What’s in your Flask? Global Answers from Authentication Testing

 What Does it Cover?

Cell culture underpins research throughout the life sciences. Continuous cell lines have been used as culture models for many cell types, and assembled into panels for drug discovery and development. However, problems like cross-contamination are quite common and can render some cell lines unsuitable as research models. Authentic cells are typically overgrown by the contaminant over several passages to give a misidentified or false cell line.

Misidentified cell lines can be detected through authentication testing. Short tandem repeat (STR) profiling was subsequently introduced as an international reference method, and a consensus standard for human cell line authentication published by the American National Standards Institute (ANSI). International agreement has allowed all laboratories to compare their cell line stocks, resulting in a new wave of misidentified cell lines being discovered.

Funding bodies and journals are moving to set requirements for reporting of preclinical research. A good understanding of cell line provenance is also essential.

 What Will You Learn?

  • Learn about a global perspective on the problem of cell line misidentification and its implications
  • Learn about the latest technology in authentication testing and its impact on reproducibility
  • Learn about the requirements for publication and for reporting preclinical research

 

Speaker Bio
Amanda Capes-Davis
Chair Person
International Cell Line Authentication Committee (ICLAC)
Amanda Capes-Davis is a medical graduate from the University of Sydney. Amanda was the Founding Manager of CellBank Australia at the Children’s Medical Research Institute (CMRI). She was a member of the ATCC Standard Development Organization ASN-0002 workgroup, which was responsible for drafting a written ANSI standard on authentication of human cell lines. Amanda currently chairs the International Cell Line Authentication Committee (ICLAC), a voluntary scientific committee that aims to make cell line misidentification more visible and promote awareness and authentication testing as effective ways to combat the problem.