DNA & RNA Purification

RNAlater™ Storage Solution

Description: RNAlater™ is an aqueous, non-toxic tissue and cell storage reagent that stabilizes and protects cellular RNA in intact, unfrozen tissue and cell samples. RNAlater eliminates the need to immediately process samples or to freeze samples in liquid nitrogen for later processing. RNAlater can be used with various downstream applications including mRNA and total RNA isolation, histology and immunocytochemistry and is compatible with Sigma's GenElute isolation kits.

RNAlater is easy to use. Simply cut tissue samples to be stored so they are less than 0.5 cm in at least one dimension and submerge in 5 volumes of RNAlater. Small organs, such as rat kidney, liver or spleen can be stored in whole in RNAlater. When ready to isolate the RNA, remove the tissue from RNAlater and process as though just harvested. For cell storage, resuspend pelleted cells in a small amount of PBS before adding 5-10 volumes of RNAlater. Before preparing RNA, pellet cells and discard supernatant.

RNA later Flow Chart

Features and Benefits

Fast
Rapidly permeates tissues to stabilize and protect cellular RNA with immediate RNAse inactivation.
Stable
Stabilizes samples at room temperature for up to one week or indefinitely at -20 °C for archiving needs
Highly Efficient
No compromise in RNA quality following mRNA or total RNA isolation
Safe
Aqueous non-toxic solution allows downstream tissue processing

RNAlater Storage Solution

Product Code
Name
Size
RNAlater Tissue Storage and
RNA Stabilization Solution
100 ml
500 ml

Application data for RNAlater

Agarose gel image

Figure 1. Stabilizes Samples at Room Temperature For Up To One Week. Quality of RNA isolated from tissue stored in RNAlater Solution. Fresh mouse tissues were dissected and stored in RNAlater at 37 °C for 1 day, room temperature for 1 week or 4 °C for 1 month. RNA was isolated using TRI® Reagent (MRC) and analyzed using denaturing agarose gel electrophoresis.

Agagose gel image


Figure 2. Superior Protection Against RNAse Degradation. A 5 ml aliquot of RNAse A (Product Code R6513; serially diluted to final concentrations of 4.5x10-5-4.5x10-11 units/µl) was added to 5 µg RNA (Product Code R7020) in 15 µl containing either 10µl of RNAlater (top panel) or TE buffer (bottom panel). Reactions were incubated at 37 °C for 20 minutes, purified using the GenEluteTM Total RNA kit (Product Code RTN10) and analyzed on a 1% agarose gel.

Licensed for distribution by Ambion, Inc., The RNA Company. RNAlaterTM is a trademark of Ambion, Inc., Austin, Texas and is protected by various U.S. and foreign patents.

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