Competent Cells

High Efficiency Competent Cells for your Sensitive Cloning Applications!

Sigma-Aldrich offers a wide range of chemically competent cells as well as Thunderbolt™ GC10™ electrocompetent cells. Our competent cells are offered at the highest transformation efficiencies for sensitive cloning applications. In addition, we offer a variety of strains to meet your specific needs for protein expression and plasmid propagation.

Features and Benefits
  • Convenient Unipack formats for one time use
  • Strains comparable to DH5α™ and DH10B™
  • High efficiencies
  • T1 phage resistance
Competent Cell Compendium
Cloning Strains
Expression Strains
Competent Cell Selection Table

The Competent Cell Compendium: Tips and Tools for Successful Transformations" Now Available
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Other Resources:


Cloning Strains

Features and Benefits

  • F' episome for single strand rescue
  • Strains for cloning methylated DNA
  • Blue/white selection

 

Product # 
Product Name
Size
G3169
GC5™ Competent Cells, Uni-pack
10 x 50 µl
20 x 50 µl
G3044
GC5™ Competent Cells, Standard Aliquots
5 x 200 µl
G7419
GC5™ Competent Cells, 96-well
1 EA
4 EA
G2919
GC10™ Competent Cells, Uni-Pack
10 x 50 µl
20 x 50 µl
G2794
GC10™ Competent Cells, Standard Aliquots
5 x 200 µl
T7699
Thunderbolt™ GC10™ ElectroCompetent Cells
5 x 80 µl
5 x 100 µl
J3895
JM109 Competent Cells, Uni-pack
10 x 50 µl
H3788
HB101 Competent Cells, Uni-pack
10 x 50 µl

 


Expression Strains

Features and Benefits

  • T1 phage resistance
  • Strains for T7 systems
  • Ion and omp T protease deficient

 

Product # 
Product Name
Size
B2685
BL21-T1R Competent Cells, Uni-pack
10 x 50 µl
B2935
BL21 (DE3)-T1R Competent Cells, Uni-pack
10 x 50 µl
B3310
BL21 (DE3) pLysS-T1R Competent Cells, Uni-pack
10 x 50 µl
B3435
BL21 (DE3) pLysE-T1R Competent Cells, Uni-pack
10 x 50 µl

 


Competent Cell Strain Selection Table

 

Feature GC5™ GC10™ Thunderbolt™ GC10™ JM109 HB101 BL21 BL21 (DE3) BL21 (DE3) pLysS BL21 (DE3) pLysE
Comparable to DH5a™
X
 
 
 
 
 
 
 
 
Comparable to NovaBlue and XL1 Blue
 
 
 
X
 
 
 
 
 
Comparable to DH10B™
 
X
X
 
 
 
 
 
 
Strain Background
K12
K12
K12
K12
K12xB
B
B
B
B
Transformation Efficiency (cfu/ug pUC plasmid DNA)
109
109
1010
108
108
106
107
106
106
Cloning Strain
X
X
X
X
X
 
 
 
 
Expression Strain
 
 
 
 
 
X
X
X
X
Blue/White Selection (with appropriate construct)
X
X
X
X
 
 
 
 
 
T1 bacteriophage resistant
X
X
X
 
 
X
X
X
X
T7 promoter systems
 
 
 
 
 
 
X
X
X
F' episome for single strand rescue
 
 
 
X
 
 
 
 
 
Ideal for cloning methylated DNA
 
X
X
 
X
 
 
 
 
lacI q (higher level of lac repressor)
 
 
 
X
 
 
 
 
 

GC5™, GC10™, and Thunderbolt™ are trademarks of GeneChoice ®, Inc.
DH5α™ and DH10B™ are trademarks of Invitrogen Corp.

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