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Enzyme Explorer

Carbohydrate Analysis


Complex Carbohydrates
Enzyme Index
  Agarase
  Alginate Lyase
  α-Amylase
  β-Amylase
  Amyloglucosidase
  Cellulase
  Chitinase
  Chitosanase
  Chondroitinases
  Dextranase
  Driselase
  α-Glucosidase
  Hemicellulase
  Heparinases
  Hyaluronidase
  Inulinase
  Isoamylase
  Lysozyme
  Lyticase
  Laminarinase
  Pectinase
  Pectolyase
  Pectinesterase
  Pullulanase
  Xylanase
Carbohydrate Index
  Agarose
  Alginic Acid
  Amylose
  Amylopectin
  Cellulose
  Chitin
  Chitosan
  Chondroitin Sulfates
  Dextran
  β-Glucans
  Glycogen
  Hemicellulose
  Heparan Sulfate
  Heparin
  Hyaluronic Acid
  Inulin
  Lichenen
  Pectins
  Peptidoglycans
  Pullulan
  Starch
  Xylan
Kits and Reagents for Glycoprotein Analysis

Glycoprotein Analysis Manual


Agarase and Agarose

Agarose
Agarose is the principal neutral gelling component of agar extracted from algae. Agarose is a complex range of polysaccharide chains composed of alternating α-(1-3)-D-galactosyl-β-(1-4)-anhydro-L-galactosyl units.1

Agarase
EC# 3.2.1.81
Synonym: agarose 3-glycanohydrolase

Agarase catalyzes the hydrolysis of 1,3-b-D-galactosidic linkages in agarose, giving the tetramer as the predominant product. 1

from Pseudomonas atlantica
Product Number A6306 (Lyophilized, 400-800 un/mg)

References
1. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/81.html)

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Alginate Lyase and Alginic Acid

Composed of blocks of β-D-(1-4) mannuronic acid homopolymeric regions (MMMM…), α-L-(1-4)guluronic acid (GGGG…) homopolymeric regions, and alternating copolymer regions of β-D-(1-4) mannuronic acid - α-L-(1-4)-guluronic acid (GMGMGM…). Bacterial alginic acid can be acetlylated at the 2 or 3 positions on mannuronic acid.

Alginate Lyase
EC# 4.2.2.3
Synonyms: poly(β-D-manuronate) lyase

Alginate lyase cleaves at the β-(1-4)-D-mannuronic bonds residues to yield oligosaccharides with 4-deoxy-α-L-erythro-hex-4-enopyranuronosyl groups at their non-reducing terminus.

from Flavobacterium
Product Number A1603

References
1. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC4/2/2/3.html)

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Amylases, α-Glucosidase, Starches (Amylose and Amylopectin) and Glycogen



Polymers of α-(1-4)-D-glycopyranosyl units. Amylopectin has approximately 4% α-(1-6)-D-glycopyranosyl branching. Glycogen tends to have a higher frequency of branches of shorter length than amylopectin.

α-Amylase
EC# 3.2.1.1
Synonyms: 1,4-alpha-D-glucan glucanohydrolase

α-Amylase catalyzes the endohydrolysis of 1,4-α-D-glucosidic linkages in polysaccharides containing three or more 1,4-α-linked D-glucose units.

from Bacillus species
Product Number A6814 (min 400 un/mg, powder containing starch)
Product Number A7720 (min 10 Novozymes un/g, Novozymes Duramyl 300)
Product Number A6380 (1,500-3,00 un/mg, lyophilized powder)

from Bacillus amyloliquefaciens
Product Number A7595 (min 250 Novozymes un/g, Novozymes Ban 240L)

from Bacillus licheniformis
Product Number A4551 (1,000-1,500 un/mg, lyophilized powder)
Product Number A3403 (500-1,000 un/mg, Novozymes Termamyl 120, heat –stable solution)
Product Number A3403 (component for starch assay kit)

from Aspergillus oryzae
Product Number A6211 (150-250 un/mg, lyophilized powder)
Product Number A8220A8220 (min 0.8 Novozymes un/g, Novozymes Fungamyl 800L)]

from Barley malt
Product Number A2771 (min 1 un/mg, lyophilized powder containing lactose)

from Porcine pancreas
Product Number A3176 (10-30 un/mg, lyophilized powder containing lactose)
Product Number A6255 (700-1,400 un/mg, NaCl suspension, DFP treated)
Product Number A2643 (min 500 un/mg, Ammonium Sulfate suspension, DFP treated)
Product Number A4268A4268 (700-1,400 un/mg, NaCl suspension, PMSF treated)

from Human pancreas
Product Number A9972 (min 100 un/mg, lyophilized powder containing TBS)

from Human saliva
Product Number A1031 (300-1,000 un/mg, lyophilized powder)
Product Number A0521 (1,000-1,500 un/mg, lyophilized powder)

References
1. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/1.html/)

β-Amylase
EC# 3.2.1.2
Synonyms: 1,4-α-D-glucan maltohydrolase

β-Amylase catalyzes the exo-hydrolysis of 1,4-α-D-glucosidic linkages in polysaccharides resulting in the successive liberation of maltose units from the non-reducing ends of the chains.

