Description: The SYBR Green Extract-N-Amp Tissue PCR Kit contains all the reagents needed for rapid extraction, amplification and detection of genomic DNA from mouse tails and other animal tissues, buccal swabs, hair shafts, and saliva.
- Novel – all liquid, single-step extraction of genomic DNA for quantitative PCR (qPCR)
- Fast – tissue to qPCR in 15 minutes
- Convenient – no long enzymatic digestions and no column purifications
The SYBR Green Extract-N-Amp Tissue PCR Kit offers an innovative extraction system that eliminates the need for either long enzymatic digestions or homogenization. The product includes a specially formulated Hot Start SYBR Green PCR ReadyMix™ for amplification and quantitation directly from the extract.
Procedure: DNA is rapidly extracted from a tissue by incubating the sample with a mixture of the Extraction Solution and the Tissue Preparation Solution at room temperature for 10 minutes. After a 3-minute heat denaturing step, an equal volume of Neutralization Solution B is added to the extract to neutralize inhibitory substances. The extract is ready for real-time PCR in any plate-based real-time thermal cycling system in less than
15 minutes!
Application: Ideal for genotyping, gene copy number experiments, and amplifying and quantifying DNA from multiple tissue sample types.
Watch the video protocol Rapid Genotyping of Mouse Tissue Using Sigma's Extract-N-Amp Tissue PCR Kit
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Incubate with Extraction Solution for 10 minutes at room temperature.
Heat at 95 °C for 3 minutes.
Add Neutralization Solution.
Mix aliquot with Extract-N-Amp™ SYBR® Green PCR ReadyMix™ and primers.
Transfer to thermal cycler.
PCR.
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