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The Advantage is Clear : Sigma’s UltraClear™ Sequencing Reaction Clean-Up Kit
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The UltraClear Sequencing Reaction Clean-Up Kit offers a rapid and simple method for the clean-up of DNA sequencing reactions in a 96-well format. The UltraClear plate uses ultrafiltration membranes to separate low molecular weight contaminants,such as unincorporated dye terminators, dNTPs, and residual salts from the sequencing reaction products.
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UltraClear Protocol
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Dilute Sequencing Reactions
Load Ultra Clear Plate
Centrifuge
Add 40 µl Sequencing Solution
Resuspend the Purified Sequencing Products
Transfer Sequencing Product to an Appropriate Injection Plate for each configuration.
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Features and Benefits
- Excellent quality – PHRED q>20 scores greater than 850 bases (See Data)
- Scientifically engineered to use less BigDye® version 3.1 chemistry and save money
- Optimized centrifugation procedures for maximum flexibility
- Partial 96-well plate can be used and stored at room temperature
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Order Information
| Product Code |
Product Name |
Size |
| UC9601 |
UltraClear Sequencing Reaction Clean-Up |
1 x 96 |
| UC9604 |
UltraClear Sequencing Reaction Clean-Up |
4 x 96 |
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High Quality Sequence Data with UltraClear Kit
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Excellent PHRED q>20 Scores Compared to Competition
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Data quality analysis based on PHRED q>20 scores. Samples analyzed on the ABI Prism® 3700 under standard run conditions. M13 template was prepared using 1/8 BigDye Terminator v3.1.
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Optimized Protocol Accommodates a Variety of Spin Forces and Times
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back to DNA and RNA Purification
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