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Enzyme Explorer

Luciferase

Purified Enzymes  
Product Name Source Product No.
Recombinant Firefly    
Luciferase, recombinant, expressed in E. coli, lyophilized powder, ≥2×1010 units/mg protein Photinus pyralis
SRE0045
Luciferase, recombinant, expressed in E. coli, buffered aqueous solution, ≥2×1010 units/mg protein Photinus pyralis
L9420
Native Firefly    
Luciferase, Lyophilized powder, 10-25 × 106 light units/mg protein Photinus pyralis
L9506
Bacterial    
Luciferase, Lyophilized powder Vibrio fischeri
L8507
Kits & Reagents for ATP Determination  See data below back to top
 
Product Name Source Product No.
Adenosine 5′-triphosphate (ATP) assay kit Photinus pyralis
FL-AA
Adenosine 5′-triphosphate (ATP) assay mix, Lyophilized powder Photinus pyralis
FL-AAM
Adenosine 5′-triphosphate (ATP) assay mix dilution buffer, Lyophilized powder  
FL-AAB
Adenosine 5′-triphosphate (ATP) bioluminescent somatic cell assay kit Photinus pyralis
FL-ASC
Adenosine 5′-triphosphate (ATP) standard disodium salt trihydrate, Lyophilized powder  
FL-AAS
Somatic cell ATP releasing reagent  
FL-SAR
Firefly Extracts for Bioluminescence  
 
Product Name Source Product No.
Firefly Lantern Extract, Powder Photinus pyralis
F3641
Firefly Lantern Extract Photinus pyralis
FLE-250
Firefly diluent, Lyophilized powder  
F3766
Substrates  
 
Product Name Source Product No.
D-Luciferin Synthetic
L9504
D-Luciferin Synthetic, SigmaUltra, minimum 99% (HPLC)
L6152
D-Luciferin sodium salt, Lyophilized powder Synthetic
L6882
Antibodies   back to top
 
Product Name Specificity Product No.
Monoclonal Anti-Luciferase from mouse, approx. 2 mg/ml, Buffered aqueous solution, Purified immunoglobulin, Clone LUC-1 Photinus pyralis
L2164
Anti-Luciferase from rabbit, IgG fraction of antiserum, Buffered aqueous solution Photinus pyralis
L0159
Anti-Luciferase antibody produced in chicken, affinity isolated antibody, buffered aqueous solution   GW20091F
Anti-Luciferase Antibody, Firefly   AB3256-100UL
Anti-Luciferase Antibody, clone21   05-603

 

Sigma Luciferase ATP Determination kits are effective for determining the ATP concentrations in samples ranging from 2x10-12 to 2x10-9 moles/liter.

 

ATP is hydrolyzed and light is emitted when firefly luciferase catalyzes the oxidation of D-luciferin. Reaction (1) is reversible and the equilibrium lies far to the right. Reaction (2) is essentially irreversible.1,2 When ATP is the limiting reagent, the light emitted is proportional to the ATP present.

Luciferase ATP Determination Kits

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