Carbohydrate Analysis

Agarose
Agarose is the principal neutral gelling component of agar extracted from algae. Agarose is a complex range of polysaccharide chains composed of alternating α-(1-3)-D-galactosyl-β-(1-4)-anhydro-L-galactosyl units.¹

Agarase
EC# 3.2.1.81
Synonym: agarose 3-glycanohydrolase

Agarase catalyzes the hydrolysis of 1,3-b-D-galactosidic linkages in agarose, giving the tetramer as the predominant product. ¹

from Pseudomonas atlantica
Product Number A6306 (Lyophilized, 400-800 un/mg)

References
1. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/81.html)

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Composed of blocks of β-D-(1-4) mannuronic acid homopolymeric regions (MMMM…), α-L-(1-4)guluronic acid (GGGG…) homopolymeric regions, and alternating copolymer regions of β-D-(1-4) mannuronic acid - α-L-(1-4)-guluronic acid (GMGMGM…). Bacterial alginic acid can be acetlylated at the 2 or 3 positions on mannuronic acid.

Alginate Lyase
EC# 4.2.2.3
Synonyms: poly(β-D-manuronate) lyase

Alginate lyase cleaves at the β-(1-4)-D-mannuronic bonds residues to yield oligosaccharides with 4-deoxy-α-L-erythro-hex-4-enopyranuronosyl groups at their non-reducing terminus.

from Flavobacterium
Product Number A1603

References
1. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC4/2/2/3.html)

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Polymers of α-(1-4)-D-glycopyranosyl units. Amylopectin has approximately 4% α-(1-6)-D-glycopyranosyl branching. Glycogen tends to have a higher frequency of branches of shorter length than amylopectin.

α-Amylase
EC# 3.2.1.1
Synonyms: 1,4-alpha-D-glucan glucanohydrolase

α-Amylase catalyzes the endohydrolysis of 1,4-α-D-glucosidic linkages in polysaccharides containing three or more 1,4-α-linked D-glucose units.

from Bacillus species
Product Number A6814 (min 400 un/mg, powder containing starch)
Product Number A7720 (min 10 Novozymes un/g, Novozymes Duramyl 300)
Product Number A6380 (1,500-3,00 un/mg, lyophilized powder)

from Bacillus amyloliquefaciens
Product Number A7595 (min 250 Novozymes un/g, Novozymes Ban 240L)

from Bacillus licheniformis
Product Number A4551 (1,000-1,500 un/mg, lyophilized powder)
Product Number A3403 (500-1,000 un/mg, Novozymes Termamyl 120, heat –stable solution)
Product Number A3403 (component for starch assay kit)

from Aspergillus oryzae
Product Number A6211 (150-250 un/mg, lyophilized powder)
Product Number A8220A8220 (min 0.8 Novozymes un/g, Novozymes Fungamyl 800L)]

from Barley malt
Product Number A2771 (min 1 un/mg, lyophilized powder containing lactose)

from Porcine pancreas
Product Number A3176 (10-30 un/mg, lyophilized powder containing lactose)
Product Number A6255 (700-1,400 un/mg, NaCl suspension, DFP treated)
Product Number A2643 (min 500 un/mg, Ammonium Sulfate suspension, DFP treated)
Product Number A4268A4268 (700-1,400 un/mg, NaCl suspension, PMSF treated)

from Human pancreas
Product Number A9972 (min 100 un/mg, lyophilized powder containing TBS)

from Human saliva
Product Number A1031 (300-1,000 un/mg, lyophilized powder)
Product Number A0521 (1,000-1,500 un/mg, lyophilized powder)

References
1. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/1.html/)

β-Amylase
EC# 3.2.1.2
Synonyms: 1,4-α-D-glucan maltohydrolase

β-Amylase catalyzes the exo-hydrolysis of 1,4-α-D-glucosidic linkages in polysaccharides resulting in the successive liberation of maltose units from the non-reducing ends of the chains.

from Barley
Product Number A7130 (20-80 un/mg, lyophilized powder)

from Sweet potato
Product Number A7005 (750-1,000 un/mg, ammonium sulfates suspension)

References
1. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/2.html/

Amyloglucosidase
EC# 3.2.1.3
Synonyms: glucan 1,4-α-glucosidase, glucoamylase, γ-amylase

