomaterial Ink Platforms Hydrogel bioprinting, 3D-Painting, organ engineering 75 minutes
3D Cell Culture Innovations hanging drop, encapsulation 57 minutes
Cell Design Studio   75 minutes
Modifying Cell Lines for Target Discovery and Validation cell line engineering 27 minutes
Duolink

Amplified Detection of Proteins and their Interactions using the Duolink® Proximity Ligation Assay

Proximity ligation assay, Duolink, immunofluorescence, microscopy, immunohistochemistry 60 minutes

Introduction to Duolink and PLA Technology

Duolink, PLA, Protein Interaction

45 minutes

Integrating the Proximity Ligation Assay (PLA) into your Research Program

Antibody, PLA, Validation

48 minutes

Molecular Annotation of EGFR Signaling-Associated Complexes in Human Cancer

EGFR, Cancer, Diagnostics

52 minutes

Visualizing DNA Methylation (5mC) on a Specific Genomic Locus (SEPTIN9) in Individual Human Cancer Cells usingIn-situ Hybridization and Proximity Ligation Assays

Duolink, Methylation, SEPTIN9

36 minutes

Duolink in Biomedical Science: A Technique for Unraveling the Molecular Mechanisms and Uncovering the Hidden Ultrastructural Features of Gap Junctions

PLA, Gap Junctions, Subcellular Localization

51 minutes

Detection, quantification and visualisation of protein interactions for hit profiling by high content screening

PLA, Duolink, Protein Interaction

59 minutes

Genomics    
High Protein Yields in Shaken Cultures by EnPresso® Growth System Bacteria, yeast, growth system, high-cell-density, scale-up, cost savings, IPTG autoinduction 62 minutes
Gene Synthesis as an Alternative to Molecular Cloning Gene synthesis, synthetic DNA, cost effective, protein engg, bio-based circuit, pathway design 30 minutes
RNAi

The Future of RNAi Screening: Complementary Technologies and Advancements using CRISPR and LentiORFs

