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Sigma® combines speed and convenience with performance in the Imprint® Chromatin Immunoprecipitation Kit (CHP1). The Imprint® ChIP Kit provides a complete solution for Chromatin Immunoprecipitation including columns and reagents for DNA purification and an integrated protocol for ChIP DNA amplification with our GenomePlex® Whole Genome Amplification Kit (WGA2). The flexible format allows for immunoprecipitation and purification of DNA from mammalian cells or tissue in a convenient strip-well format.
- Fast—Total protocol time of less than 6 hours making the Imprint® kit the fastest on the market
- Sensitive—As few as 20,000 cells required for each ChIP sample
- Convenient—Fewest steps of any available ChIP protocol
- Flexible—Protocols for cells or tissue, and convenient
strip-well format for high-throughput applications
- Complete—Includes columns and reagents for DNA purification as well as an integrated protocol for amplification with the GenomePlex technology
View the Chromatin IP (ChIP) Troubleshooting Questions
View the poster Rapid and easy method for Chromatin IP (ChIP) analysis of metastatic cells
(446 KB PDF).
View the technical article ChIP Protocol To Rapidly Characterize DNA-Protein Interactions
Figure 1. Comparison of time required for protocol completion from fixation through purification using different Chromatin Immunoprecipitation kits. Each ChIP experiment was carried out using each manufacturers recommended protocol and total required times were compared.
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Figure 2: One day is sufficient to acquire ChIP enriched DNA using Sigma's Imprint® ChIP protocol. ChIP with antibodies against mouse IgG, H3K9ac, RNAP and H3K27me3 was completed on 10,000 and 100,000 SW480 cells per reaction. A fraction (1%) of the sonicated chromatin was set aside as 'input' DNA before the antibody affi nity manipulations. DNA was purified following cross-link reversal. The resulting enriched DNA was probed for GAPDH using specific primers by qPCR. Percent input was calculated by 100 x 2^(Ctadjusted Input — CtEnriched). Input DNA Ct was adjusted from 1% to 100% equivalent by subtracting 6.644 Cts or Log2 100.
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| Figures 3 and 4. A highly sensitive ChIP assay. HeLa cells were counted, fixed, and immunoprecipitated according to the Imprint® CHP1 bulletin with optional High Sensitivity method. ChIP assays were performed using anti-H3K9ac (H9286) and kit provided antibodies against: human RNA Polymerase II and non-specific mouse IgG. To evaluate ChIP enrichment, SYBR qPCR was conducted targeting the GAPDH promoter region (highly expressed house-keeping gene). Percent Input describes the amount of DNA with and without antibody selection. For these antibodies, apparent enrichment increased with fewer cells per reaction from diminished non-specific pull-down. |
Ordering Information
Products to Support the Epigenetics Workflow using this kit
DNA extraction and modification
| Prod No. |
Product Name |
| G1N10 |
GenElute™ Mammalian Genomic DNA Miniprep Kit |
| NA2000 |
GenElute™ Blood Genomic DNA Kit |
| G2N10 |
GenElute™ Plant Genomic DNA Miniprep Kit |
| MOD50 |
Imprint® DNA Modification Kit |
DNA-protein interaction & ChIP validated antibodies
| Prod No. |
Product Name |
| A5463 |
Anti-Acetylated Proteins antibody produced in rabbit |
| H0913 |
Monoclonal Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in mouse |
| H2663 |
Monoclonal Anti-Histone Deacetylase 2 (HDAC2) antibody produced in mouse |
| H7162 |
Anti-methyl-Histone H3 (Me-Lys9) antibody produced in rabbit |
| H7789 |
Anti-acetyl-Histone H1.4 (Ac-Lys26) antibody produced in rabbit |
| H9161 |
Anti-acetyl- & phospho-Histone H3 (Ac-Lys9, pSer10) antibody produced in rabbit |
| H9286 |
Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in rabbit |
| R4279 |
Anti-RbAp46, C-terminal antibody produced in rabbit |
Mission shRNA for functional genomic studies
| Prod No. |
Product Name |
| SH3011 |
MISSION® shRNA Human Gene Family Set, Bacterial Glycerol Stock Epigenetic Regulators |
| SH3021 |
MISSION® shRNA Human Gene Family Set, DNA Epigenetic Regulators |
| SH3031 |
MISSION® shRNA Human Gene Family Set, Lentiviral Particles Epigenetic Regulators |
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