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DNA & RNA Purification
Mammalian Genomic DNA Miniprep Kit
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For purification of genomic DNA from a variety of mammalian sources
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Description: The GenElute Mammalian Genomic DNA Purification Kit provides a simple and convenient way to isolate pure, high molecular weight DNA from a variety of mammalian sources (Figure 1 and Figure 2). These kits use a silica-based membrane, specially selected for genomic DNA purification, in a convenient spin column format.
Quick look: G1N10, G1N70, G1N350
Procedure: Mammalian cells and tissues are lysed with a chaotropic salt-containing buffer to ensure denaturation of macromolecules. DNA is bound to the spin column membrane and the remaining lysate is removed by centrifugation. A filtration column is used to remove cell debris. After washing to remove contaminants, the DNA is eluted with buffer into a collection tube. The purified DNA may be used in many applications such as sequencing, cloning, blotting, restriction digestion, ligation, and PCR.
Features and Benefits:
- Typical DNA yields of 25 µg from 2 x 106 cultured cells or 30 µg from 25 mg of tissue (Figure 4)
- Preparation time is only 20 minutes after lysis
- Purified genomic DNA has A260/A280 ratios between 1.6 and 1.9
- No need for mechanical homogenization
- 40% more purification preps offered than other leading supplier
Storage: Room Temperature
R: 20/21/22 S: 26-36
Application: Purified DNA may be used in many applications such as sequencing, cloning, blotting, restriction digestion (Figure 3), ligation and PCR (Figure 4).
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Save time with the
Genomic DNA Miniprep Kit!
Unlike most kits on the market,
the elution buffer in our Sigma kit does not have to be heated. |
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Sample Data
| Table 1. Yields produced using GenElute Mammalian Genomic DNA Purification Kits (with RNase and Proteinase K treatment). |
| Material |
Amount |
Typical Yield |
| Jurkat Cells (human) |
2 x 106 Cells |
5 - 10 µg |
| HEK293 Cells (human) |
2 x 106 Cells |
10 - 20 µg |
| HeLa Cells (human) |
2 x 106 Cells |
15 - 25 µg |
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Mouse Pancreas Tissue
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20 mg |
10 - 25 µg |
| Mouse Spleen Tissue |
10 mg |
10 - 25 µg |
| Mouse Thymus Tissue |
16 mg |
10 - 25 µg |
| Mouse Lung Tissue |
20 mg |
5 - 15 µg |
| Mouse Brain Tissue |
16 mg |
5 - 15 µg |
| Mouse Kidney Tissue |
20 mg |
10 - 25 µg |
| Mouse Liver Tissue |
25 mg |
10 - 30 µg |
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Genomic DNA purified from mammalian tissues.
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Figure 1. Genomic DNA purified from mammalian tissues. Purified genomic DNA, from the indicated mouse tissues, was isolated with the GenElute™ Mammalian Genomic DNA Miniprep Kit. Genomic DNA (200 ng/lane) was analyzed on a 0.8% agarose gel to illustrate yield and integrity. Markers are lambda DNA digested with Hind II. (Product No. D9780)
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Genomic DNA purified from cells using GenElute Mammalian Genomic DNA Purification Kit.
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Figure 2. Genomic DNA purified from mammalian cells. Purified genomic DNA was isolated with the GenElute Mammalian Genomic DNA Purification Kit from 2 x 106 cells of the sources indicated. The genomic DNA (200 ng/lane) was analyzed on a 0.8% agarose gel. Marker is a Lambda Hind III digest (0.5-23 kb).
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Genomic DNA purified with GenElute Mammalian Genomic DNA Purification Kit is suitable for restriction enzyme digestions.
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Figure 3. Genomic DNA (200 ng) was digested with EcoR I (10 units in 20 µl at 37°C for 4 hours) followed by electrophoresis (150 ng/lane) on a 0.8% agarose gel. The undigested DNA was incubated under the same conditions, but without EcoR I. Marker is a Lambda Hind III digest (0.5-23 kb).
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Yield Comparison.
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Figure 4. Yields of genomic DNA compared with leading Supplier Q using 2 x 200 µl elutions.
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Order Information
| Product Code |
Name |
Purifications per kit |
Technical Manual .pdf |
Short
Protocol.pdf |
| G1N10 |
GenElute Mammalian Genomic DNA Miniprep |
10 |
X |
X |
| G1N70 |
GenElute Mammalian Genomic DNA Miniprep |
70 |
X |
X |
| G1N350 |
GenElute Mammalian Genomic DNA Miniprep |
350 |
X |
X |
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