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DNA & RNA Purification
SigmaSpin™ Post-Reaction Clean-Up Plates and Columns
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SigmaSpin Post-Reaction Clean-Up Columns
SigmaSpin Sequencing Reaction Clean-Up Columns are ideal for lower throughput applications, such as clean up of probe labeling reactions or small numbers of sequencing reactions.
These columns can accept sample volumes up to 100 µl. Each column comes with a collection tube to collect the DNA during centrifugation. |
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SigmaSpin 96-Well Sequencing Reaction Clean-Up Plates
SigmaSpin 96-Well Sequencing Reaction Clean-Up plates provide a fast, simple, and highly efficient method for removing unincorporated dyes, excess salts, and other interfering reaction components. Each plate is packed with a size-exclusion resin, prehydrated with molecular biology grade water. Unique plate design incorporates drip directors to eliminate contamination between samples. SigmaSpin has been tested in high-throughput genome centers and core facilities with ABI Prism 3700, 3100, 377 and Amersham Biosciences MegaBACE automated DNA sequencers. SigmaSpin is compatible with all standard terminator chemistries including BigDye, DYEnamic ET and Thermo Sequenase II. |
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| SigmaSpin 96-Well Clean-Up Plate |
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Order Information
| Product Code |
Product Name |
Size |
| S5059 |
SigmaSpin Post-Reaction Clean-Up Columns |
70 each |
| S4309 |
SigmaSpin 96-Well Sequencing Reaction Clean-Up Plates |
2 each |
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Beneficial Features
| Versatile |
Compatible with all standard terminator chemistries and all commercially available DNA sequencers
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| Fast |
Quick and easy protocol requires 6 minutes total centrifugation
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| High Performance |
Pre-qualified size-exclusion resin guarantees optimum performance
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| Recovery |
Load and recover sample volumes down to 5 µl
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| Precision |
Unique drip directors prevent cross-contamination between samples
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Sample Data
SigmaSpin Removes Interfering Reaction Components and Eliminates Dye Blobs
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Figure 1. Sequencing reaction was purified using a SigmaSpin 96-well plate. pFLAG™ MAC plasmid (E 5644) was sequenced using BigDye™ Terminator v 3.0 chemistry (20 µl total reaction volume; BigDye premix diluted 1:1 with SeqSaver™ (S3938)). Data was generated on an ABI Prism. 3700 DNA Analyzer with POP-6™ polymer and 10X CE Buffer (B4930). Phred 20 > 600.
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