|
Chymase, E.C. 3.4.21.39
| Product Name |
Product # |
| Chymase |
C9612 |
| |
Specificity:
|
|
Preferential cleavage: Phe-Xaa > Tyr-Xaa > Trp-Xaa > Leu-Xaa
|
| |
Synthethic Substrates:
|
|
BTEE, B6125
|
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Chymotrypsin, a-, E.C. 3.4.21.1
| Product Name |
Product # |
| a-Chymotrypsin from bovine pancreas, suitable for protein sequencing Salt-free, lyophilized powder |
C6423 |
| b-Chymotrypsin from bovine pancreas |
C4629 |
| a-Chymotrypsin Insoluble enzyme attached to carboxymethyl cellulose from bovine pancreas |
C7260 |
| a-Chymotrypsin-Agarose from bovine pancreas |
C9134 |
| a-Chymotrypsin DFP treated |
C1012 |
| a-Chymotrypsin from human pancreas, Lyophilized powder |
C8946 |
| a-Chymotrypsin from bovine pancreas, Type II Essentially salt-free, lyophilized powder >40 units/mg protein |
C4129 |
| a-Chymotrypsin from bovine pancreas, Type I-S Essentially salt-free, lyophilized powder 40-60 units/mg protein |
C7762 |
| a-Chymotrypsin TLCK Treated from bovine pancreas |
C3142 |
| |
Specificity:
|
|
Preferential cleavage: Ty-Xaa, Trp-Xaa, Phe-Xaa, Leu-Xaa
|
| |
Synthethic Substrates:
|
|
N-Succinyl-Ala-Ala-Pro-Phe p-nitroanilide, S7388
N-Succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin, S9761
Ala-Ala-Phe-7-amido-4-methylcoumarin, A3401
ALA-ALA-VAL-ALA P-Nitroanilide, A9273
BTEE, B6125
N-Benzoyl-L-tyrosine p-nitroanilide , B6760
Glutaryl-Ala-Ala-Phe-4-methoxy-b-naphthylamide, G3769
N-Glutaryl-Gly-Gly-Phe-b-naphthylamide, G4636
4-Methylumbelliferyl p-trimethylammoniocinnamate chloride, M4507
N-Succinyl-Gly-Gly-Phe p-nitroanilide, S1899
N-Succinyl-L-phenylalanine p-nitroanilide, S2628
N-Succinyl-Ala-Ala-Pro-Phe 7-amido-4-trifluoromethylcoumarin, S5189
N-Succinyl-Ala-Ala-Pro-Phe 4-methoxy-2-naphthylamide, S5314
|
| |
Inhibitors:
|
|
Z-L-Phe chloromethyl ketone, C9511
a2-Antiplasmin, A0914
Aprotinin, A1153
e-Amino-n-caproic acid, A2504
a1-Antitrypsin, A6150
e-Amino-n-caproic acid, A7824
4-(2-Aminoethyl) benzenesulfonyl fluoride hydrochloride, A8456
a1-Antitrypsin, A9024
a1-Antchymotrypsin, A9285
Bromoenol lactone, B1552
Chymostatin, C7268
Diisopropyl fluorophosphate, D0879
Ecotin, E1398
N-Acetyl-eglin C, E7888
Gabexate mesylate, G2417
Leupeptin, L2023
Trypsin Inhibitor, T0256
Trypsin Inhibitor, T1886
N-Tosyl-L-phenylalanine chloromethyl ketone, T4376
Trypsin Inhibitor, T4385
Trypsin Inhibitor, T9253
Trypsin Inhibitor, T9378
Trypsin-chymotrypsin inhibitor, T9777
|
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Clostripain, E.C. 3.4.22.8
| Product Name |
Product # |
| Clostripain from Clostridium histolyticum |
C0888 |
| |
Specificity:
|
|
Preferential cleavage: Arg-Xaa , including Arg-Pro, but not Lys-
|
| |
Synthethic Substrates:
|
|
BAEE, B4500
|
| |
Inhibitors:
|
|
p-Chloromercuribenzoic acid Free Acid, C5913
Histatin 5, H6027
N a-p-Tosyl-L-lysine chloromethyl ketone hydrochloride, T7254
|
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Collagenase, E.C. 3.4.24.3
| Product Name |
Product # |
| Collagenase from Clostridium histolyticum, 2-5 FALGPA units/mg solid >1200 CDU/mg solid (CDU = collagen digestion units) Type XI |
C7657 |
| Collagenase from Clostridium histolyticumType VII powder 1000-3000 CDU/mg solid (CDU = collagen digestion units) 4-12 FALGPA units/mg protein cell culture, tested |
C2799 |
| Collagenase from Clostridium histolyticum, cell culture, tested Lyophilized powder Type XI-S |
C9697 |
| Collagenase from Clostridium histolyticum, Type XI powder >1200 CDU/mg solid (CDU = collagen digestion units) 2-5 FALGPA units/mg protein cell culture, tested |
C9407 |
| Collagenase/Dispase from Achromobacter iophagus/Bacillus polymyxa |
C3180 |
| Collagenase from Clostridium histolyticum, Type I-A powder >125 CDU/mg solid (CDU = collagen digestion units) 0.5-2.0 FALGPA units/mg protein cell culture, tested |
C2674 |
