DNA & RNA Purification

GenElute™ Plasmid Miniprep Kit


Description: GenElute™ Plasmid Miniprep Kit offers a simple, rapid, and cost-effective method for isolating up to 20 µg plasmid DNA from E. coli cultures.

  • Fast – 24 preps in 30 minutes
  • Convenient – spin column format
  • Cost effective – more preps for the money

Procedure: Bacterial cells are harvested via centrifugation, subjected to a modified alkaline-SDS lysis procedure and the DNA adsorbed onto silica in the presence of high salts. Contaminants are then removed by a simple wash step. Bound DNA is eluted in water or Tris-EDTA buffer.

Application: Plasmid DNA is ready for immediate use in restriction enzyme digestion (Figure 1) cloning, PCR, transcription, conventional and automated sequencing (Figure 2).



GenElute Bacterial Genomic Flow Chart

Beneficial Features
Culture Volume

1 - 5 ml overnight bacterial culture

Yield

Up to 20 µg of plasmid DNA

Speed

30 minutes for 24 preps

Versatile

Contains additional wash buffer for use with EndA+ E. coli bacterial strains (e.g., HB101, JM101, BL21)

Specific

No detectable genomic DNA or RNA contamination

Safe

No phenol/chloroform required

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Order Information
Product Code Culture Volume (ml) Plasmid
Yield (µg)
Time
Required
Number
of Preps
Technical Manual .pdf Short protocols .pdf
PLN10
PLN70
PLN350
1 to 5 30 to 20 Hands On: < 10 min.
Total: < 30 min.
10
70
350
X
X
X
X
X
X
PLD35
PLD140
5 to 40 25 to 200 Hands On: < 10 min.
Total: < 40 min.
35
140
X
X
X
X
PLX15
PLX50
25 to 130 200 to
1,200
Hands On: < 10 min.
Total: < 40 min.
15
50
X
X
X
X

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Sample Data

Plasmid DNA purified with GenElute Plasmid Miniprep Kit is suitable for restriction enzyme digestions.

Figure 1. Restriction digestions of pUC19 purified from JM109 using the GenElute Plasmid Miniprep Kit. Lane 1 was an undigested plasmid, lanes 2-4 were cut with Bam HI, Eco RI and Pst I respectively. Lane M was 1 kb ladder (11 fragments from 0.5 to 10 kb). Electrophoresis was on a 1% agarose gel.
NAP Plasmid Gel

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High-quality plasmid DNA for Automated Sequencing.

Figure 2. Electropherogram revealing >600 bases of sequence from pUC-TMV using GenElute Plasmid Miniprep Kit. Cycle Sequencing was performed using 500 ng of template, a T7 sequencing primer, and ABI Big DyeTM terminator chemistry. Sequencing reactions were resolved on an ABI Prism 377 XL instrument with a 48 cm gel cassette containing 4.5% AutoPAGE Plus acrylamide at 2.4 kV, 48°C for 7 hours.

Click Image to View Sequence in its Entirety

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