Enzyme Explorer

CPS-1


Chemiluminescent Peroxidase Substrates for Western Blotting and ELISA

 Higher Sensitivity
 Lower Background
 Better Linearity

Sigma's CPS Chemiluminescent Peroxidase Substrate Systems can be used for the highly sensitive detection of peroxidase labeled material in a variety of Western blotting and ELISA applications. These substrate systems are based on an enhanced luminol product with stabilized peroxide buffer solutions that provide maximum sensitivity with minimal background interference and superior linearity.

Compare the results, compare the price, and make the obvious choice.

 

CPS-1 Chemiluminescent Peroxidase Substrate

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CPS Formulations for Western Blotting

CPS-1 Chemiluminescent Peroxidase Substrate-1
CPS-3 Chemiluminescent Peroxidase Substrate-3



CPS-1 Chemiluminescent Peroxidase Substrate-1

CPS-1 is formulated for maximum sensitivity to target extremely low abundance proteins. The component ratios of Sigma CPS-1 can be adjusted for optimal signal duration, sensitivity and background.

CPS-1 versus the Competition
Chemiluminescent detection of Bacterial Alkaline Phosphatase in an E. coli lysate using CPS-1 and the three leading competitor's products:
Use 0.125 ml per cm2 of membrane

CPS-1 Chemiluminescent Peroxidase Substrate

Ordering Information

Product Code Product Name Quantity Technical Manual
CPS1-60 Chemiluminescent Peroxidase Substrate-1 60 ml
CPS1-120 Chemiluminescent Peroxidase Substrate-1 120 ml
CPS1-300 Chemiluminescent Peroxidase Substrate-1 300 ml

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CPS-3 Chemiluminescent Peroxidase Substrate-3

Sigma's new CPS-3 formulation is an economic alternative for high-throughput Western applications that still outperforms the most commonly used competitor's products. This two-component system provides low nanogram detection with superior signal-to-noise ratio and less background. In addition, the components are stable after mixing, adding to flexibility of usage.

Features and Benefits

  • CPS-3 delivers low nanogram protein detection with superior signal-to-noise ratio.
  • Two-component system is stable for at least 6 weeks after mixing, no need to prepare fresh working solutions daily.

 

CPS-3 versus the Competition
Chemiluminescent detection of a FLAG®-bacterial alkaline phosphatase (BAP) fusion protein in a COS-7 cell lysate using CPS-3 and the three leading competitor's products:

CPS-3 Chemiluminescent Peroxidase Substrate

Ordering Information

Product Code Product Name Quantity Technical Manual
CPS3-50 Chemiluminescent Peroxidase Substrate-3 50 ml
CPS3-100 Chemiluminescent Peroxidase Substrate-3 100 ml
CPS3-500 Chemiluminescent Peroxidase Substrate-3 500 ml

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Kits for Western Blotting

For Mouse Monoclonal IgG Antibodies
For Rabbit Polyclonal Antibodies
For FLAG® Epitope-tagged Fusion Proteins



For Mouse Monoclonal IgG Antibodies

ProteoQwest™ Chemiluminescent Western Blotting Kit, PQ0201

Designed for high sensitivity chemiluminescent detection of as little as 0.03 ng of protein. It uses the popular Horseradish Peroxidase (HRP) enzyme and includes using CPS-1 Chemiluminescent Reagent and antibodies used in a Western blot specific for mouse monoclonal IgG. The chemiluminescent reaction occurs directly on the protein-immobilized membrane. This kit is optimized for use with either film or chemiluminescent imagers. All components of the ProteoQwest Chemiluminescent Western Blotting Kit have been extensively tested and optimized to ensure superb results. This kit is designed for 12 mini-gel (10 x 10 cm) blots. Kit includes Chemichrome Western Control, Western Blocker Solution, Tris Buffered with Tween 20™, Rabbit Anti-Mouse IgG Peroxidase conjugated antibody, Chemiluminescent Reaction Buffer and Chemiluminescent Reagent.

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For Rabbit Polyclonal Antibodies

ProteoQwest™ Chemiluminescent Western Blotting Kit, PQ0221

Designed for high sensitivity, low background chemiluminescent detection of as little as 0.06 ng of protein using CPS-1 Chemiluminescent Reagent. All of the components of this ProteoQwest kit have been extensively tested and optimized. This kit is designed for 12 mini-gel sized (10 x 10 cm) blots. Kit includes Chemichrome Ultimate MW Marker, Tris Buffered Saline, pH 8.0 with 3% milk, Tris Buffered Saline with TWEEN® 20, Goat Anti-rabbit IgG Peroxidase conjugated antibody, Chemiluminescent Reagent and Chemiluminescent Reaction Buffer.

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For FLAG® Epitope-tagged Fusion Proteins

ProteoQwest™ FLAG® Chemiluminescent Western Blotting Kit, PQ0400

Designed for high sensitivity chemiluminescent detection of as little as 0.03 ng of protein. It uses the popular Horseradish Peroxidase (HRP) enzyme and includes using CPS-1 Chemiluminescent Reagent and antibodies used in a Western blot specific for mouse monoclonal IgG. The chemiluminescent reaction occurs directly on the protein-immobilized membrane. This kit is optimized for use with either film or chemiluminescent imagers. All components of the ProteoQwest Chemiluminescent Western Blotting Kit have been extensively tested and optimized to ensure superb results. This kit is designed for 12 mini-gel (10 x 10 cm) blots. Kit includes Chemichrome Western Control, Western Blocker Solution, Tris Buffered with Tween 20™, Rabbit Anti-Mouse IgG Peroxidase conjugated antibody, Chemiluminescent Reaction Buffer and Chemiluminescent Reagent.

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CPS Formulations for ELISA Applications

CPS-2 Chemiluminescent Peroxidase Substrate-2



CPS-2 Chemiluminescent Peroxidase Substrate-2

Sigma's CPS-2 can be used for the highly sensitive detection of peroxidase conjugates in a variety of ELISA. Provides a 100-fold improvement in signal to noise ratio over traditional luminol peroxidase formulations. The ultra high sensitivity combined with low background signal produces outstanding ELISA results.

Ordering Information

Product Code Product Name Quantity Technical Manual
CPS2-60 Chemiluminescent Peroxidase Substrate-2 60 ml
CPS2-120 Chemiluminescent Peroxidase Substrate-2 120 ml
CPS2-300 Chemiluminescent Peroxidase Substrate-2 300 ml

Additional Links
CPS-1 For Detection of FLAG-Tagged Proteins
3xFLAG

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Proteomics
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