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Cloning & Expression
S-Gal™/LB Agar Blend Microwave Protocol
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Add one pouch S-Gal/LB Agar Blend (complete with IPTG) to 500 ml deionized water.
Note: Powdered blend is stored at room temperature.
Time: 1-2 min.
Thoroughly mix S-Gal/LB Agar Blend in deionized water and microwave on high setting, just to boiling.
Time: 4-5 min.
Mix by swirling medium.
Note: This may be sufficient to dissolve the agar component. If not, heat for additional 15- to 30-second intervals, with mixing, until agar is in solution. To avoid "super heating", do not microwave unattended for extended periods.
Time: 5-8 min.
Add antibiotic supplement post-microwaving, after agar has cooled to approx. 50°C
Note: A 50°C water bath will minimize equilibration time.
Time: 10-30 min., if required.
Pour plates.
Note: S-Gal is not light sensitive. No need to protect plates from light.
Time: 10-15 min.
Total estimated time: 25 min.
For best results, allow plates to dry at room temperature, with lids on, for 16 hours following plating. |
S-Gal is a trademark of Sigma Chemical Co.
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