| Collagenase from Clostridium histolyticum, Type VII-S cell culture, tested Lyophilized powder |
C9572 |
| Collagenase from Clostridium histolyticum, Type III Fraction A Lyophilized powder 2-10 FALGPA units/mg protein minimum 400 CDU/mg solid (CDU = collagen digestion units) |
C0255 |
| Collagenase from Clostridium histolyticum, Type VII Lyophilized powder 1,000-3,000 CDU/mg solid (CDU = collagen digestion units) 4-12 FALGPA units/mg protein |
C0773 |
| Collagenase from Clostridium histolyticum, 1.1-1.5 FALGPA units/mg protein (Neutral Protease: 20-50units per mg solid) Sigma Blend Type H |
C8051 |
| Collagenase from Clostridium histolyticum, 1-3 FALGPA units/mg protein >125 CDU/mg solid (CDU = collagen digestion units) Type V |
C9263 |
| Collagenase from Clostridium histolyticum, 1.8-2.2 FALGPA units/mg protein (Neutral Protease:<10 units per mg solid) Sigma Blend Type F |
C7926 |
| Collagenase from Clostridium histolyticum, 0.5-0.9 FALGPA units/mg protein (Neutral Protease: 50-80 units per mg solid) Sigma Blend Type L |
C8176 |
| Collagenase + protease inhibitor, suitable (Each lot is evaluated for suitability in the release of physiologically active rat pancreatic islets (for methodology see Type V)) 2-5 FALGPA units/mg protein minimum |
C6079 |
| Collagenase from Clostridium histolyticum, Essentially salt-free, lyophilized powder 0.5-3.0 FALGPA units/mg protein >125 CDU/mg solid (CDU = collagen digestion units) Type IA |
C9891 |
| Collagenase from Clostridium histolyticum, Essentially salt-free, lyophilized powder 0.25-1.0 FALGPA units/mg protein >125 CDU/mg solid (CDU = collagen digestion units) Type I |
C0130 |
| Collagenase from Clostridium histolyticum, suitable for release of rat epididymal adipocytes and hepatocytes (for methodology see Type II and Type IV) 0.5-3.0 FALGPA units/mg solid >125 CDU/mg solid (CDU = collagen digestion units) Type VIII |
C2139 |
| Collagenase from Clostridium histolyticum, 0.5-3.0 FALGPA units/mg solid >125 CDU/mg solid (CDU = collagen digestion units) Type II |
C6885 |
| Collagenase from Clostridium histolyticum, 0.5-3.0 FALGPA units/mg solid >125 CDU/mg solid (CDU = collagen digestion units) Type IV |
C5138 |
| Collagenase from Clostridium histolyticum, Type I-AS cell culture, tested Lyophilized powder |
C9722 |
| |
Specificity:
|
|
Digestion of native collagen in the triple helical region at Gly bonds. With synthetic peptides, a preference is shown for Gly at P3 and P1'; Pro and Ala at P2 and P2'; and hydroxyproline, Ala or Arg at P3'
|
| |
Synthethic Substrates:
|
|
Z-Gly-Pro-Leu-Gly-Pro, C0627
Collagen Type I-FITC, C4361
Collagen Type II-FITC, C4486
Collagen-Fluorescein, C5858
Collagen-Fluorescein, C8008
N-(2,4-Dinitrophenyl)-Pro-Leu-Gly-Cys(Me)-His-Ala-D-Arg amide, D0296
FALGPA, F5135
|
| |
Inhibitors:
|
|
5-(R,S)-T-trans-Cinnamido-7-methyl-4-oxo-octanoyl-L-prolyl-L-proline, C0360
Dichloromethylenediphosphonic acid disodium salt, D4434
Ecotin, E1398
EDTA, ED2SS
Isoamylphosphonyl-Gly-L-Pro-L-Ala, dipotassium, I7029
(2R)-2-Mercaptomethyl-4-methylpentanoyl-beta-(2-naphthyl)-Ala-Ala Amide, M3531
|
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Complement C1r, E.C. 3.4.21.41
| Product Name |
Product # |
| Complement C1r from human serum |
C2162 |
| |
Specificity:
|
|
Selective cleavage of Lys(or Arg) Ile bond in complement subcomponent C1s
|
| |
Inhibitors:
|
|
Complement C1 esterase inhibitor, C2412
Diisopropyl fluorophosphate, D0879
C1 Esterase inhibitor, E0518
|
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Complement C1s, E.C. 3.4.21.42
| |
Specificity:
|
|
Cleavage of Arg-Ala bond in complement component C4 to form C4a and C4b, and Lys(or Arg) Lys bond in complement component C2 to form C2a and C2b: the "classical" pathway C3 convertase
|
| |
Inhibitors:
|
|
Complement C1 esterase inhibitor, C2412
Diisopropyl fluorophosphate, D0879
