Molecular Biology

Ethidium Bromide Removal

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Selected Protocol from "Molecular Cloning: A Laboratory Manual", Third Edition, Joseph Sambrook, David W. Russell.

Removal of Ethidium Bromide from DNA by Extraction with Organic Solvents

Joseph Sambrook
Peter Maccallum Cancer Institute and The University of Melbourne, Australia
David W. Russell
University of Texas Southwestern Medical Center, Dallas

ABSTRACT
Ethidium bromide is usually removed from DNA purified through a CsCl gradient by repeated extraction with organic solvents. The CsCl is subsequently removed by dialysis or by precipitation. This method is highly effective in removing ethidium bromide from DNA purified by equilibrium centrifugation in CsCl-ethidium bromide gradients.

 

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Products Available for this Protocol

Protocol Material Description Product #  Product Name Add to Cart
Buffers and Solutions      
Ethanol E7023 Ethanol
Isoamyl alcohol or n-butanol, saturated with H2O B7906 1-Butanol
Phenol P1037 Phenol
Phenol:chloroform (1:1 v/v) P1944 Phenol:Chloroform 5:1
TE (pH 8.0) T9285 Tris-EDTA Buffer 100× Concentrate
Special Equipment      
Dialysis tubing D9277 Dialysis Tubing Cellulose Membrane
Dialysis clips Z371092 Dialysis tubing closures

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