Cloning & Expression

S-Gal™/LB Agar Blends for Detecting Gene Insertions

S-Gal is a trademark of Sigma Chemical Co.
The evidence is clear:

S-Gal produces the clearest contrast between lac+ and lac- colonies.

S-Gal X-gal plates
S-Gal Plate                X-gal Plate
 

S-Gal™ a new chromogen to detect gene insertions:
  • Contains a patented chromogenic substrate,
    S-Gal (3,4-cyclohexenoesculetin-b-D-galacto-
    pyranoside).
  • Replaces X-gal as the preferred substrate for
    measurement of b-galactosidase transformed
    cells used in molecular biology applications.
  • Hydrolyzed by b-galactosidase to yield an
    intense black insoluble precipitate.
  • Heat and light stable, non-toxic.


S-Gal, a new chromogenic substrate
for gene insertion detection.

 

S-Gal/LB Agar Blend

      Product. No. C 4478 is a complete powder mixture useful for detecting prokaryotic cells which
have been transformed with vectors containing the lacZ gene. It contains S-Gal, a new chromogenic
substrate, and isopropyl b-D-thio-galactopyranoside, (IPTG).

      IPTG initiates the transcription of the lacZ gene by binding to the lac repressor product of the
Lac I gene and allowing the lac operator to turn on transcription. The product of the lacZ gene is b-galactosidase. b-Galactosidase transformed cells have traditionally been measured by the
hydrolysis of X-gal (5-bromo-4-chloro-3-indolyl-b-D-galactoside) to a blue precipitate.

      Now we have S-Gal!

 

FEATURES BENEFITS
Faster colony color development. Pick colonies for faster results.
Lac+ colonies turn an intense black color. Easier to distinguish between lac + and lac - colonies.

May improve accuracy of automated colony pickers.
Complete with LB Agar, S-Gal, IPTG No need to make up separate solutions of X-gal and IPTG, saving time and effort.
No need to wait for media to cool before adding X-gal and IPTG.
No need for toxic solvents, such as DMF.
Convenient packaging. Each pouch holds the required amount of S-Gal/LB Agar Blend powder to make up to 500 ml of media. Ease of use for the researcher. Just add pre-blended powder to 500 ml of water, autoclave, add antibiotic if desired, and pour plates.
Saves time and labor.
S-Gal is not heat labile. Can be autoclaved or microwaved.
Provides flexibility to user.
S-Gal is not light sensitive. No need to shield product or plates from light.
Consistent performance and reliable results.
Just add water to S-Gal/LB Agar Blend, autoclave and pour your plates. It's that easy!

S-Gal/LB Agar Blend Formulation:

 

Prod. No. Component Description Grams per 500 ml
T9410 Tryptone 5.0
Y1625 Yeast Extract 2.5
S9625 Sodium Chloride 5.0
A5306 Agar, Bacteriological 6.0
S9811 S-Gal 0.150
F5879 Ferric Ammonium Citrate 0.250
I6758 IPTG 0.015
C4478 Complete Product, S-Gal/LB Agar Blend 18.915
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S-Gal/Kanamycin/LB Agar Blend


Product. No. S 1813 is a complete powder mixture useful for detecting prokaryotic cells which
have been transformed with vectors containing the lacZ gene. It contains S-Gal, kanamycin,
and isopropyl b-D-thio-galactopyranoside, (IPTG).