C1 Esterase inhibitor, E0518
|
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Complement Factor D, E.C. 3.4.21.46
| Product Name |
Product # |
| Complement factor D from human plasma |
C5688 |
| |
Specificity:
|
|
Selective cleavage of Arg-Lys bond in complement factor B when in complex with complement subcomponent C3b or with cobra venom factor
|
| |
Inhibitors:
|
|
Diisopropyl fluorophosphate, D0879
|
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Complement factor I, E.C. 3.4.21.45
| Product Name |
Product # |
| Complement factor I from human plasma |
C5938 |
| |
Specificity:
|
|
Inactivates complement subcomponents C3b, iC3b and C4b by proteolytic cleavage
|
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Cucumisin, E.C. 3.4.21.25
| Product Name |
Product # |
| Cucumisin |
C1932 |
| |
Specificity:
|
|
Hydrolysis of proteins with broad specificity
|
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Dipeptidyl peptidase IV, E.C. 3.4.14.5
| Product Name |
Product # |
| Dipeptidyl Peptidase from porcine kidney |
D7052 |
| |
Specificity:
|
|
Release of an N-terminal dipeptide, Xaa-Xbb Xcc-, from a polypeptide, preferentially when Xbb is Pro, provided Xcc is neither Pro nor hydroxyproline
|
| |
Synthethic Substrates:
|
|
Gly-Pro-7-amido-4-methylcoumarin hydrobromide, G2761
Gly-Pro p-nitroanilide hydrochloride, G0513
|
| |
Inhibitors:
|
|
Ile-Pro-Ile, I9759
|
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Elastase, leukocyte, E.C. 3.4.21.37
| Product Name |
Product # |
| Elastase from human leukocytes |
E8140 |
| |
Specificity:
|
|
Hydrolysis of proteins, including elastin. Preferential cleavage Val-Xaa > Ala-Xaa
|
| |
Synthethic Substrates:
|
|
Boc-Ala-Pro-Nva p-chlorothiobenzyl ester, B2017
N-Methoxysuccinyl-Ala-Ala-Pro-Val-7-amido-4-trifluoromethylcoumar, M4563
N-(Methoxysuccinyl)-Ala-Ala-Pro-Val 4-nitroanilide, M4765
N-Methoxysuccinyl-Ala-Ala-Pro-Val-7-amido-4-methylcoumarin, M9771
|
| |
Inhibitors:
|
|
Aprotinin, A1153
Diisopropyl fluorophosphate, D0879
3,4-Dichloroisocoumarin, D7910
Eglin C fragment 60-63 methyl ester, E2017
N-Acetyl-eglin C, E7888
a2-Macroglobulin, M6159
Phenylmethylsulfonyl fluoride, P7626
|
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Elastase, pancreatic, E.C. 3.4.21.36
| Product Name |
Product # |
| Elastase, pancreatic solution from porcine pancreas, Type I Aqueous suspension minimum 4 units/mg protein |
E1250 |
| Elastase, pancreatic from porcine pancreas, Lyophilized powder 3-6 units/mg protein (biuret) cell culture, tested |
E7885 |
| Elastase, pancreatic from porcine pancreas, Type IV Lyophilized powder minimum 4 units/mg protein (biuret) |
E0258 |
| Elastase, pancreatic from porcine pancreas, Type II-A Lyophilized powder minimum 1 units/mg protein (biuret) |
E6883 |
| Elastase, pancreatic from porcine pancreas, Type III Lyophilized powder minimum 4 units/mg protein |
E0127 |
| |
Specificity:
|
|
Hydrolysis of proteins, including elastin. Preferential cleavage: Ala-Xaa
|
| |
Synthethic Substrates:
|
|
N-Methoxysuccinyl-Ala-Ala-Pro-Val-7-amido-4-methylcoumarin, M9771
|
| |
Inhibitors:
|
|
a1-Antitrypsin, A6150
a1-Antitrypsin, A9024
Diisopropyl fluorophosphate, D0879
3,4-Dichloroisocoumarin, D7910
Ecotin, E1398
a2-Macroglobulin, M6159
|
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Endoproteinase Arg-C, E.C. 3.4.21.35
| Product Name |
Product # |
| Endoproteinase Arg-C murine submaxillary gland, suitable for protein sequencing Lyophilized powder |
P6056 |
| Endoproteinase Arg-C murine submaxillary gland, Type XX-S Lyophilized powder 250-800 units/mg protein (Lowry) |
P5171 |
| |
Specificity:
|
|
Cleaves specifically at the carboxyl side of arginine residues. The enzyme has also been shown to cleave Lys-Lys and Lys-Arg bonds; all Arg-X bonds may not be hydrolyzed. This enzyme also exhibits esterase and amidase activities.
