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DNA & RNA Purification
TRI Reagent® RNA Isolation Reagent | ||
Description
TRI Reagent is an improved version of the single-step total RNA isolation reagent developed by Chomczynski1. The RNA isolation method based on this reagent is widely recognized and proven for RNA applications and is supported by a substantial publication list.2,3,4,5 It is ideal for quick, economical, and efficient isolation of total RNA or the simultaneous isolation of RNA, DNA and proteins from samples of human, animal, plant, yeast, bacterial and viral origin.
Beneficial Features
| Yield | Better yields than traditional guanidine thiocyanate or cesium chloride methods |
| Versatile | Works with many sources including human, plant, yeast, bacterial or viral |
| Convenience | Three convenient formulations |
| Efficient | Easily scalable RNA isolation |
TRI Reagent RNA Isolation Reagent
| Product Number | Product Description | Product Size |
| T9424 | TRI Reagent RNA, DNA and Protein Isolation Reagent | 25, 100 and 200 ml |
| T3809 | TRI Reagent BD | 25, 100 and 200 ml |
| T3934 | TRI Reagent LS | 25, 100 and 200 ml |
| B9673 | 1-Bromo-3-Chloropropane (BCP) | 200 ml |
TRI Reagent Formulations
| Product Name | Sample Type | Sample Volume | TRI Reagent Volume |
| TRI Reagent | Tissues, cultured adherent cells, cell pellets | up to 100 mg tissue, 107 cells or 102 cm plate area | 1 ml |
| TRI Reagent BD | Whole blood, plasma, serum | 0.25 ml blood derivatives | 0.75 ml |
| TRI Reagent LS | Cell suspension, CSF, amniotic fluid | 0.25 ml fluid samples | 0.75 ml |
Typical RNA Yield
| Tissue | Yield (µg RNA/mg tissue) |
Cultured Cells | Total RNA Yield (µg RNA/mg tissue) |
| Liver | 6 - 10 | Epithelial | 8 - 15 |
| Spleen | 6 - 10 | Fibroblast | 5 - 7 |
| Kidney | 3 - 4 | ||
| Skeletal Muscle | 1 - 1.5 | ||
| Brain | 1 - 1.5 | ||
| Placenta | 1 - 4 |
Total RNA was prepared from HeLa cells using TRI Reagent from Sigma and other various suppliers
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Figure 1. Total RNA from HeLa cells was prepared using TRI Reagents from Supplier R (Lane 1), Sigma (Lane 2) and Supplier L (Lane 3). A 2 µl aliquot out of 200 µl total RNA was analyzed on a 1% agarose gel. RNA Marker (M) used ranged from 0.2 bp - 10 kb (Product No. R7020). |
References
- Chomczynski, P. and Sacchi, N., Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162: 156 (1987).
- Chomczynski, P., A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. BioTechniques 15: 532-537 (1993).
- Mackey, K. and Chomczynski, P., Long-term stability of RNA isolation reagents. J. NIH Res. 8: 72 (1996).
- Wilfinger, W., et al., Effect of pH and ionic strength on the spectrophotometric assessment of nucleic acid purity.BioTechniques 22: 474-481(1997).
- Chomczynski, P. and Mackey, K., Modification of the TRI Reagent. procedure for isolation of RNA from polysaccharide- and proteoglycan-rich sources.BioTechniques 19: 924-945(1995).