Automation GenElute HP 96 Well Miniprep Kit

Simple, rapid, high-throughput purification of transfection quality plasmid DNA in less than 50 minutes

The GenElute HP 96 Well Plasmid Miniprep Kit has been developed for use as a high-throughput method for the purification of plasmid DNA from recombinant E. coli cultures. This rapid and cost-effective system allows for the purification of up to 10 mg of high copy plasmid DNA per well. Purified DNA is of high quality and can be used in a variety of molecular biology applications including transfection, cloning, restriction digestion, in-vitro transcription and translation, and sequencing. Designed to complement Sigma’s MISSION® shRNA product line, the GenElute HP 96-Well Plasmid Miniprep Kit is optimized for the most demanding downstream transfection protocols. The result is purified plasmid DNA that can generate best-in-class transfection grade plasmid that delivers the highest viral titers of any 96-well plasmid kit on the market. The GenElute HP 96 Well Plasmid Miniprep Kit is compatible with liquid handling instrumentation. An automated method has been developed for the Biomek® FX from Beckman Coulter. The protocol is simple and rapid, resulting in a processing time of less than 50 minutes.

Automation Resources The following automation resources are available for download: Automated Method for use with the Biomek FX Workstation (Beckman Coulter) Automated Protocol for use with the Biomek FX Workstation (Beckman Coulter)

Applications Purified plasmid DNA is suitable for a variety of applications including: Transfection Cloning In vitro transcription and translation Restriction enzyme digestion Sequencing

Features & Benefits
Transfection-Grade Quality	Best-in-class production of viral titers from purified MISSION shRNA plasmid vectors.
High Yields	Up to 10 µg of high copy plasmid DNA per well.
Rapid Procedure	96 samples can be processed in less than 50 minutes.
Versatile	Generates purified plasmid DNA for a wide variety of downstream applications.

GenElute HP 96 Plasmid Miniprep Kits
Product #	Product Name	Package Size
NA9604	GenElute HP 96 Plasmid Miniprep Kits	4 x 96

GenElute is a trademark of Sigma-Aldrich, Inc. MISSION is a trademark of Sigma-Aldrich, Inc. Biomek FX is a registered trademark of Beckman Coulter, Inc.

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