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We provide GenElute™ PCR clean-up, agarose spin columns and minus EtBr spin columns for purification of single or double stranded PCR amplification fragments; as well as extraction of DNA fragments from Ethidium Bromide (EtBr) stained agarose gels.
Applications for these kits would include enzymatic reactions, automated sequencing, cloning, microarray analysis, ligation, restriction enzyme digestions, and PCR.
| Product |
Yield |
Speed |
Starting Material |
Product Description
|
Technology |
Applications |
GenElute™
PCR Clean-Up Kit |
Up to 95% recovery |
< 10 minutes |
100 µl or 10 µg of DNA |
Purification of single or double stranded PCR
(100 bp to
10 kb) amplification products |
Silica membrane with a spin column format |
Enzymatic Reactions, automated sequencing, Cloning and Microarray Analysis |
| GenElute™ Agarose Spin Columns |
Up to 80% recovery |
10 minutes |
< 200 mg gel slice |
Purification of DNA fragments (50 bp to
10 kb) from agarose gel slices |
Membrane filters with a spin column format |
Ligation, restriction enzyme digestion, cloning and PCR |
| GenElute™ Minus EtBr Spin Columns |
Up to 70% recovery |
10 minutes |
< 200 mg gel slice |
Extraction of DNA fragments (50 bp to
10 kb) from Ethidium Bromide (EtBr) stained agarose gels or EtBr-containing solutions |
Membrane filters with a spin column format |
Ligation, restriction enzyme digestion, cloning and PCR |
| GenElute™ Gel Extraction Kit |
Up to 80% recovery |
N/A |
Up to 3.5 g gel slice |
Purification of linear and plasmid DNA fragments from standard or low-melting agarose gels |
Silica membrane with a spin column format |
Automated DNA Sequencing, PCR, Restriction Digestion, Cloning, and Labeling |
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