 

FEATURES BENEFITS
Faster colony color development. Pick colonies for faster results.
Lac+ colonies turn an intense black color. Easier to distinguish between lac + and lac - colonies.
May improve accuracy of automated colony pickers.
Complete with LB Agar, S-Gal, Kanamycin, IPTG No need to make up separate solutions of X-gal, Kanamycin and IPTG, saving time and effort.
No need to wait for media to cool before adding X-gal and IPTG.
No need for toxic solvents, such as DMF.
Convenient packaging. Each pouch holds the required amount of S-Gal/Kanamycin/LB Agar Blend powder to make up to 500 ml of media. Ease of use for the researcher. Just add pre-blended powder to 500 ml of water, autoclave, and pour plates.
Saves time and labor.
S-Gal is not heat labile. Can be autoclaved or microwaved.
Provides flexibility to user.
S-Gal is not light sensitive. No need to shield product or plates from light.
Consistent performance and reliable results.
Just add water to S-Gal/Kanamycin/LB Agar Blend, autoclave and pour your plates.
It's that easy!
Prod. No. Component Description Grams per 500 ml
T9410 Tryptone 5.0
Y1625 Yeast Extract 2.5
S9625 Sodium Chloride 2.5
A5306 Agar, Bacteriological 6.0
S9811 S-Gal 0.125
F5879 Ferric Ammonium Citrate 0.250
I6758 IPTG 0.025
K0879 Kanamycin 0.0175
S1813 Complete Product, S-Gal/Kanamycin/LB Agar Blend 16.4175
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S-Gal/LB Agar Blend, without IPTG


      Product. No. S 9938 is a complete powder mixture useful for detecting prokaryotic cells which
have been transformed with vectors containing the lacZ gene. It contains S-Gal and LB Agar.

      This product does not contain IPTG and is designed for those applications where IPTG is not
required. It also allows the researcher to precisely control the amount of inducer in applications
where this is critical.

 

FEATURES BENEFITS
Faster colony color development. Pick colonies for faster results.
Lac+ colonies turn an intense black color. Easier to distinguish between lac + and lac - colonies.
May improve accuracy of automated colony pickers.
Complete with LB Agar and S-Gal No need to make up a separate solution of X-gal, saving time and effort.
No need to wait for media to cool before adding X-gal.
No need for toxic solvents, such as DMF.
Convenient packaging. Each pouch holds the required amount of S-Gal/LB Agar Blend powder to make up to 500 ml of media. Ease of use for the researcher. Just add pre-blended powder to 500 ml of water, autoclave, add antibiotic and/or inducer if desired, and pour plates.
Saves time and labor.
S-Gal is not heat labile. Can be autoclaved or microwaved.
Provides flexibility to user.
S-Gal is not light sensitive. No need to shield product or plates from light.
Consistent performance and reliable results.
Just add water to S-Gal/LB Agar Blend, without IPTG, autoclave and pour your plates.
It's that easy!
Prod. No. Component Description Grams per 500 ml
T9410 Tryptone 5.0
Y1625 Yeast Extract 2.5
S9625 Sodium Chloride 2.5
A5306 Agar, Bacteriological 6.0
S9811 S-Gal 0.15
F5879 Ferric Ammonium Citrate 0.25
S9938 Complete Product, S-Gal/LB Agar Blend, without IPTG 16.40
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S-Gal/LB Agar Blends and Companion Products

These products are packaged especially for convenience in molecular biology applications.


Prod. No. Description Packaging
C4478 S-Gal/LB Agar Blend complete with IPTG Pre-weighed blend of components to make 500 ml of complete agar medium.
S1813 S-Gal/Kanamycin/LB Agar Blend complete with IPTG Pre-weighed blend of components to make 500 ml of complete agar medium.
S9938 S-Gal/LB Agar Blend, without IPTG Pre-weighed blend of components to make 500 ml of complete agar medium.
S7313 S-Gal, sodium salt Water soluble to >50 mg/ml. Available in 100 mg, 1 gram and 5 gram packaging.
S9811 S-Gal Original S-Gal. Available in 100 mg, 1 gram and 5 gram packaging.
A2804 Ampicillin, sodium salt Pre-weighed to give typical working concentrations when added to 1 liter of agar preparation.
K0879 Kanamycin Sulfate Pre-weighed to give typical working concentrations when added to 1 liter of agar preparation.
T8032 Tetracycline Hydrochloride Pre-weighed to give typical working concentrations when added to 1 liter of agar preparation.

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