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DNA & RNA Purification
Extract-N-Amp Seed™ PCR Kits
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Description: The Extract-N-Amp Seed™ PCR Kits contain all the reagents necessary to rapidly extract genomic DNA from seeds and amplify targets of interest by PCR (Figure 1).
- Novel – single-step extraction of seed genomic DNA to PCR
- Fast – seed to PCR in 15 minutes
- Convenient – no organic extraction, column purification or precipitation of DNA
The Extract-N-Amp Seed PCR Kit offers a novel extraction system that eliminates the need for DNA purification, organic extraction, centrifugation, heating, filtration or alcohol precipitation. The product includes a specially formulated hot start PCR ReadyMix™ for amplification directly from the extract. Extract-N-Amp products are Sigma Advanced Technology certified.
Procedure: Genomic DNA is extracted from ground seed material by incubation in a mixture of Extraction Solution and Seed Preparation Solution at 55 °C for 10 minutes. After the extraction is stopped by incubation at 95 °C for 3 minutes, an equal volume of Neutralization Solution B is added and the extract is ready for PCR. An aliquot of the extract is then added directly to the optimized PCR mix supplied.
Application: Perfect for genotyping.
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Beneficial Features
| Starting Material |
1 seed
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| Speed |
Extraction of seed genomic DNA for PCR in less than 15 minutes
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| Versatile |
Extract and amplify target DNA sequences from a variety of plant species (Figure 1)
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| High Specificity |
Hot Start antibody for highly specific PCR amplification of genomic DNA
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| Safe |
No phenol/chloroform or other hazardous materials
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Pick the formulation that is right for you.
The Extract-N-Amp Tissue PCR Kits include a specially formulated hot start PCR ReadyMix for amplification directly from the extract.
The PCR ReadyMix comes in two formulations:
- REDExtract-N-Amp PCR Ready Mix – contains an inert dye that acts as a tracking dye and allows for convenient loading of PCR reactions onto agarose gels for analysis.
- Extract-N-Amp PCR Ready Mix – no dye contained.
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Order Information
Extract-N-Amp Seed PCR Kits
| Product Code |
Name |
Number of Extractions |
Number of Amplifications |
Technical Manual .pdf |
| XNAS2 |
Extract-N-Amp Seed PCR Kit |
100 |
100 |
X |
REDExtract-N-Amp Seed PCR Kits
| Product Code |
Name |
Number of Extractions |
Number of Amplifications |
Technical Manual .pdf |
| XNASS |
REDExtract-N-Amp Seed PCR Kit
(contains REDTaq) |
10 |
10 |
X |
| XNAS |
REDExtract-N-Amp Seed PCR Kit
(contains REDTaq) |
100 |
100 |
X |
| XNA |
REDExtract-N-Amp Seed PCR Kit
(contains REDTaq) |
1000 |
1000 |
X |
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Kit Contents
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Extract-N-Amp Kit
- Seed Preparation Solution
- Extraction Solution
- Neutralization Solution B
- Extract-N-Amp PCR Ready Mix
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REDExtract-N-Amp Kit
- Seed Preparation Solution
- Extraction Solution
- Neutralization Solution B
- REDExtract-N-Amp PCR Ready Mix
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Sample Data
Multiplex PCR analysis of Extract-N-Amp Seed PCR Kit extracts from various seed types.
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Figure 1. Genomic DNA was extracted from the seeds using the protocol as described in the Extract-N-Amp Seed Technical Bulletin. All extracts were then amplified using the specially formulated JumpStart™ PCR mix and PCR primers multiplexed for both a universal chloroplast gene (~400-700 bp) and the acetylcoenzyme A carboxylase gene specific to wheat (964 bp).
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Stability of Soybean Extracts at 4 °C.
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Figure 2. Soybean seeds were extracted according to the procedure in the Technical Bulletin for the Extract-N-Amp Seed PCR Kit. 4 µL aliquots were analyzed immediately by quantitative PCR with SYBR Green detection on an ABI Prism® 7700. DNA standards for quantitative PCR were purified DNA prepared from soybean seeds using the GenElute Plant Genomic DNA Kit (Product code G2N70) and stored as single use aliquots at -20 °C. The soybean seed extracts were stored at 4 °C (recommended storage). Quantitative PCR was repeated after 2.5 and 5 weeks and then 2 and 6 months for the 4 °C samples. These results show that the extracts will be stable for at least 6 months at the recommended storage temperature of 4 °C.
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REDTaq allows for convenient loading onto agarose gels.
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Figure 3. Samples are loaded directly on an agarose gel after PCR amplification with RedTaq DNA Polymerase, obviating the need or addition of loading buffers and/or tracking dyes. The RedTaq dye approximately co-migrates with a 125 bp duplex.
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