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Enzyme Explorer

Proteolytic Enzymes V

Plasmin, E.C. 3.4.21.7

Product Name
Product # 
Plasmin from porcine blood P8644
 

Specificity:

 

Preferential cleavage: Lys-Xaa > Arg-Xaa; higher selectivity than trypsin. Converts fibrin into soluble products

 

Synthethic Substrates:

 

D-Ala-Leu-Lys-7-amido-4-methylcoumarin, A8171
Boc-Val-Leu-Lys-7-amido-4-methylcoumarin, B1136
Z-Ala-Ala-Lys 4-methoxy-b-naphthylamide formate salt, C2147
Gly-Arg-p-nitroanilide dihydrochl, G8148
D-Ile-Phe-Lys p-nitroanilide, I6886
N-(p-Tosyl)-Gly-Pro-Lys 4-nitro, T6140
D-Val-Leu-Lys 4-nitroanilide dihydrochloride, V0882

 

Inhibitors:

 

e-Amino-n-caproic acid, A2504
e-Amino-n-caproic acid, A7824
4-(2-Aminoethyl) benzenesulfonyl fluoride hydrochloride, A8456
Antipain, A6191
a2-Antiplasmin, A0914
Antithrombin III, Human, A2221
Antithrombin III, Bovine, A9141
Aprotinin, A1153
Bdellin, B3906
Complement C1 esterase inhibitor, C2412
Complement C1 esterase inhibitor, E0518
3,4-Dichloroisocoumarin, D7910
Diisopropyl fluorophosphate, D0879
Elastatinal, E0881
Gabexate mesylate, G2417
Leupeptin, L2023
a2-Macroglobulin, M6159
Phenylmethylsulfonyl fluoride, P7626
Urinary trypsin inhibitor fragment, U4751

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Prolidase, E.C. 3.4.13.9

Product Name
Product # 
Prolidase porcine kidney P6675
 

Specificity:

 

Hydrolysis of Xaa Pro dipeptides; also acts on aminoacyl-hydroxyproline analogs. No action on Pro-Pro

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Pronase E

Product Name
Product # 
Protease Streptomyces griseus, powder Type XIV approx. 4 units/mg solid P5147
Protease Streptomyces griseus P6911
Protease Streptomyces griseus, powder approx. 4 units/mg solid Embryo, tested P8811
 

Specificity:

 

Nonspecific Protease mixture

 

Inhibitors:

 

Diisopropyl fluorophosphate, D0879
EDTA, ED2SS

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Prostate Specific Antigen, E.C. 3.4.21.77

Product Name
Product # 
Prostate specific antigen from Human Semen, minimum 95% (SDS-PAGE) Buffered aqueous solution P3338
Prostate specific antigen from Human Semen, minimum 99% (SDS-PAGE) Buffered aqueous solution P3235
 

Specificity:

 

Preferential cleavage: Tyr-Xaa

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Protease, Alkalophilic from Streptomyces griseus

Product Name
Product # 
Protease Streptomyces griseus P0652
 

Specificity:

 

Broad specificity industrial enzyme preparation

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Protease from Aspergillus (Flavourzyme), E.C. 3.4.11.1

Product Name
Product # 
Protease from Aspergillus oryzae P6110
 

Specificity:

 

Broad specificity industrial enzyme preparation

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Protease from Aspergillus saitoi (Molsin)

Product Name
Product # 
Protease from Aspergillus saitoi P2143
 

Specificity:

 

Broad specificity industrial enzyme preparation

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Protease from Aspergillus sojae (Protease IP)

Product Name
Product # 
Protease from Aspergillus sojae P7026
 

Specificity:

 

Broad specificity industrial enzyme preparation

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Protease from Bacillus licheniformis (alkaline protease)

Product Name
Product # 
Protease Bacillus licheniformis P5459
 

Specificity:

 

Broad specificity industrial enzyme preparation

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Protease from Bacillus licheniformis (Alcalase)

Product Name
Product # 
Protease Bacillus licheniformis P4860
 

Specificity:

 

Broad specificity industrial enzyme preparation

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Protease from Bacillus polymyxa

Product Name
Product # 
Protease from Bacillus polymyxa P6141
 

Specificity:

 

Broad specificity, used for cell dissociation

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Protease from Bacillus sp (Savinase)

Product Name
Product # 
Protease Bacillus sp. P3111
 

Specificity:

 

Broad specificity industrial enzyme preparation

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Protease from Bacillus sp (Esperase)

 

Specificity:

 

Broad specificity industrial enzyme preparation

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Protease from Rhizopus sp.

