Ethidium Bromide Removal

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Selected Protocol from "Molecular Cloning: A Laboratory Manual", Third Edition, Joseph Sambrook, David W. Russell.

Removal of Ethidium Bromide from DNA by Extraction with Organic Solvents

Joseph Sambrook
Peter Maccallum Cancer Institute and The University of Melbourne, Australia
David W. Russell
University of Texas Southwestern Medical Center, Dallas

ABSTRACT
Ethidium bromide is usually removed from DNA purified through a CsCl gradient by repeated extraction with organic solvents. The CsCl is subsequently removed by dialysis or by precipitation. This method is highly effective in removing ethidium bromide from DNA purified by equilibrium centrifugation in CsCl-ethidium bromide gradients.

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Products Available for this Protocol

Protocol Material Description	Product #	Product Name	Add to Cart
Buffers and Solutions
Ethanol	E7023	Ethanol
Isoamyl alcohol or n-butanol, saturated with H₂O	B7906	1-Butanol
Phenol	P1037	Phenol
Phenol:chloroform (1:1 v/v)	P1944	Phenol:Chloroform 5:1
TE (pH 8.0)	T9285	Tris-EDTA Buffer 100× Concentrate
Special Equipment
Dialysis tubing	D9277	Dialysis Tubing Cellulose Membrane
Dialysis clips	Z371092	Dialysis tubing closures

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