Cloning & Expression

Genotypes of Selected E. coli Strains


Genotypes of Selected E. coli Strains

BL21 E. coli B F ompT gal [E. coli B is naturally dcm and lon] hsdSB

BL21(DE3) E. coli B F ompT gal [E. coli B is naturally dcm and lon] hsdSB with DE3, a λ prophage carrying the T7 RNA polymerase gene and lacIQ

C600 F [e14– (McrF) or e14+ (McrF+)] thr-1 leuB6 thi-1 lacY1 glnV44 rfbD1 fhuA21

CJ236 F Δ(HindIII)::cat (Tra+ Pil+ CamR )/ ung-1 relA1 dut-1 thi-1 spoT1 mcrA

GC5 F´ endA1 hsdR17 (rKmK+) glnV44 thi-1 recA1 gyrA (Nalr) relA1 Δ(lacIZYA-argF)U169 (φ80dlacΔ(lacZ)M15 fhuA

GM48 F thr leu thi lacY galK galT ara fhuA tsx dam dcm glnV44

HB101 F Δ(gpt-proA)62 leuB6 glnV44 ara-14 galK2 lacY1 Δ(mcrC-mrr) rpsL20 (Strr) xyl-5 mtl-1 recA13

JM83 F ara Δ(lac-proAB) rpsL (Strr)[φ80 dlacΔ(lacZ)M15] thi

JM101 F´traD36 proA+B+ lacIq Δ(lacZ)M15/ Δ(lac-proAB) glnV thi

JM103 F´ traD36 lacIqΔ(lacZ)M15 proA+B+/endA1 glnV sbcBC thi-1 rpsL (Strr) Δ(lac-pro) (P1) (rK–mK+ rP1+ mP1+)

JM105 F´ traD36 lacIqΔ(lacZ)M15 proA+B+/thi rpsL (Strr) endA sbcB15 sbcC hsdR4 (rK–mK+) Δ(lac-proAB)

JM107 F´ traD36 lacIq Δ(lacZ)M15 proA+B+/e14(McrA) Δ(lac-proAB) thi gyrA96 (Nalr) endA1 hsdR17 (rK mK+) relA1 glnV44

JM109 F´traD36 proA+B+ lacIq Δ(lacZ)M15/ Δ(lac-proAB) glnV44 e14- gyrA96 recA1 relA1 endA1 thi hsdR17

JM110 F´ traD36 lacIqΔ(lacZ)M15 proA+B+IrpsL (Strr) thr leu thi lacY galK galT ara fhuA dam dcm glnV44 Δ(lac-proAB)

K802 F e14- (McrA-) lacY1 or Δ(lac)6 glnV44 galK2 galT22 rfbD1 metB1 mcrB1 hsdR2 (rKmK+)

LE392 F e14 (McrA–) hsdR514 (rKmK+) glnV44 supF58 lacY1 or Δ(lacIZY)6 galK2 galT22 metB1 trpR55

MC1061 F araD139 Δ(ara-leu)7696 galE15 galK16 Δ(lac)X74 rpsL (Strr) hsdR2 (rKmK+) mcrA mcrB1

MM294 F endA1 hsdR17 (rKmK+) glnV44 thi-1 relA1 rfbD1 spoT1

NM477 C600 Δ(hsdMS-mcrB)5 (rKmK+ McrBC)

NM522 F´proA+B+ lacIq Δ(lacZ)M15/ Δ(lac-proAB) glnV thi-1 Δ(hsdS-mcrB)5

NM554 MC1061 recA13

NM621 F hsdR (rKmK+) mcrA mcrB glnV44 recD1009

RR1 HB101 RecA+

χ1776 F fhuA53 dapD8 minA1 glnV44 Δ(gal-uvrB)40 minB2 rfb-2 gyrA25 (Nalr) thyA142 oms-2 metC65 oms-1 (tte-1) Δ(bioH-asd)29 cycB2 cycA1 hsdR2 (rK mK+) mcrB1

References

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