Insect Transfection Regents

• ESCORT Transfection Reagent
• ESCORT IV Transfection Reagent

ESCORT Transfection Reagent (E 9770)

ESCORT has been demonstrated to be an ideal transfection reagent for the transfection of Sf9 insect cells. Transfection of Sf9 cells using ESCORT results in higher efficiencies, better cell viability and requires less reagent and DNA compared to other transfection reagents. In addition, ESCORT may be used to transfect a wide variety of cell lines. For general transfection of cell lines, 5 µl is required for a 35 mm culture dish while 15 µl is sufficient for a 60 mm dish.

Features & Benefits

• High efficiency transfection of Sf9 cells
• Low toxicity
• Economical
• Efficiently transfects a wide variety of cell lines

ESCORT IV Transfection Reagent (L 3287)

Optimal transfection of both Sf9 and T.ni. insect cells is achieved using ESCORT IV. Using ESCORT IV, higher levels of transfection and resulting expression were seen in these cell lines compared to competitor products while maintaining lower levels of toxicity. For general transfection of cell lines, peak activities were achieved using 4-6 µg of ESCORT IV per µg of DNA in 35 mm dishes.

Features & Benefits

• Reagent of choice for Sf9 and T.ni. insect cells
• Low toxicity
• Recommended for cultured cell lines
• Useful for a wide variety of cell types

Transfection of Sf9 insect cells with ESCORT and ESCORT IV

Sf9 cells in exponential growth phase were seeded at a density of 3 x 10⁶ in 60 mm dishes. After allowing the cells to attach for 1 hour, the serum-containing medium was removed. The cells were transfected in serum-free medium with 3 µg of plasmid DNA containing a constitutively expressed LacZ gene. 12 µl of lipid reagent was used to transfect 3 µg of plasmid DNA. After addition of the lipid:DNA mixture, the cells were incubated for 5 hours. Following incubation, the medium was replaced with serum containing medium. After a 40 hour incubation period, the cells were stained for the presence of β-galactosidase. Panel A shows staining of untransfected Sf9 cells. Panel B contains Sf9 cells transfected using ESCORT while panel C demonstrates high efficiency transfection of Sf9 cells using ESCORT IV.

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