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Molecular Biology

Protein Complexes

Sigma® has prepared product lists for commonly used protocols from Cold Spring Harbor Protocols.

  View the protocol on BioSupplyNet.com.

Selected Protocol from "Proteins and Proteomics: A Laboratory Manual," Richard J. Simpson.

View the protocol "Analysis of the Topology of Protein Complexes Using Cross-Linking and Mass Spectrometry" at BioSupplynet.com

Juri Rappsilber and Matthias Mann

ABSTRACT
This protocol is designed to allow identification of spatial relationships between proteins within a wide variety of multiprotein complexes. Details for optimizing cross-linking of proteins within multiprotein complexes are provided. Following
the cross-linking reaction, the proteins are separated by one-dimensional
PAGE, the cross-linked proteins are isolated from the gel, and the individual members of the cross-linked complexes are identified by mass spectrometry. The protocol can be used to analyze many protein complexes isolated by any purification technique, provided the protein complexes remain in their native configuration.

Buy this book, Z700428

Products Available for this Protocol

Protocol Material Description Product #  Product Name Add to Cart
20 mM PIPES (pH 7.0) P1851 PIPES, Biotechnology Performance Certified, cell culture tested, ≥99% (titration)
BS³ (bis[sulfosuccinimidyl]suberate) S5799 Suberic acid bis(3-sulfo-N-hydroxysuccinimide ester) sodium salt
Dithiothreitol (DTT) D9779 DL-Dithiothreitol, for molecular biology, ≥98% (TLC), ≥99% (titration)
Tris-Cl (pH 7.8) 93313 Trizma® hydrochloride buffer solution, BioChemika Ultra, for molecular biology, pH 7.4
Dimethylsulfoxide (DMSO) (HPLC grade, if possible) 34869 Dimethyl sulfoxide, for HPLC, ≥99.7%
EDC (1-ethyl-3-[3-dimethylaminopropyl]carbodiimide) 165344 1-[3-(Dimethylamino)propyl]-3-ethylcarbodiimide methiodide
SMCC (succinimidyl-4-[N-maleimidomethyl]cyclohexane-1-carboxylate) M6035 4-(N-Maleimidomethyl)cyclohexane-1-carboxylic acid 3-sulfo-N-hydroxysuccinimide ester sodium salt
SDS L4390 Sodium dodecyl sulfate, for molecular biology, ≥98.5% (GC)
Bromophenol blue B5525 Bromophenol Blue sodium salt for electrophoresis
Glycerol G5516 Glycerol, for molecular biology, ≥99%
Acrylamide solution A4058 Acrylamide solution, 40%, for electrophoresis, sterile-filtered
Protein molecular weight marker, prestained View the entire line of protein molecular weight markers
Reagents for protein staining View the entire line staining reagents for proteins

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View all available product lists for Cold Spring Harbor Laboratory Protocols

Product Association Disclaimer: The Sigma-Aldrich products listed for this specific protocol were selected either to match or to supplement the products listed within the actual protocol. The products/reagents from Sigma-Aldrich have been qualified for usage, but may not have been validated for this specific application. Please refer to the detailed product description on the usage of specific products of interest.