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DNA & RNA Purification
GenElute™ HP Plasmid Miniprep Kits
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Description: The GenElute™ HP Plasmid Miniprep Kits offer purification of high-quality plasmid DNA in less than 30 minutes. These kits provide the convenience of binding columns that can be used with a vacuum or spin purification format. In addition, no alcohol precipitation is required.
- Sophisticated – Sigma's GenElute HP technology ensures improved performance
- Fast – Complete multiple preps in less than 30 minutes
- High Yields – Up to 25 µg of high-quality plasmid DNA
- Economic – lower cost + higher yields = significant savings
Procedure: An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the plasmid DNA onto silica in the presence of high salts. Contaminants are removed by a vacuum or spin wash step. Finally, the bound plasmid DNA is eluted in water or Tris-EDTA buffer.
Application: High-quality DNA is ready for immediate use for the most demanding applications, including transfection, restriction digestion, ligation, automated sequencing and PCR.
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Resuspend overnight culture and lyse cells.
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Neutralize and clear lysate.
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Prepare binding column.
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Load cleared lysate onto binding column and wash.
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Elute plasmid DNA.
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Beneficial Features:
| Yield |
Up to 25 µg of high-copy plasmid DNA |
| Speed |
Isolate high-quality DNA in less than 30 minutes |
| Flexibility |
Offers the convenience of a vacuum or spin format |
| Economic |
Lower cost per prep plus higher yields per prep equals double savings |
Ordering Information
| Product Code |
Product Name |
Preparations per Kit |
Technical Manual |
| NA0150S |
GenElute™ HP Plasmid Miniprep Kit |
10 |
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| NA0150 |
GenElute™ HP Plasmid Miniprep Kit |
70 |
| NA0160 |
GenElute™ HP Plasmid Miniprep Kit |
350 |
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Sample Data
Superior yield of plasmid DNA in less time.
Click on image to enlarge
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Click on image to enlarge
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Figure 1 and Figure 2. Comparison of plasmid yield and time/prep for the different purification systems used for isolating plasmid pCMV1-Sport-βgal. The table on the left depicts the average yield per preparation obtained by the Sigma GenElute HP Plasmid Miniprep as opposed to kits from Supplier Q and Supplier P. The figure on the right depicts the average time per preparation of the GenElute HP Plasmid Miniprep as compared to kits from Supplier Q and Supplier P. All plasmid DNA was isolated following each manufacturers' recommended protocol.
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High-quality plasmid DNA for Automated Sequencing.
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Figure 3. Capillary DNA sequencing of plasmid DNA purified with the GenElute HP Plasmid Miniprep kit. pCMV-SPORT-βgal (7.9 kb) was prepared from 400 µl overnight culture of E. coli host strain DH5α with the GenElute HP Plasmid Miniprep Kit. A 5 µl sample of the preparation was submitted, with adjustment in DNA concentration, to SeqWright DNA Technology Service (Houston, TX) for sequencing witha T7 sequencing primer and ABI BigDye™ terminator v3.1 on an ABI Prism® 3730 DNA Analyzer. The Phred > 20 score was greater than 800 bases.
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Click on image to enlarge
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