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Description: The GenElute™ HP Plasmid Miniprep Kits offer purification of high-quality plasmid DNA in less than 30 minutes. These kits provide the convenience of binding columns that can be used with a vacuum or spin purification format. In addition, no alcohol precipitation is required.
- Sophisticated – Sigma's GenElute HP technology ensures improved performance
- Fast – Complete multiple preps in less than 30 minutes
- High Yields – Up to 25 µg of high-quality plasmid DNA
- Economic – lower cost + higher yields = significant savings
Procedure: An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the plasmid DNA onto silica in the presence of high salts. Contaminants are removed by a vacuum or spin wash step. Finally, the bound plasmid DNA is eluted in water or Tris-EDTA buffer.
Application: High-quality DNA is ready for immediate use for the most demanding applications, including transfection, restriction digestion, ligation, automated sequencing and PCR.
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Resuspend overnight culture and lyse cells.
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Neutralize and clear lysate.
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Prepare binding column.
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Load cleared lysate onto binding column and wash.
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Elute plasmid DNA.
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