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Enzyme Explorer
Substrates Procedures
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| Substrate Product # |
Substrate |
Procedure |
| C0403 |
Casein fluorescein isothiocyanate from bovine milk, Type I |
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| C0528 |
Casein fluorescein isothiocyanate from bovine milk, Type III |
Fluorometric Protease Assay (PDF 75.3 KB) |
| C3777 |
Casein fluorescein isothiocyanate from bovine milk, Type II |
Fluorometric Protease Assay (PDF 75.3 KB) |
| C7078 |
Casein bovine milk, Technical Grade |
Colorimetric Protease Assay (PDF 17.7 KB), Instructional Video of Assay |
| C9801 |
Casein, Dimethylated |
Colorimetric Protease Assay: Cabacungan, J.C., et al., Anal. Biochem., 124, 272 (1982) |
| E0502 |
Elastin-Congo Red |
Colorimetric Protease Assay at 495 nm: Rust, L., et. al., Meth. Enzymol., 235, 557-559 (1994) and Shotton, D. M., Meth. Enzymol., XIX, 113-117 (1970) |
| E1500 |
Elastin-Orcein |
Colorimetric Protease Assay at 570 nm: Sachar, L. A., et. al., Proc. Soc. Exptl. Bio. Med., 90, 323 (1955) |
| G7765 |
Gelatin |
Use in Zymograms: Kleiner, D.E, and Stetler-Stevenson, W.G., Anal. Biochem., 218, 325-329 (1994) and Heussen, C. and Dowdle, E.B., Anal. Biochem., 102, 196-202 (1980) |
| H2625 |
Hemoglobin, Bovine |
Colorimetric Protease Assay (PDF 27.6 KB) |
| H6268 |
Remazol Brilliant Blue R |
Colorimetric Protease Assay at 595 nm: Rinderknecht, H., et al., Clin. Chem. Acta, 21, 197 (1968) |
| H9891 |
Hemoglobin, N,N-Dimethylated |
Colorimetric Protease Assay: Cabacungan, J.C., et al., Anal. Biochem., 124, 272 (1982) |
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