Customer Education

Sigma® Life Sciences Presents a Technical Webinar – High Protein Yields in Shaken Cultures by EnPresso® Growth System

Webinar Title: High Protein Yields in Shaken Cultures by EnPresso® Growth System



 Abstract

EnPresso, a proprietary growth system from BioSilta Ltd, applies slow enzymatic glucose release to control the growth and protein synthesis rate in bacteria and yeasts. This high-cell-density growth system can be used in any shaken cultivation device at various scales. In this webinar, Dr. Kaisa Ukkonen and Dr. Antti Vasala will review the basic requirements for successful high-cell-density cultivations and discuss common reasons for failures in E. coli-based protein production, the focus being on the cultivation conditions. In particular, prerequisites for successful scale-up will be presented. The webinar also features examples on the use of EnPresso demonstrating the time and cost savings enabled by this powerful growth system, and presents an interesting alternative protein expression protocol: IPTG-based autoinduction.
 

 What Will You Learn?

  • Review of the obstacles in protein production (reasons for poor productivity)
  • Insight into the enzymatic glucose release system
  • How to maintain or improve the productivity at scale-up
  • Recent applications of EnPresso® B Growth Systems

 Who Should Attend?

Researchers who need to express functional protein in bacterial shaker culture systems.

 

Speaker Bio
Dr. Kaisa Ukkonen
Head of Protein Expression Technologies Division
BioSilta
Kaisa has worked as an R&D Scientist at BioSilta since 2008. In 2012 she was appointed as the Head of BioSilta’s Protein Expression Technologies Division. She has authored several research papers focusing on shaken microbial cultivations. Her doctoral thesis (2014) deals with the EnBase technology and oxygen transfer in shaken cultures. She has been involved in the development of all EnPresso products.

 

Speaker Bio
Dr. Antti Vasala
Research and Development Director
BioSilta
Antti has more than 20 years of experience in the development of microbial cell culturing methods, molecular biology and recombinant protein production. Before joining BioSilta, he held various positions in the Bioprocess Engineering Laboratory, University of Oulu and was involved in the original development of EnBase technology while working in the BioProcess Engineering Laboratory, University of Oulu under the leadership of Professor Peter Neubauer. He has been an author in all the patents either licensed to or submitted by BioSilta.

 


For more information, email technicalworkshops@sial.com.