 

Specificity:

 

Broad specificity industrial enzyme preparation

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Protease S

Product Name
Product # 
Protease S Pyrococcus furiosus P6361
 

Specificity:

 

Broad specificity thermostable protease

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Proteasomes

Product Name
Product # 
Proteasome Fraction II Hela S3 cell extract human F3926
Proteasome Fraction II from rabbit F4051
26S Proteasome Fraction from rabbit P3863
20S Proteasome from rabbit P3988
 

Specificity:

 

Large protease complex directed by ubiqutination

 

Synthethic Substrates:

 

Z-Leu-Leu-Glu-7-amido-4-methylc, C0483
Z-Leu-Leu-Leu-7-amido-4-methylcoumarin, C0608
N-Succinyl-Leu-Leu-Val-Tyr-7-amido-4-trifluoromethylcoumarin, S4939

 

Inhibitors:

 

Cbz-Leu-Leu-Phe-al, C0358
Cbz-Leu-Leu-Leu-B(OH)2, C9857
Lactacystin, L6785
clasto-Lactacystin beta-lactone, L7035

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Proteinase from Aspergillus oryzae (Protease M)

Product Name
Product # 
Proteinase from Aspergillus oryzae P4032

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Proteinase 3

Product Name
Product # 
Proteinase 3 human, minimum 95% (PAGE), PR3 Ag P0615

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Proteinase A, E.C. 3.4.23.6

Product Name
Product # 
Proteinase A from baker's yeast (S. cerevisiae) P8892

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Proteinase K, E.C. 3.4.21.64

Product Name
Product # 
Proteinase K Tritirachium album, Lyophilized powder P2308
Proteinase K Tritirachium album, Buffered aqueous glycerol solution P4850
Proteinase K Tritirachium album, Lyophilized powder 1-3 unit/mg solid P8044
Proteinase K Tritirachium album, Lyophilized powder minimum 30 units/mg protein (biuret) P5056
Proteinase K Tritirachium album, minimum 500 units/mL Buffered aqueous glycerol solution P5568
Proteinase K Tritirachium album, Lyophilized powder minimum 30 units/mg protein P6556
 

Specificity:

 

Hydrolysis of keratin, and of other proteins with subtilisin-like specificity. Hydrolyses peptide amides

 

Inhibitors:

 

Diisopropyl fluorophosphate, D0879
Phenylmethylsulfonyl fluoride, P7626

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Protein C, E.C. 3.4.21.69

Product Name
Product # 
Protein C human P2562
Protein C from human plasma P2200
 

Specificity:

 

Cleaves factors Va and VIIIa

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Pyroglutamate aminopeptidase, E.C. 3.4.19.3

Product Name
Product # 
Pyroglutamate Aminopeptidase Pyrococcus furiosus P6236
 

Specificity:

 

Release of an N-terminal pyroglutamyl group from a polypeptide, the second amino acid generally not being Pro

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Renin, E.C. 3.4.23.15

Inquire on products

 

Specificity:

 

Cleavage of Leu-bond in angiotensinogen to generate angiotensin I

 

Inhibitors:

 

Pepstatin A, P5318
D-His-Pro-Phe-His-Leu-psi-(CH2NH)-Leu- Val-Tyr, H6137

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Rennin, E.C. 3.4.23.4

Product Name
Product # 
Rennin calf stomach R4877
 

Specificity:

 

Broad specificity similar to that of pepsin A. Clots milk by cleavage of a single Ser-Phe105-|-Met-Ala bond in k-chain of casein

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Streptokinase, E.C. 3.4.99.0

Product Name
Product # 
Streptokinase beta;-hemolytic Streptococcus (Lancefield Group C), Buffered aqueous solution approx. 100,000 units/mg protein S8026
Streptokinase beta;-hemolytic Streptococcus (Lancefield Group C), Lyophilized powder minimum 3,500 units/mg solid S3134

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Subtilisin, E.C. 3.4.21.62

Product Name
Product # 
Subtilisin A P5380
 

Specificity:

 

Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyses peptide amides

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Thermolysin, E.C. 3.4.24.27

Product Name
Product # 
Thermolysin Bacillus thermoproteolyticus rokko T7902
 

Specificity:

 

Preferential cleavage: Xaa-Leu > Xaa-Phe

 

