Certain features of will be down for maintenance the evening of Friday August 18th starting at 8:00 pm CDT until Saturday August 19th at 12:01 pm CDT.   Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.

Enzyme Explorer


Performance without Premium Cost

Why spend more of your research dollars than you need to for an enhanced chemiluminescent (ECL) substrate? Sigma's CPS-3 Chemiluminescent Peroxidase Substrate-3 is an economic alternative for high-throughput Western applications that still outperforms the competition. This two-component system provides low nanogram detection with superior signal-to-noise ratio and less background. In addition, the two-component system is stable after mixing, so you can prepare enough working reagent for a day or a week at a time.

With CPS-3 you get:
  • Sensitivity — Low nanogram protein detection with superior signal-to-noise ratio.

  • Fast — Save time by eliminating the need to prepare fresh solutions hourly. Working solution is stable for at least 6 weeks.

  • $$ saved — CPS-3 is half the cost of the leading ECL substrate.

CPS-3 versus the Competition – Performance You Expect

Chemiluminescent detection of a FLAG®-bacterial alkaline phosphatase (BAP) fusion protein in a COS-7 cell lysate using CPS-3 and three ECL substrate formulations from the leading competitor. Sigma’s CPS-3 is more sensitive than Standard formulation B or Enhanced formulation C.

CPS-3 Chemiluminescent Peroxidase Substrate
Ordering Information
Product Code Product Name Quantity Technical Manual
CPS3-50 Chemiluminescent Peroxidase Substrate-3 50 ml
CPS3-100 Chemiluminescent Peroxidase Substrate-3 100 ml
CPS3-500 Chemiluminescent Peroxidase Substrate-3 500 ml

For your most demanding high-sensitivity Western applications, try Chemiluminescent Peroxidase Substrate-1 for Western Blotting, delivering up to 100X improvement in signal-to-noise ratio over the competition.