Extract-N-Amp™ Tissue PCR Kit

Description: The Extract-N-Amp™ Tissue PCR Kits provide all the reagents necessary to rapidly extract DNA from a wide variety of cells and tissues and amplify targets of interest by PCR (Figure 1).

Novel – single-step extraction of genomic DNA to PCR
Fast – tissue to PCR in 15 minutes
Convenient – no long enzymatic digestions

The Extract-N-Amp Tissue PCR Kit offers a novel extraction system that eliminates the need for either long enzymatic digestions or homogenization. The product includes a specially formulated hot start PCR ReadyMix™ for amplification directly from the extract. Extract-N-Amp products are Sigma Advanced Technology certified.

Procedure: Genomic DNA is extracted from a tissue sample that has been incubated in Tissue Preparation Solution and Extraction Solution for 10 minutes at room termperature. The sample is heated to 95 °C for 3 minutes and then mixed with a third solution to neutralize inhibitory substances prior to PCR. An aliquot of the DNA extract is then added directly to the optimized PCR mix supplied.

Application: Perfect for genotyping.
Watch the video protocol: Rapid Genotyping of Mouse Tissue Using Sigma's Extract-N-Amp Tissue PCR Kit

Beneficial Features

Starting Material	Mouse tail (0.5 to 1.0 cm) tissue (2 to 10 mg) buccal (10 µL)
Speed	Extraction of genomic DNA for PCR in 15 minutes
Convenient	No long enzymatic digestions
Safe	No phenol/chloroform or other hazardous materials
Specific	Hot Start antibody for highly specific PCR amplification of genomic DNA

Pick the formulation that is right for you.

The Extract-N-Amp Tissue PCR Kits include a specially formulated hot start PCR ReadyMix for amplification directly from the extract.

The PCR ReadyMix comes in two formulations:

REDExtract-N-Amp PCR Ready Mix – contains an inert dye that acts as a tracking dye and allows for convenient loading of PCR reactions onto agarose gels for analysis.
Extract-N-Amp PCR Ready Mix – no dye contained.

Watch the full video protocol here
Video brought to you by JoVE.com

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Order Information

Extract-N-Amp Tissue PCR Kits

Product Code	Name	Number of Extractions	Number of Amplifications	Technical Manual .pdf
XNAT2	Extract-N-Amp Tissue PCR Kit	100	100	X
XNAT2R	Extract-N-Amp Tissue PCR Kit	1000	1000	X

REDExtract-N-Amp Tissue PCR Kits

Product Code	Name	Number of Extractions	Number of Amplifications	Technical Manual .pdf
XNATS	REDExtract-N-Amp Tissue PCR Kit (contains REDTaq)	10	10	X
XNAT	REDExtract-N-Amp Tissue PCR Kit (contains REDTaq)	100	100	X
XNATR	REDExtract-N-Amp Tissue PCR Kit (contains REDTaq)	1000	1000	X

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Kit Contents

Extract-N-Amp Kit

Extraction Solution
Tissue Preparation Solution
Neutralization Solution B
Extract-N-Amp PCR Reaction Mix

REDExtract-N-Amp Kit

Extraction Solution
Tissue Preparation Solution
Neutralization Solution B
REDExtract-N-Amp PCR Reaction Mix

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Sample Data

PCR analysis of genomic DNA extracted from various samples using Sigma's Extract-N-Amp Tissue PCR Kit.

Figure 1. The Extract-N-Amp Tissue PCR Kit was used to extract and amplify genomic DNA from various sources. The extracted DNA was then amplified using the specially formulated hot start PCR ReadyMix. The products generated are 1181 bp for the Interleukin 1 b gene in mouse and 1820 bp for the Carnitine palmitoyltransferase II in human. Markers are PCR Marker (Product Code P9577).

Tissue Graph

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Sequence determination for 1181 bp Interleukin-1b mouse-tail PCR product.

Figure 2. The PCR product was purified with the GenElute™ PCR Clean-Up kit. The DNA Extraction and PCR were performed using Sigma's Extract-N-Amp Tissue PCR Kit. The sequence was obtained using ABI Big Dye™ Terminator Chemistry and the same primers as for the original PCR. Reaction products were resolved on an ABI 310.

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Stability of DNA in mouse-tail extracts.

Figure 3. Mouse-tail samples were extracted according to the procedure in the Technical Bulletin for the Extract-N-Amp Tissue PCR Kit. Extracts were stored at 37 °C instead of the recommended 4 °C to test accelerated storage and stability. Samples were removed at various time intervals for testing. Stability was determined by monitoring yield for quantitative PCR using SYBR® Green on an ABI Prism® 7700 instrument. Robust signals were obtained even after storage for 10 weeks.

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