Purification of PCR Products

Selected Protocol from "Molecular Cloning: A Laboratory Manual", Third Edition, Joseph Sambrook, David W. Russell. Purification of PCR Products in Preparation for Cloning Joseph Sambrook Peter Maccallum Cancer Institute and The University of Melbourne, Australia David W. Russell University of Texas Southwestern Medical Center, Dallas ABSTRACT The residual enzymatic activity of thermostable DNA polymerases that survive the rigors of PCR can compromise subsequent enzymatic reactions. This protocol describes how to use proteinase K to destroy thermostable enzymes and to purify amplified DNA in preparation for cloning.

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Products Available for this Protocol

Note 1: For Step 6 of the protocol, you may use the GenElute™ PCR Clean-Up Kit, NA1020.

Note 2: As an alternative, the entire protocol could be accomplished with procuct NA1020, GenElute™ PCR Clean-Up Kit.

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