Epigenetics

Sigma has a wide portfolio of products to support Epigenetics research. The Imprint® Bisulfite Modification Kit is a rapid method to determine the methylation status of CpG islands. Sigma also has products to study Histone modification, including transcription regulators such as anti-histone antibodies.

Products: Epigenetics Products ChIP Validated Antibodies  DNA Purification Imprint® Bisulfite DNA Modification Kit Imprint® Chromoimmunoprecipitation (ChIP) Kit   Imprint® Global DNA Methylation Quantification Kit  Post Reaction  Tool Box: Your Favorite Gene Search Special Offers: 25% discount for ASHG members Technology Updates/Testimonials: Update on MOD50 Update on CHP1 Update on MDQ1

Applications Center: Protocols Literature References Videos Life Science Application Center Learning Center: Contact Us Epigenetics Overview Technologies of Interest DNA Methylation and Transcription Repression       Related Topics: Functional Genomics Gene Regulation Labware   Core Bioreagents   Grant Collaborations

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Imprint® Methylated DNA Quantification technology A rapid and reliable method to measure global DNA methylation shifts in about half of a day. Fast - The total protocol can be completed in about 4 hours Flexible - 8 well assay strips may be used for high-throughput applications or manual analysis; genomic DNA from cultured cells, tissues, plasma, or other body fluid samples may be used Convenient - This simple protocol uses a familiar ELISA-like procedure:  colorimetric quantification and no radioactivity; low DNA input from 10-200 ng Complete - Includes methylated control DNA and all necessary reagents except water Sensitive - Detection limit is 5 ng of fully methylated genomic DNA (we used human in our tests).