DNA & RNA Purification

Blood Genomic DNA Miniprep Kit


Description: The GenElute™ Blood Genomic DNA Kit provides a simple and convenient technique to isolate high-quality genomic DNA from fresh or aged (older than 24 hours) whole blood. This kit combines the advantages of a silica-based system with a microspin format, eliminating the need for expensive resins and hazardous organic compounds.

  • Fast – less than 40 minutes
  • Easy – spin column format
  • Cost effective – more preps for the money

 Procedure: Whole blood is incubated with the appropriate enzymes to ensure efficient cell lysis and DNA realease from the cells. The whole blood is then lysed in a chatotropic salt-containing solution. DNA is bound to the silica based membrane and the remaining lysate is removed by centrifugation. After washing, to remove contaminants that are associated with aged whole blood samples, the DNA is eluted with buffer into a collection tube.

Application: Purified DNA (Figure 1) is ready for downstream applications such as restriction endonuclease digestions (Figure 2), PCR (Figure 3), Southern Blots and Automated Sequencing reactions.


GenElute Blood Genomic Flow Chart

Beneficial Features
Flexible

Isolate high quality genomic DNA from fresh or aged blood.

Fast

Less than 40 minutes from whole blood to isolated genomic DNA.

Convenient

A prewash solution is provided to ensure the removal of all contaminants associated with older blood samples and an RNase solution is provided for added convenience.

Purity

Genomic DNA has an A260/A280 ratio between 1.6 and 1.9.

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Order Information
Product Code Name Purifications per kit Technical Manual .pdf Short
Protocol.pdf
NA2000 GenElute Blood Genomic DNA Kit 10 X X
NA2010 GenElute Blood Genomic DNA Kit 70 X X
NA2020 GenElute Blood Genomic DNA Kit 350 X X

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Sample Data

Whole Blood collected in 3 different anticoagulants was isolated with the GenElute Blood Genomic DNA Kit and Supplier Q to obtain genomic DNA.

Figure 1. Purified genomic DNA from whole blood collected in vacutainer tubes, each containing a different anticoagulant (EDTA, Heparin or Sodium Citrate). Samples were isolated with either the GenElute Blood Genomic DNA kit or Supplier Q following both protocols in detail. The genomic DNA (100 ng/lane) was analyzed on a 0.8% agarose gel to show overall comparability with the main kit supplier. The whole blood (200 µl per sample) used was obtained from a human donor. Marker (M) used was Lambda Hind III .

GenElute Blood Genomic Isolation

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Graph 1. Yields of genomic DNA compared with leading Supplier Q from 3 different anticoagulants. Graph represents data from (Figure 1).
GenElute Blood Genomic Graph

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Genomic DNA purified by GenElute Blood Genomic DNA kits is suitable for restriction enzyme digestions.

Figure 2. Restriction Enzymes, EcoR I and Hind III were used to digest genomic DNA isolated with GenElute Blood Genomic DNA Kit. Whole blood was collected in 3 different anticoagulants: EDTA, Heparin and Sodium Citrate. A 100 ng aliquot of genomic DNA from each anticoagulant was initially digested with EcoR I (5 units per 1 µl digested at 37°C for 1.5 hours, and Hind III (10 units per 1 µl digested at 37°C for 1.5 hours) followed by electrophoresis (50 ng/lane) on a 0.8% agarose gel. Ladder (M) used was Lambda Hind III .

GenElute Blood Restriction Enzyme Gel

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PCR amplification of a 388 bp product isolated from genomic DNA.

Figure 3. Whole blood collected in 3 different anticoagulants was purified using the GenElute Blood Genomic DNA Kit. A 5 µl aliquot of each eluate was used as template in a 20 µl PCR reaction for 35 cycles. A 5 µl aliquot of each PCR reaction was resolved on a 2% agarose gel. The PCR marker (M) (Product Code P9577) used ranged from 50 bp to 2 kb.

GenElute Blood Genomic PCR

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