UltraClear™ Sequencing Reaction Clean-Up Kit

The Advantage is Clear : Sigma’s UltraClear™ Sequencing Reaction Clean-Up Kit

The UltraClear Sequencing Reaction Clean-Up Kit offers a rapid and simple method for the clean-up of DNA sequencing reactions in a 96-well format. The UltraClear plate uses ultrafiltration membranes to separate low molecular weight contaminants,such as unincorporated dye terminators, dNTPs, and residual salts from the sequencing reaction products.

UltraClear Protocol
	Dilute Sequencing Reactions Load Ultra Clear Plate Centrifuge Add 40 µl Sequencing Solution Resuspend the Purified Sequencing Products Transfer Sequencing Product to an Appropriate Injection Plate for each configuration.

Features and Benefits

Excellent quality – PHRED q>20 scores greater than 850 bases (See Data)
Scientifically engineered to use less BigDye® version 3.1 chemistry and save money
Optimized centrifugation procedures for maximum flexibility
Partial 96-well plate can be used and stored at room temperature

Order Information

Product Code	Product Name	Size
UC9601	UltraClear Sequencing Reaction Clean-Up	1 x 96
UC9604	UltraClear Sequencing Reaction Clean-Up	4 x 96

High Quality Sequence Data with UltraClear Kit

Excellent PHRED q>20 Scores Compared to Competition

Data quality analysis based on PHRED q>20 scores. Samples analyzed on the ABI Prism® 3700 under standard run conditions. M13 template was prepared using 1/8 BigDye Terminator v3.1.

Optimized Protocol Accommodates a Variety of Spin Forces and Times

Spin Force	Time	Avg. PHRED 20	SD
1,000 x g	30 minutes	887	11

1,500 x g	20 minutes	870	15

2,500 x g	15 minutes	880	16

3,500 x g	10 minutes	859	39

Average PHRED q >20 scores for each spin force/time configuration tested. N=8

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