from Barley
Product Number A7130 (20-80 un/mg, lyophilized powder)

from Sweet potato
Product Number A7005 (750-1,000 un/mg, ammonium sulfates suspension)

References
1. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/2.html/

Amyloglucosidase
EC# 3.2.1.3
Synonyms: glucan 1,4-α-glucosidase, glucoamylase, γ-amylase

Amyloglucosidase catalyzes the hydrolysis of terminal 1,4-linked α-D-glucose residues successively from the non-reducing ends of maltooligo- and polysacharides with release of β-D-glucose. Most forms of the enzyme can rapidly hydrolyze 1,6-α-D-glucosidic bonds when the next bond in the sequence is 1,4- and some preparations of this enzyme hydrolyze 1,6- and 1,3-α-D-glucosidic bonds in other polysaccharides.

from Aspergillus niger
Product Number A7420 (30-60 un/mg, lyophilized powder)
Product Number A7095 (min 300 Novozymes un/ml, solution, Novozymes AMG 300L)
Product Number A3042 (min 5,000 un/ml, solution in 1M glucose)
Product Number A1602 (min 40 un/mg, ammonium sulfate suspension)

from Candida tsukubaensis
Product Number A2330 (50-150 un/mg, ammonium sulfate suspension)

from Rhizopus species
Product Number A7255 (min 5,000 un/g, stabilized powder)

References
1. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/3.html/)

α-Glucosidase
EC# 3.2.1.20
Synonyms: maltase

α-Glucosidase catalyzes the hydrolysis of terminal 1,4-linked α-D-glucose residues successively from the non-reducing ends of maltooligo- and to a lesser extent polysacharides with release of β-D-glucose. Most forms of the enzyme can slowly hydrolyze 1,6-α-D-glucosidic bonds.

from Bacillus stearothermophilus
Product Number G3651 (min 50 un/mg, lyophilized powder)

from Saccharomyces cerevisiea
Product Number G0660 (min 125 un/mg, recombinant, lyophilized powder)
Product Number G5003 (min 10 un/mg, lyophilized powder)

from Rice
Product Number G9259 (40-80 un/mg, ammonium sulfate suspension)

References
1. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/20.html/)

Isoamylase
EC# 3.2.1.68
Synonyms: glycogen a-1,6-glucanohydrolase, debranching enzyme

from Pseudomonas sp.
Product Number I5284 (suspension, min. 3,000,000 un/mg)

Isoamylase catalyzes the hydrolysis of (1-6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins.

References
1. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/68.html/)

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Cellulase, Lyticase, Laminanrinase, Cellulose, β-Glucans and Lichenen

Repeating linear polymer of two β-(1-4)-D-glycopyranosyl and one β-(1-3)-D-glycopyranosyl unit.


Polymer of β-(1-4)-D-glycopyranosyl units occurring as predominantly as cellotriose and cellotetraose separated by single β-(1-3)-D-glycopyranosyl units. Cross-linking can occur within the consecutive cellotriose regions.


Polymer of β-(1-3)-D-glycopyranosyl units with branching at β-(1-6)-D-glycopyranosyl units.


Cellulase
EC# 3.2.1.4
Synonyms: 1,4-(1,3;1,4)-β-D-glucan 4-glucanohydrolase

from Aspergillus species
Product Number C2605 (Novozymes Carezyme 1000 L)

from Aspergillus niger
Product Number C1184 (powder, min. 0.3 un/mg)

from Trichoderma reesei
Product Number C8546 (powder, min. 1.0 un/mg)
Product Number C2730 (Novozymes Celluclast 1.5 L)

from Trichoderma viride
Product Number C0615 (Yakult Onozuka RS)
Product Number C1794 (cell culture tested, powder, min. 3.0-10 un/mg)
Product Number C9422 (powder, min. 3.0-10 un/mg)

Cellulase catalyzes the endohydrolysis of 1,4-β-D-glucosidic linkages in cellulose, lichenin and cereal β-D-glucans1

Cellobiohydrolase I
from Hypocrea jecorina.
Product Number E6412 (recombinant expressed in corn)

Driselase
from Basidiomycetes sp.
Product Number D9515 (Crude powder containing laminarinase, xylanase and cellulase)

Laminarinase
EC# 3.2.1.6
Synonyms: endo-1,3(4)-β-glucanase

Laminarinase catalyzes the endohydrolysis of 1,3- or 1,4-linkages in β-D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolysed is itself substituted at C-3.

from Penicillium Species
Product Number L9295 (lyophilized powder, 5-10 un/mg)

from Trichoderma Species
Product Number L5272 (lyophilized powder, 100-400 un/g)

Lyticase
Synonyms: Zymolyase
from Arthrobacter luteus
Product Number L2524 (lyophilized powder, min 2,000 un/mg protein)
Product Number L4025 (lyophilized powder, min 200 un/mg solid)
Product Number L5263 (lyophilized powder, min 4,000 un/mg protein)

from over expressing E. coli
Product Number L4276 (500 un/vial)

References
1. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/4.html)
2. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/6.html)

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