Inhibitors:

 

Diethylenetriaminepentaacetic acid Free Acid, D6518
EDTA, ED2SS
EGTA, E4378
a2-Macroglobulin, M6159
1,10-Phenanthroline Monohydrate, P9375
Phosphoramidon, R7385

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Thrombin, E.C. 3.4.21.5

Product Name
Product # 
Thrombin bovine plasma, Lyophilized powder minimum 700 NIH units/mg protein (Bradford) T9681
Thrombin from human plasma, suitable (For routine use in thrombine time test) Lyophilized powder T9010
Thrombin from human plasma, cell culture, tested Lyophilized powder approx. 1,000 NIH units/mg protein (E1%18.3 at 280 nm) T4393
Thrombin bovine plasma, powder cell culture, tested T9549
Thrombin from rat plasma, Lyophilized powder minimum 1,000 units/mg protein (biuret) T5772
Thrombin from murine plasma, Lyophilized powder approx. 1,000 NIH units/mg protein (biuret) T8397
Thrombin from human plasma, Lyophilized powder 50-300 NIH units/mg protein T9677
Thrombin from human plasma, Lyophilized powder 50-300 NIH units/mg protein T0553
Thrombin from human plasma, suitable (For routine use in the thrombin time test) Lyophilized powder T8885
Thrombin from human plasma, Lyophilized powder T1063
Thrombin from human plasma, Lyophilized powder minimum 2,000 NIH units/mg protein (E1%18.3 at 280 nm) T6884
Thrombin from human plasma, suitable (For routine use in the thrombin time test.) Lyophilized powder T9135
Thrombin from human plasma, Lyophilized powder approx. 1,000 NIH units/mg protein (E1%18.3 at 280 nm) T7009
Thrombin bovine plasma, Lyophilized powder 600-1,500 NIH units/mg protein (biuret) T6634
Thrombin bovine plasma, Lyophilized powder 175-350 NIH units/mg protein (biuret) T4265
Thrombin bovine plasma, minimum 125 NIH units/mg protein (biuret) T9000
Thrombin bovine plasma, Lyophilized powder 50-175 NIH units/mg protein (biuret) T4648
Thrombin bovine plasma, Lyophilized powder 50-150 NIH units/mg protein T3399
Thrombin bovine plasma, Lyophilized powder T7513
 

Specificity:

 

Selective cleavage of Arg-Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B

 

Synthethic Substrates:

 

N-Benzoyl-Phe-Val-Arg 4-methoxy-b-naphthylamide hydrochloride, B1260
Boc-b-benzyl-Asp-Pro-Arg-7-amido-4-methylcoumarin hydrochloride, B4028
N-Benzoyl-Phe-Val-Arg-p-nitroanilide hydrochloride, B7632
Boc-Val-Pro-Arg-7-amido-4-methylcoumarin hydrochloride, B9385
4-Methylumbelliferyl 4-guanidinobenzoate hydrochloride monohydrate, M4149
Sar-Pro-Arg p-nitroanilide dihydrochloride, S9009
N-p-Tosyl-Gly-Pro-Arg 7-amido-4-methylcoumarin hydrochloride, T0273
N-(p-Tosyl)-Gly-Pro-Arg p-nitroanilide acetate salt, T1637
Thrombin generation chromogenic substrate, T3068

 

Inhibitors:

 

N-Acetyl-eglin C, E7888
4-(2-Aminoethyl) benzenesulfonyl fluoride hydrochloride, A8456
Antipain, A6191
a2-Antiplasmin, A0914
Antithrombin III, Bovine, A9141
Antithrombin III, Human, A2221
Antithrombin III, Rat, A6397
3,4-Dichloroisocoumarin, D7910
Diisopropyl fluorophosphate, D0879
Gabexate mesylate, G2417
Hirudin, H0393
Hirudin, H7016
Hirudin, H7380
Hirudin, H9022
Leupeptin, L2023
a2-Macroglobulin, M6159
N a-(2-Naphthalenesulfonylglycyl)-4-amidino-DL-phenylalaninepiperidide, N8514
Phenylmethylsulfonyl fluoride, P7626
N a-p-Tosyl-L-lysine chloromethyl ketone hydrochloride, T7254
D-Val-Leu-Lys-Chloromethyl ketone, V3763

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Tissue Plasminogen Activator

Product Name
Product # 
Tissue plasminogen activator from human melanoma cells T7776
 