Amyloglucosidase catalyzes the hydrolysis of terminal 1,4-linked α-D-glucose residues successively from the non-reducing ends of maltooligo- and polysacharides with release of β-D-glucose. Most forms of the enzyme can rapidly hydrolyze 1,6-α-D-glucosidic bonds when the next bond in the sequence is 1,4- and some preparations of this enzyme hydrolyze 1,6- and 1,3-α-D-glucosidic bonds in other polysaccharides.

from Aspergillus niger
Product Number A7420 (30-60 un/mg, lyophilized powder)
Product Number A7095 (min 300 Novozymes un/ml, solution, Novozymes AMG 300L)
Product Number A3042 (min 5,000 un/ml, solution in 1M glucose)
Product Number A1602 (min 40 un/mg, ammonium sulfate suspension)

from Candida tsukubaensis
Product Number A2330 (50-150 un/mg, ammonium sulfate suspension)

from Rhizopus species
Product Number A7255 (min 5,000 un/g, stabilized powder)

References
1. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/3.html/)

α-Glucosidase
EC# 3.2.1.20
Synonyms: maltase

α-Glucosidase catalyzes the hydrolysis of terminal 1,4-linked α-D-glucose residues successively from the non-reducing ends of maltooligo- and to a lesser extent polysacharides with release of β-D-glucose. Most forms of the enzyme can slowly hydrolyze 1,6-α-D-glucosidic bonds.

from Bacillus stearothermophilus
Product Number G3651 (min 50 un/mg, lyophilized powder)

from Saccharomyces cerevisiea
Product Number G0660 (min 125 un/mg, recombinant, lyophilized powder)
Product Number G5003 (min 10 un/mg, lyophilized powder)

from Rice
Product Number G9259 (40-80 un/mg, ammonium sulfate suspension)

References
1. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/20.html/)

Isoamylase
EC# 3.2.1.68
Synonyms: glycogen a-1,6-glucanohydrolase, debranching enzyme

from Pseudomonas sp.
Product Number I5284 (suspension, min. 3,000,000 un/mg)

Isoamylase catalyzes the hydrolysis of (1-6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins.

References
1. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/68.html/)

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Repeating linear polymer of two β-(1-4)-D-glycopyranosyl and one β-(1-3)-D-glycopyranosyl unit.

Polymer of β-(1-4)-D-glycopyranosyl units occurring as predominantly as cellotriose and cellotetraose separated by single β-(1-3)-D-glycopyranosyl units. Cross-linking can occur within the consecutive cellotriose regions.

Polymer of β-(1-3)-D-glycopyranosyl units with branching at β-(1-6)-D-glycopyranosyl units.

Cellulase
EC# 3.2.1.4
Synonyms: 1,4-(1,3;1,4)-β-D-glucan 4-glucanohydrolase


from Aspergillus species
Product Number C2605 (Novozymes Carezyme 1000 L)

from Aspergillus niger
Product Number C1184 (powder, min. 0.3 un/mg)

from Trichoderma reesei
Product Number C8546 (powder, min. 1.0 un/mg)
Product Number C2730 (Novozymes Celluclast 1.5 L)

from Trichoderma viride
Product Number C0615 (Yakult Onozuka RS)
Product Number C1794 (cell culture tested, powder, min. 3.0-10 un/mg)
Product Number C9422 (powder, min. 3.0-10 un/mg)

Cellulase catalyzes the endohydrolysis of 1,4-β-D-glucosidic linkages in cellulose, lichenin and cereal β-D-glucans¹

Driselase
from Basidiomycetes sp.
Product Number D9515 (Crude powder containing laminarinase, xylanase and cellulase)

Laminarinase
EC# 3.2.1.6
Synonyms: endo-1,3(4)-β-glucanase

Laminarinase catalyzes the endohydrolysis of 1,3- or 1,4-linkages in β-D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolysed is itself substituted at C-3.

from Penicillium Species
Product Number L9295 (lyophilized powder, 5-10 un/mg)

from Trichoderma Species
Product Number L5272 (lyophilized powder, 100-400 un/g)

Lyticase
Synonyms: Zymolyase
from Arthrobacter luteus
Product Number L2524 (lyophilized powder, min 2,000 un/mg protein)
Product Number L4025 (lyophilized powder, min 200 un/mg solid)
Product Number L5263 (lyophilized powder, min 4,000 un/mg protein)

from over expressing E. coli
Product Number L4276 (500 un/vial)

References
1. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/4.html)
2. Enzyme Nomenclature (www.chem.qmul.ac.uk/iubmb/enzyme/EC3/2/1/6.html)

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