Screening, LentiORF, Rescue

50 minutes

Quality Systems and Regulatory Compliance
Title Topics Covered Length
Regulatory Compliance
Unannounced Audits: Are You Ready?
European Commission, Medical Manufacturing, Medical Devices
34 minutes
The Analysis Workflow of the New EPA Method 325 for Fenceline Monitorin Industrial – Petroleum, Oil & Gas Refineries, D & T, Environmental Laboratories - Air 51 minutes
Drug Discovery Webinar Guide
Title Topics Covered Length
Late Stage Functionalization of Drug-Like Molecules
drug discovery, C-H functionalization, structure activity relationships
61 minutes
High-capacity drug-delivery systems for cancer therapy based on poly(2-oxazoline)s POx, polymer therapeutics, drug delivery, paclitaxel, hydrophobic, drug loading 71 minutes
Small-Molecule Probes and Inhibitors
Navigating the Promise and Perils of Chemical Probes Inhibitor, Chemical Probes, Protein, Signal Transduction 47 minutes
Sulfonyl Fluorides in Chemical Biology and Drug Discovery Webinar Chemical biology, Sulfonyl fluorides, Chemical probes, Drug discovery, Target identification 60 minutes
Analytical Webinar Guide
Title Topics Covered Length
General HPLC (High Performance Liquid Chromatography)
HPLC Column Fundamentals
separation, resolution
28 minutes
HPLC Instrument Bandwidth and Optimizing Systems for High-Performance sample dispersion, flow rate, bandwidth 14 minutes
Using LC/MS to Meet the Challenges of a High Through-put Clinical Diagnostic Lab LC/MS, applications, clinical diagnostics 41 minutes
The Use of Alternate Selectivity in Reversed-Phase HPLC RP phases, separation chemistry 43 minutes
A Practical 2D-LC System Developed for Complex Pharmaceuticals LC x LC, construction, application 31 minutes
Chromogenic Media - Principle and Range of Media applications, methods 8 minutes
Kromasil Eternity HPLC Columns - Designed for Long Life pH ranges, methods, applications 17 minutes
Applying Best-in-class HPLC Column Technologies to the Analysis of Proteins and Biotherpeutics protein HPLC, peptide HPLC, biotherapeutics 60 minutes
Applying Best in Class Size Exclusion and Hydrophilic Interaction Chromatography Technologies to the Analysis of Glycoproteins SEC-MS, HILIC-MS, Glycan Analysis 57 minutes
HILIC (Hydrophilic Interaction Liquic Chromatography)
Using Silica HPLC Colums in HILIC Mode: A Practical Guide
silica, HILIC, applications, methods
44 minutes
Retention Mechanisms in HILIC: Robust Method Development HILIC, selectivity, method development 55 minutes
Fused-Core
HPLC Method Development and Transfer Utilizing Fused-Core Particle Technologies
fused-core, column efficiency
31 minutes
Ultra-Fast LC/MS/MS in Bioanalysis Using Fused-Core Columns high throughput, methods, applications 39 minutes
HPLC of Peptides: Speed and Resolution with Fused-Core Columns fused-core, capacity, efficiency 24 minutes
Ultra-High Performance using Fused-Core HPLC Columns HPLC, LC/MS, sensitivity 28 minutes
Ascentis Columns
Introducing Ascentis Express 5 um HPLC Columns with Fused-Core Technology
Ascentis Express, HPLC, pressure, speed, resolution
52 minutes
Introduction to Ascentis HPLC Columns Ascentis column, small molecule separation 23 minutes
Introducing Ascentis Express F5 with Applications and Theory F5, methods, applications 54 minutes
Introduction to Ascentis Express HPLC Columns fused-core, speed, methods, UHPLC 10 minutes
Introducing Ascentis Express ES-Cyano HPLC Columns ES-Cyano, retention, selectivity 37 minutes
Diagnostic Applications
Lung Cancer Lung, Cancer, Diagnostic, IHC, immunohistochemistry 66 minutes
Colorectal Cancer and Immunohistochemistry Colorectal cancer, Diagnosed cancer, The National Cancer Institute, Immunohistochemistry, Proteins 58 minutes
Multiplex Universal Peptide Quant Approach Using Microflow LC/MS/MS for Non-Human Preclinical PK Study of Therapeutic Monoclonal Antibodies Silumab, Therapeutics, PK Studies 52 minutes
Rapid LC/MS/MS-based Methods for Confirmatory Analysis of Opiates and Benzodiazepines
LC/MS, applications, forensics 26 minutes
Removal of Phospholipids and Proteins from Plasma Using HybridSPE plasma, sensitivity, column life 5 minutes
Breast Cancer and Immunohistochemistry Breast Cancer Awareness, detection, Immunohistochemistry, e-cadherin, p120 catenin, mammaglobin, and GATA3 59 minutes
Skin Cancer and Immunohistochemistry Skin Cancer, Diagnosed cancer, Immunohistochemical, Skin tissue pathology, Survival rates of skin cancer 62 minutes
Pediatric Cancer and IHC Childhood cancer, pediatric cancer 60 minutes
SPME (Solid Phase MicroExtraction)
Miniaturization of Sample Handling in Bioanalysis by SPME
SPME, high throughput, 96 well 49 minutes
Introduction to SPME (Solid Phase MicroExtraction) SPME, applications 69 minutes
Expanding the Capabilities of GC and MS with ionic Liquids complex mixtures, water quantitation, food/beverage analysis 41 minutes
Miscellaneous
Introduction to Karl Fischer Titration
product development, applications, water content determination
62 minutes
Introduction to Air Monitoring passive, active, methods, equipment 60 minutes
Analysis of Contaminants & Competition: Handling R&D Roadblocks method development, case studies 29 minutes
Evaluations of a New ionic Liquid Staionary Phase with PEG Selectivity GC, thermal stability 48 minutes
Ionic Liquid GC Columns for the Quantification of Trans Fatty Acids in Fats and Oils capillary column, complex lipid separation 31 minutes
The Extraction of Glycols from Water Using Supelco ENVI-Carb Plus glycol removal, methods 8 minutes
Certified Testing Materials & Proficiency Testing in the 17025 Laboratory reference material, testing, proficiency 46 minutes
Enzyme Explorer Navigation and Application web-based tool, pathway, library, substrate, inhibitor, IVD, diagnostics, genomics, drug discovery  33 minutes
H&E and PAS Troubleshooting and Technical Advice H&E, Staining in histology, Diagnostics, Oncology 62 minutes
Flow Cytometry
Guava Flow Cytometry and Duolink PLA Webinar Proximity ligation assay, Duolink, immunofluorescence, Guava, flow cytometry 40 minutes
Quantitative Image Analysis and Emerging Applications on the ImageStream ImageStream, Flow cytometer, Fluorescence, Nuclear localization, Macrophages 55 minutes
Multispectral Imaging Flow Cytometry: Illuminating T cell APC Interaction T cells, antigen presenting cells, Immunological synapses, ImageStream, Flow Cytometry 56 minutes
New Guava and Muse Algae Kits for Flow Cytometry Technology description, novel methods for algal characterization, emerging applications 39 minutes
Materials Science Webinar Guide
Title Topics Covered Length
Introduction to Gold Nanoparticles - Properties and Applications in Life Science optical and physical properties of gold nanoparticles, surface modification strategies, biomedical applications 44 minutes
Graphene-Based Nanomaterials for Versatile Biosensors electronic and chemical properties, reduced grapheme oxide (rGO), crumpled grapheme (CG), and vertically-oriented grapheme (VG) 61 minutes
Graphene and new monoatomic materials: using 2-dimensional nanosheets in a 3-dimensional world Graphene, supramolecular chemistry, 2D to 3D, 2D meta-materials, conductive, insulating, nanosheets 73 minutes

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