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Description: GenElute™ Plasmid Miniprep Kit offers a simple, rapid, and cost-effective method for isolating up to 20 µg plasmid DNA from E. coli cultures.
- Fast – 24 preps in 30 minutes
- Convenient – spin column format
- Cost effective – more preps for the money
Procedure: Bacterial cells are harvested via centrifugation, subjected to a modified alkaline-SDS lysis procedure and the DNA adsorbed onto silica in the presence of high salts. Contaminants are then removed by a simple wash step. Bound DNA is eluted in water or Tris-EDTA buffer.
Application: Plasmid DNA is ready for immediate use in restriction enzyme digestion (Figure 1) cloning, PCR, transcription, conventional and automated sequencing (Figure 2).
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Beneficial Features
| Culture Volume |
1 - 5 ml overnight bacterial culture
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| Yield |
Up to 20 µg of plasmid DNA
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| Speed |
30 minutes for 24 preps
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| Versatile |
Contains additional wash buffer for use with EndA+ E. coli bacterial strains (e.g., HB101, JM101, BL21)
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| Specific |
No detectable genomic DNA or RNA contamination
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| Safe |
No phenol/chloroform required
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Order Information
| Product Code |
Culture Volume (ml) |
Plasmid
Yield (µg) |
Time
Required |
Number
of Preps |
Technical Manual .pdf |
Short protocols .pdf |
PLN10
PLN70
PLN350
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1 to 5 |
30 to 20 |
Hands On: < 10 min.
Total: < 30 min. |
10
70
350 |
X
X
X
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X
X
X
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PLD35
PLD140
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5 to 40 |
25 to 200 |
Hands On: < 10 min.
Total: < 40 min. |
35
140 |
X
X
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X
X
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PLX15
PLX50
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25 to 130 |
200 to
1,200 |
Hands On: < 10 min.
Total: < 40 min. |
15
50 |
X
X
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X
X
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Sample Data
Plasmid DNA purified with GenElute Plasmid Miniprep Kit is suitable for restriction enzyme digestions.
| Figure 1. Restriction digestions of pUC19 purified from JM109 using the GenElute Plasmid Miniprep Kit. Lane 1 was an undigested plasmid, lanes 2-4 were cut with Bam HI, Eco RI and Pst I respectively. Lane M was 1 kb ladder (11 fragments from 0.5 to 10 kb). Electrophoresis was on a 1% agarose gel. |
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High-quality plasmid DNA for Automated Sequencing.
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Figure 2. Electropherogram revealing >600 bases of sequence from pUC-TMV using GenElute Plasmid Miniprep Kit. Cycle Sequencing was performed using 500 ng of template, a T7 sequencing primer, and ABI Big DyeTM terminator chemistry. Sequencing reactions were resolved on an ABI Prism 377 XL instrument with a 48 cm gel cassette containing 4.5% AutoPAGE Plus acrylamide at 2.4 kV, 48°C for 7 hours.
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