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Enzyme Explorer
Cell Detachment
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Select the ideal enzyme for your cell detachment or tissue dissociation needs with these helpful links. Learn about the factors that influence your tissue digestion-dissociation and discover optimal conditions for working with these enzymes.
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Collagenase
Because of its unique ability to hydrolyze native collagen, it is widely used to isolate cells from animal tissue.
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Hyaluronidase
Catalyzes the random hydrolysis of
1,4-β-D-glycosidic linkages.
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Papain
Relatively nonspecific sulfhydryl protease that can be used alone for dissociation.
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Elastase
Useful for the dissociation of tissues containing higher amounts of elastin.
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DNase
Reduces viscosity from DNA released from damaged cells during harvesting.
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Protease Type XIV
Extremely nonspecific protease for dissociation of various tissues.
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Trypsin
Serine protease commonly used for cell detachment and tissue dissociation.
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