Specificity:

 

Specific cleavage of Arg-Val bond in plasminogen to form plasmin

 

Synthethic Substrates:

 

Tissue Plasminogen Activator Substrate, T2943

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Trypsin, E.C. 3.4.21.4

Product Name
Product # 
Trypsin from bovine pancreas, suitable for protein sequencing Lyophilized powder T8658
Trypsin Gadus morhua (Atlantic cod), Type XX-S 5,000-10,000 units/mg protein Lyophilized powder T9906
Trypsin from bovine pancreas, Essentially salt-free, lyophilized powder 10,000-15,000 BAEE units/mg protein T8802
Trypsin from bovine pancreas, Essentially salt-free, lyophilized powder min. 10,000 BAEE units/mg protein T1426
Trypsin from bovine pancreas, Type XI Lyophilized powder min. 6,000 BAEE units/mg protein T1005
Trypsin porcine pancreas, Type IX-S Lyophilized powder 13,000-20,000 BAEE units/mg protein T0303
Trypsin from human pancreas, Salt-free, lyophilized powder T6424
Trypsin Acetylated from bovine pancreas, synthetic Type V-S approx. 10,000 BAEE units/mg protein (biuret) T6763
Trypsin from bovine pancreas, suitable for protein sequencing Lyophilized powder T8658
Trypsin from bovine pancreas, Lyophilized powder min. 7,500 BAEE units/mg solid T4665
Trypsin porcine pancreas, tablet 1 mg tablet Type II T7168
Trypsin from bovine pancreas, Type I approx. 10,000 BAEE units/mg protein T8003
Trypsin porcine pancreas, Type II-S Lyophilized powder 1,000-2,000 BAEE units/mg solid T7409
Trypsin, Dimethylated porcine, Proteomics Grade T6567
TrypZean™, recombinant, expressed in Corn Lyophilized powder minimum 3650 units/mg solid (USP) T3568
 

Specificity:

 

Preferential cleavage: Arg-Xaa , Lys-Xaa

 

Synthethic Substrates:

 

Na-Benzoyl-L-arginine-7-amido-4-methylcoumarin hydrochloride, B7260
Na-Benzoyl-DL-arginine-7-amido-4-methylcoumarin hydrochloride, B7385
BAEE, B4500
Na-Benzoyl-L-arginine 4-methoxy-b-naphthylamide hydrochloride, B0885
Na-Benzoyl-DL-arginineb-naphthylamide hydrochloride, B4750
Na-Benzoyl-L-arginine 4-nitroanilide hydrochloride, B3133
Na-Benzoyl-DL-arginine 4-nitroanilide hydrochloride, B4875

 

Inhibitors:

 

p-Aminobenzamidine dihydrochloride, A7148
4-(2-Aminoethyl) benzenesulfonyl fluoride hydrochloride, A8456
Antipain, A6191
a2-Antiplasmin, A0914
Antithrombin III, Human, A2221
Antithrombin III, Bovine, A9141
a1-Antitrypsin, A6150
a1-Antitrypsin, A9024
Aprotinin, A1153
Bdellin, B3906
Benzamidine Hydrochloride, B6506
Diisopropyl fluorophosphate, D0879
3,4-Dichloroisocoumarin, D7910
Ecotin, E1398
Gabexate mesylate, G2417
Leupeptin, L2023
a2-Macroglobulin, M6159
Phenylmethylsulfonyl fluoride, P7626
N a-p-Tosyl-L-lysine chloromethyl ketone hydrochloride, T7254
Trypsin Inhibitor, T0256
Trypsin Inhibitor, T2011
Trypsin Inhibitor, T4385
Trypsin Inhibitor, T9008
Trypsin Inhibitor, T9128
Trypsin Inhibitor, T9253
Trypsin Inhibitor, T9378
Trypsin-chymotrypsin inhibitor, T9777

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Tryptase, E.C. 3.4.21.59

Product Name
Product # 
Tryptase from human lung T7063
 

Specificity:

 

Preferential cleavage: Arg-Xaa, Lys-Xaa , but with more restricted specificity than trypsin

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Urokinase, E.C. 3.4.21.73

Product Name
Product # 
Urokinase from human urine U0633
 

Specificity:

 

Specific cleavage of Arg-Val bond in plasminogen to form plasmin

 

Inhibitors:

 

Glu-Gly-Arg-chloromethyl ketone, G2917

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