|
DiamondBac™: The Baculovirus System that Shines!
DiamondBac Baculovirus DNA is a linearized modified baculoviral DNA derived from AcNMPV (Autographa californica nuclear multiple polyhedrosis virus) that allows rapid and convenient generation of recombinant baculovirus clones. DiamondBac has been engineered to ensure complete linearization of the parental viral genome and contains a lethal deletion of the essential ORF1629 sequences. Therefore, parental viral DNA background is eliminated and recombination efficiencies approach 100% upon co-transfection with an appropriate transfer vector. DiamondBac is compatible with most commercially available polyhedrin-based transfer vectors such as the pPolh baculovirus transfer vector line.
- Reduce Time and Effort in Plaque Assays - Nearly 100% recombination efficiency
- High Level Expression - Up to a 6-fold increase in overall protein production
- Enhanced Protein Folding - Codes for a protein chaperone that aids in folding and proper disulfide bond formation
- Increased Cell Viability - Deletion of the p10 gene in the viral DNA coupled with use of recommended ESCORT™ or ESCORT™ IV Transfection Reagents leads to optimal cell viability
- Economical - Achieve a greater number of transfections per µg of viral DNA
No Detectable Parental Background with DiamondBac Baculovirus DNA!
DiamondBac Outperforms Competitor
Figure 1. Sf 21 cells are shown are at day 5 after infection with P1 linear viral stocks. The DiamondBac sample shows no sign of infection compared to the untreated cells while the competitor sample shows classic signs of infection.
P1 Titer Comparison to Co-transfected Sample
|
|
Figure 2. Co-transfections were performed in 6-well plates with 6.6 x 105 Sf21 cells per well using ESCORT IV Transfection Reagent. 100 ng DiamondBac DNA was transfected alone in the first sample and 100 ng of DiamondBac DNA plus 1µg of transfer vector were co-transfected in the second sample. After amplification in T-25 cm2 flasks, P1 stocks were generated in T-75 cm2 flasks and plaque assays were carried out using a standard plaque assay protocol. |
| Product # |
Product Name |
Unit |
| D6192 |
DiamondBac™ Baculovirus DNA (Sufficient for 15-25 co-transfections) |
5ug |
View pPolh Baculovirus Transfer Vectors that are compatible with the DiamondBac system.
View additional details on top-performing ESCORT Transfection Reagents for insect expression systems.
back to top
|
