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Molecular Biology
Ethidium Bromide Removal
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Sigma® has prepared product lists for commonly used protocols from Cold Spring Harbor Protocols. |
View the protocol on BioSupplyNet.com.
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Selected Protocol from "Molecular Cloning: A Laboratory Manual", Third Edition, Joseph Sambrook, David W. Russell.
Removal of Ethidium Bromide from DNA by Extraction with Organic Solvents
Joseph Sambrook
Peter Maccallum Cancer Institute and The University of Melbourne, Australia
David W. Russell
University of Texas Southwestern Medical Center, Dallas
ABSTRACT
Ethidium bromide is usually removed from DNA purified through a CsCl gradient by repeated extraction with organic solvents. The CsCl is subsequently removed by dialysis or by precipitation. This method is highly effective in removing ethidium bromide from DNA purified by equilibrium centrifugation in CsCl-ethidium bromide gradients.
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Products Available for this Protocol
| Protocol Material Description |
Product # |
Product Name |
Add to Cart |
| Buffers and Solutions |
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| Ethanol |
E7023 |
Ethanol |
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| Isoamyl alcohol or n-butanol, saturated with H2O |
B7906 |
1-Butanol |
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| Phenol |
P1037 |
Phenol |
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| Phenol:chloroform (1:1 v/v) |
P1944 |
Phenol:Chloroform 5:1 |
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| TE (pH 8.0) |
T9285 |
Tris-EDTA Buffer 100× Concentrate |
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| Special Equipment |
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| Dialysis tubing |
D9277 |
Dialysis Tubing Cellulose Membrane |
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| Dialysis clips |
Z371092 |
Dialysis tubing closures |
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Product Association Disclaimer: The Sigma-Aldrich products listed for this specific protocol were selected either to match or to supplement the products listed within the actual protocol. The products/reagents from Sigma-Aldrich have been qualified for usage, but may not have been validated for this specific application. Please refer to the detailed product description on the usage of specific products of interest.
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