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Description: The GenElute Mammalian Genomic DNA Purification Kit provides a simple and convenient way to isolate pure, high molecular weight DNA from a variety of mammalian sources (Figure 1 and Figure 2). These kits use a silica-based membrane, specially selected for genomic DNA purification, in a convenient spin column format.
Quick look: G1N10, G1N70, G1N350
Procedure: Mammalian cells and tissues are lysed with a chaotropic salt-containing buffer to ensure denaturation of macromolecules. DNA is bound to the spin column membrane and the remaining lysate is removed by centrifugation. A filtration column is used to remove cell debris. After washing to remove contaminants, the DNA is eluted with buffer into a collection tube. The purified DNA may be used in many applications such as sequencing, cloning, blotting, restriction digestion, ligation, and PCR.
Features and Benefits:
- Typical DNA yields of 25 µg from 2 x 106 cultured cells or 30 µg from 25 mg of tissue (Figure 4)
- Preparation time is only 20 minutes after lysis
- Purified genomic DNA has A260/A280 ratios between 1.6 and 1.9
- No need for mechanical homogenization
- 40% more purification preps offered than other leading supplier
Storage: Room Temperature
R: 20/21/22 S: 26-36
Application: Purified DNA may be used in many applications such as sequencing, cloning, blotting, restriction digestion (Figure 3), ligation and PCR (Figure 4).
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Save time with the
Genomic DNA Miniprep Kit!
Unlike most kits on the market,
the elution buffer in our Sigma kit does not have to be heated. |
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