DNA & RNA Purification

TRI Reagent® RNA Isolation Reagent

Description

TRI Reagent is an improved version of the single-step total RNA isolation reagent developed by Chomczynski1. The RNA isolation method based on this reagent is widely recognized and proven for RNA applications and is supported by a substantial publication list.2,3,4,5 It is ideal for quick, economical, and efficient isolation of total RNA or the simultaneous isolation of RNA, DNA and proteins from samples of human, animal, plant, yeast, bacterial and viral origin.

Beneficial Features

Yield Better yields than traditional guanidine thiocyanate or cesium chloride methods
Versatile Works with many sources including human, plant, yeast, bacterial or viral
Convenience Three convenient formulations
Efficient Easily scalable RNA isolation

TRI Reagent RNA Isolation Reagent

Product Number Product Description Product Size
T9424 TRI Reagent RNA, DNA and Protein Isolation Reagent 25, 100 and 200 ml
T3809 TRI Reagent BD 25, 100 and 200 ml
T3934 TRI Reagent LS 25, 100 and 200 ml
B9673 1-Bromo-3-Chloropropane (BCP) 200 ml

TRI Reagent Formulations

Product Name Sample Type Sample Volume TRI Reagent Volume
TRI Reagent Tissues, cultured adherent cells, cell pellets up to 100 mg tissue, 107 cells or 102 cm plate area 1 ml
TRI Reagent BD Whole blood, plasma, serum 0.25 ml blood derivatives 0.75 ml
TRI Reagent LS Cell suspension, CSF, amniotic fluid 0.25 ml fluid samples 0.75 ml

Typical RNA Yield

Tissue Yield
(µg RNA/mg tissue)
Cultured Cells Total RNA Yield
(µg RNA/mg tissue)
Liver 6 - 10 Epithelial 8 - 15
Spleen 6 - 10 Fibroblast 5 - 7
Kidney 3 - 4    
Skeletal Muscle 1 - 1.5    
Brain 1 - 1.5    
Placenta 1 - 4    

Total RNA was prepared from HeLa cells using TRI Reagent from Sigma and other various suppliers

TRI Reagent® RNA Isolation Reagent   Figure 1. Total RNA from HeLa cells was prepared using TRI Reagents from Supplier R (Lane 1), Sigma (Lane 2) and Supplier L (Lane 3). A 2 µl aliquot out of 200 µl total RNA was analyzed on a 1% agarose gel. RNA Marker (M) used ranged from 0.2 bp - 10 kb (Product No. R7020).

References

  1. Chomczynski, P. and Sacchi, N., Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162: 156 (1987).
  2. Chomczynski, P., A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. BioTechniques 15: 532-537 (1993).
  3. Mackey, K. and Chomczynski, P., Long-term stability of RNA isolation reagents. J. NIH Res. 8: 72 (1996).
  4. Wilfinger, W., et al., Effect of pH and ionic strength on the spectrophotometric assessment of nucleic acid purity.BioTechniques 22: 474-481(1997).
  5. Chomczynski, P. and Mackey, K., Modification of the TRI Reagent. procedure for isolation of RNA from polysaccharide- and proteoglycan-rich sources.BioTechniques 19: 924-945(1995).

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