|
| |
Synthethic Substrates:
|
|
TAME, T4626
|
| |
Inhibitors:
|
|
Diisopropyl fluorophosphate, D0879
a2-Macroglobulin, M6159
N-a-p-Tosyl-L-lysine chloromethyl ketone Hydrochloride, T7254
|
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Endoproteinase Asp-N, E.C. 3.4.24.33
| Product Name |
Product # |
| Endoproteinase Asp-N from Pseudomonas fragi mutant strain |
P3303 |
| |
Specificity:
|
|
Cleavage of Xaa-Asp, Xaa-Glu and Xaa-cysteic acid bonds
|
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Endoproteinase Glu-C, E.C. 3.4.21.19
| Product Name |
Product # |
| Endoproteinase Glu-C Staphylococcus aureus V8, suitable for protein sequencing Lyophilized powder |
P6181 |
| Endoproteinase Glu-C Staphylococcus aureus V8, Type XVII-B Lyophilized powder 500-1,000 units/mg solid |
P2922 |
| |
Specificity:
|
|
Preferential cleavage: Glu-Xaa, Asp-Xaa
|
| |
Inhibitors:
|
|
Diisopropyl fluorophosphate, D0879
a2-Macroglobulin, M6159
|
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Endoproteinase Lys-C, E.C. 3.4.21.50
| Product Name |
Product # |
| Endoproteinase Lys-C from Lysobacter enzymogenes, suitable for protein sequencing Lyophilized powder |
P3428 |
| Endoproteinase Lys-C from Lysobacter enzymogenes, Lyophilized powder |
P2289 |
| |
Specificity:
|
|
Cleaves at the carboxyl side of lysyl residues. Lys-Pro and Lys-Glu bonds are also cleaved
|
| |
Synthethic Substrates:
|
|
N-(p-Tosyl)-Gly-Pro-Lys 4-nitroanilide acetate salt, T6140
|
| |
Inhibitors:
|
|
Aprotinin, A1153
Leupeptin, L2023
Diisopropyl fluorophosphate, D0879
N-a-p-Tosyl-L-lysine chloromethyl ketone Hydrochloride, T7254
|
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Enterokinase, E.C. 3.4.21.9
| Product Name |
Product # |
| Enterokinase from bovine intestine, powder |
E5144 |
| Enterokinase porcine intestine, Lyophilized powder 100-500 units/mg protein |
E0885 |
| Enterokinase from bovine intestine, Salt-free, lyophilized powder >0.5 units/mg solid |
E5510 |
| Enterokinase porcine intestine, Salt-free, lyophilized powder approx. 2 units/mg solid |
E0632 |
| Enterokinase solution from calf intestine, Buffered aqueous glycerol solution minimum 1,000 units/mg protein (BCA) |
E0766 |
| |
Specificity:
|
|
Activation of trypsinogen by selective cleavage of Lys6-Ile bond
|
| |
Synthethic Substrates:
|
|
Gly-Asp-Asp-Asp-Asp-Lys-b-naphthylamide, G5261
|
| |
Inhibitors:
|
|
Diisopropyl fluorophosphate, D0879
a2-Macroglobulin, M6159
|
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Factor Xa, E.C. 3.4.21.6
| Product Name |
Product # |
| Factor X Activated (Xa) bovine plasma |
F2027 |
| |
Specificity:
|
|
Selective cleavage of Arg-Thr and then Arg-Ile bonds in prothrombin to form thrombin
|
| |
Synthethic Substrates:
|
|
N-Benzoyl-Ile-Glu-Gly-Arg p-nitroanilide hydrochloride, B2291
N-Benzoyl-Ile-Glu-Gly-Arg p-nitroanilide acetate salt, B7020
Boc-Ile-Glu-Gly-Arg-7-amido-4-methylcoumarin hydrochloride, B9760
Factor Xa chromogenic substrate, F3301
4-Methylumbelliferyl 4-guanidinobenzoate hydrochloride monohydrate, M4149
|
| |
Inhibitors:
|
|
a2-Antiplasmin, A0914
Antithrombin III, Human, A2221
Antithrombin III, Rat, A6397
Antithrombin III, Bovine, A9141
Diisopropyl fluorophosphate, D0879
3,4-Dichloroisocoumarin, D7910
Ecotin, E1398
Phenylmethylsulfonyl fluoride, P7626
Trypsin Inhibitor, T9003
|
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|
